Catalase/Fe3O4@Cu2+ hybrid biocatalytic nanoflowers fabrication and efficiency in the reduction of organic pollutants

Alhayali, Noor Ibrahim; Özpozan, Nilgün; Dayan, Serkan; Özdemir, Nalan; Yılmaz, Burcu Somtürk

doi:10.1016/j.poly.2020.114888

Cited by 26 publications

(9 citation statements)

References 42 publications

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“…In the region of 1200-950 (cm −1 ), there is a robust characteristic peak that can be attributed to vibrations and elongations of P-O, found in the presence of phosphate groups [12,21,38]. The relatively small band at 530-670 (cm −1 ) was likely the phosphorus bridge bending vibrations, such as O=P-O, contributing to the phosphate groups [25,39]. Characteristic peaks are found due to the PO 4 −3 vibration, evidencing the existence of PBS-Cu 2+ in the analyzed nanoparticle (Figure 6).…”

Section: Ftir Of Hnf-lipasementioning

confidence: 99%

Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization

Souza,

Santos,

Freitas

et al. 2024

Molecules

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This work presents a framework for evaluating hybrid nanoflowers using Burkholderia cepacia lipase. It was expanded on previous findings by testing lipase hybrid nanoflowers (hNF-lipase) formation over a wide range of pH values (5–9) and buffer concentrations (10–100 mM). The free enzyme activity was compared with that of hNF-lipase. The analysis, performed by molecular docking, described the effect of lipase interaction with copper ions. The morphological characterization of hNF-lipase was performed using scanning electron microscopy. Fourier Transform Infrared Spectroscopy performed the physical–chemical characterization. The results show that all hNF-lipase activity presented values higher than that of the free enzyme. Activity is higher at pH 7.4 and has the highest buffer concentration of 100 mM. Molecular docking analysis has been used to understand the effect of enzyme protonation on hNF-lipase formation and identify the main the main binding sites of the enzyme with copper ions. The hNF-lipase nanostructures show the shape of flowers in their micrographs from pH 6 to 8. The spectra of the nanoflowers present peaks typical of the amide regions I and II, current in lipase, and areas with P–O vibrations, confirming the presence of the phosphate group. Therefore, hNF-lipase is an efficient biocatalyst with increased catalytic activity, good nanostructure formation, and improved stability.

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Section: Ftir Of Hnf-lipasementioning

confidence: 99%

Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization

Souza,

Santos,

Freitas

et al. 2024

Molecules

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“…flower-like [ 1 , 2 ], hollow-yolk shell [ 3 ], core-shell [ 4 ] and nanorods [ 5 ] are reported. These nanostructures have some unique features such as fast charge transfer, the reduced recombination rate of charge carriers and high surface area which allows their utilization in adsorption and photocatalysis [ 6 ]. For enhancing the activity of these nanocomposites, the right choice of the components is an important aspect.…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Nanoflowers of MgFe2O4, Bentonite and B-,P- Co-Doped Graphene Oxide as Adsorbent and Photocatalyst: Optimization of Parameters by Box–Behnken Methodology

Ubhi

Kaur

Singh

et al. 2022

IJMS

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In the present study, nanocomposites having hierarchical nanoflowers (HNFs) -like morphology were synthesized by ultra-sonication approach. HNFs were ternary composite of MgFe2O4 and bentonite with boron-, phosphorous- co-doped graphene oxide (BPGO). The HNFs were fully characterized using different analytical tools viz. X-ray photoelectron spectroscopy, scanning electron microscopy, energy dispersion spectroscopy, transmission electron microscopy, X-ray diffraction, vibrating sample magnetometry and Mössbauer analysis. Transmission electron micrographs showed that chiffon-like BPGO nanosheets were wrapped on the MgFe2O4-bentonite surface, resulting in a porous flower-like morphology. The red-shift in XPS binding energies of HNFs as compared to MgFe2O4-bentoniteand BPGO revealed the presence of strong interactions between the two materials. Box–Behnken statistical methodology was employed to optimize adsorptive and photocatalytic parameters using Pb(II) and malathion as model pollutants, respectively. HNFs exhibited excellent adsorption ability for Pb(II) ions, with the Langmuir adsorption capacity of 654 mg g−1 at optimized pH 6.0 and 96% photocatalytic degradation of malathion at pH 9.0 as compared to MgFe2O4-bentonite and BPGO. Results obtained in this study clearly indicate that HNFs are promising nanocomposite for the removal of inorganic and organic contaminants from the aqueous solutions.

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“…The catalase/Fe 3 O 4 @Cu 2+ prepared by Alhayali et al . has a good reducing ability for nitrobenzenes and organic compounds 35 . Some studies have indicated that catalase as a feed additive could prevent and control mycotoxin contamination, clean mycotoxins and improve animal resistance 36,37 .…”

Section: Introductionmentioning

confidence: 99%

“…34 The catalase/Fe 3 O 4 @Cu 2+ prepared by Alhayali et al has a good reducing ability for nitrobenzenes and organic compounds. 35 Some studies have indicated that catalase as a feed additive could prevent and control mycotoxin contamination, clean mycotoxins and improve animal resistance. 36,37 In this study, the catalase gene (KatA) from Pseudomonas aeruginosa was cloned, and the recombinant catalase that could degrade AFB 1 was obtained by expressing it in E. coli.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB₁ by recombinant catalase

Lou

Zhao

2022

J Sci Food Agric

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BACKGROUND Aflatoxin B1 (AFB1) poses a severe threat to human and animal health. Countries worldwide have invested considerable manpower and material resources in degrading aflatoxins. Enzyme degradation is the most efficient and environmentally friendly approach for modifying aflatoxin into less toxic molecules. Catalase is commonly used as a detoxification agent to decrease the contamination levels of aflatoxins in animal feeds. This study aimed to obtain recombinant catalase via gene engineering and determined whether a recombinant catalase could degrade AFB1. RESULTS The catalase gene (KatA) from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli, and the expression conditions of this recombinant catalase were optimized. The recombinant catalase was isolated and purified using Ni‐chelating affinity chromatography, and its ability to degrade AFB1 was evaluated. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis showed that the expressed of catalase was approximately 55.6 kDa, which was subsequently purified using Ni‐chelating affinity chromatography. The degradation rate of AFB1 by recombinant catalase in the presence of syringaldehyde was 38.79%. CONCLUSION The degradation of AFB1 by a recombinant catalase has been reported for the first time. This study provides a new paradigm for the use of recombinant catalases in degrading AFB1 in food and feed. © 2022 Society of Chemical Industry.

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Catalase/Fe3O4@Cu2+ hybrid biocatalytic nanoflowers fabrication and efficiency in the reduction of organic pollutants

Cited by 26 publications

References 42 publications

Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization

Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization

Hierarchical Nanoflowers of MgFe2O4, Bentonite and B-,P- Co-Doped Graphene Oxide as Adsorbent and Photocatalyst: Optimization of Parameters by Box–Behnken Methodology

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB₁ by recombinant catalase

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Catalase/Fe3O4@Cu2+ hybrid biocatalytic nanoflowers fabrication and efficiency in the reduction of organic pollutants

Cited by 26 publications

References 42 publications

Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization

Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization

Hierarchical Nanoflowers of MgFe2O4, Bentonite and B-,P- Co-Doped Graphene Oxide as Adsorbent and Photocatalyst: Optimization of Parameters by Box–Behnken Methodology

Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB1 by recombinant catalase

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Cloning and expression of the catalase gene (KatA) from Pseudomonas aeruginosa and the degradation of AFB₁ by recombinant catalase