Catalase immobilization—A review

Grigoras, Anca Giorgiana

doi:10.1016/j.bej.2016.10.021

Cited by 132 publications

(68 citation statements)

References 120 publications

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“…Further work remains however to demonstrate that biocatalysis is economically competitive in other industries, such as natural gas conversion and biofuel production [13][14][15][16][17][18][19]. Moreover, across the various industries where biocatalysis can be used, a recurring barrier persists, i.e., the application of enzyme catalysis in chemical processes is limited by the lack of enzyme stability at high temperatures or in turbulent flow regimes, as well as in potentially toxic solvents [20][21][22][23][24][25][26][27]. Thus, concentrated approaches spanning over various disciplines are focusing on the identification and production of robust, stable biocatalysts suitable for application in a broader range of industrial settings [1,3,[20][21][22][23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks

2018

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Enzymes as industrial biocatalysts offer numerous advantages over traditional chemical processes with respect to sustainability and process efficiency. Enzyme catalysis has been scaled up for commercial processes in the pharmaceutical, food and beverage industries, although further enhancements in stability and biocatalyst functionality are required for optimal biocatalytic processes in the energy sector for biofuel production and in natural gas conversion. The technical barriers associated with the implementation of immobilized enzymes suggest that a multidisciplinary approach is necessary for the development of immobilized biocatalysts applicable in such industrial-scale processes. Specifically, the overlap of technical expertise in enzyme immobilization, protein and process engineering will define the next generation of immobilized biocatalysts and the successful scale-up of their induced processes. This review discusses how biocatalysis has been successfully deployed, how enzyme immobilization can improve industrial processes, as well as focuses on the analysis tools critical for the multi-scale implementation of enzyme immobilization for increased product yield at maximum market profitability and minimum logistical burden on the environment and user.

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Section: Introductionmentioning

confidence: 99%

Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks

2018

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“…There are three main methods for enzyme immobilization, including cross-linking, adsorption, and embedding, but none is generally valid for a specic enzyme. 9 Different immobilization methods indicate the different interaction between enzyme and the carrier material. 10 The activity of immobilized catalase is affected by the immobilization method.…”

Section: Introductionmentioning

confidence: 99%

Characterization of biocompatible pig skin collagen and application of collagen-based films for enzyme immobilization

Lan

Zhao

et al. 2020

RSC Adv.

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Based on the excellent biocompatibility of collagen, collagen was extracted from pig skin by acid-enzymatic method. The films were prepared by the self-aggregation behavior of collagen, and the catalase was immobilized by adsorption, cross-linking and embedding. The experiment investigated the effects of glutaraldehyde on the mechanical properties, external sensory properties, and denaturation temperature of the films. The results showed that self-aggregating material could maintain the triple helix structure of pig skin collagen. The self-aggregation treatment and cross-linking treatment can improve the mechanical properties to 53 MPa, while the glutaraldehyde cross-linking agent can increase the denaturation temperature of the pig skin collagen self-aggregating membrane by 20.35% to 84.48 C.This means that its application to immobilized catalase has better stability. The comparison shows that the catalase immobilized by the adsorption method has strong activity and high operational stability, and the cross-linking agent glutaraldehyde and the initial enzyme concentration have a significant effect on the immobilization, and the activity can reach 175 U g À1 . After 16 uses of the film, the catalase was completely inactivated. This study provides a reference for the preparation of a catalase sensor that can be used to detect hydrogen peroxide in food by a catalase sensor.

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“…Several immobilization methods have been used, to date, including adsorption, covalent linkage, affinity immobilization, entrapment, encapsulation, and enzyme crosslinking . Among them, simple entrapment offers easy fabrication and handling, and endows high stability to the immobilized enzymes . Regarding the support, the use of natural materials as scaffolds provides advantages of biocompatibility, chemical versatility, and biodegradability that organic or inorganic support materials do not offer.…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic Protein‐Based Materials for Integration into Energy Devices

et al. 2019

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There is a current need to fabricate new biobased functional materials. Bottom‐up approaches to assemble simple molecular units have shown promise for biomaterial fabrication due to their tunability and versatility for the incorporation of functionalities. Herein, the fabrication of catalytic protein thin films by the entrapment of catalase into protein films composed of a scaffolding protein is demonstrated. Extensive structural and functional characterization of the films provide evidence of the structural integrity, order, stability, catalytic activity, and reusability of the biocatalytic materials. Finally, these functional biomaterials are coupled with piezoelectric disks to fabricate a second generation of bio‐inorganic generators. These devices are capable of producing electricity from renewable fuels through catalase‐driven gas production that mechanically stimulates the piezoelectric material.

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Catalase immobilization—A review

Cited by 132 publications

References 120 publications

Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks

Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks

Characterization of biocompatible pig skin collagen and application of collagen-based films for enzyme immobilization

Biocatalytic Protein‐Based Materials for Integration into Energy Devices

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