Catalase Activity in Red Cell and Liver of Mice Infected with Plasmodium Berghei

Areekul, S; Boonme, Yupin

doi:10.1007/978-3-0348-7432-8_34

Cited by 9 publications

(15 citation statements)

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“…This claim was further supported by decrease in red cell and hepatic activities of SOD and CAT, and hepatic GST of infected mice, which indicate that excess ROS probably inactivate these antioxidant enzymes. This observation is in line with the findings of Areekul and Boonme (1986) that linked the reduced activity of catalase in P. berghei infection to excessive ROS generation. We assessed the effect of MENS on lipid peroxidation and antioxidant enzymes of the infected mice.…”

Section: Discussionsupporting

confidence: 92%

Antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (black cumin) in mice infected with Plasmodium yoelli nigeriensis

et al. 2010

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The antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (MENS) were investigated against established malaria infection in vivo using Swiss albino mice. The antimalarial activity of the extract against Plasmodium yoelli nigeriensis (P. yoelli) was assessed using the Rane test procedure. Chloroquine (CQ)-treated group served as positive control. The extract, at a dose of 1.25 g/kg body weight significantly (p<0.05) suppressed P. yoelli infection in the mice by 94%, while CQ, the reference drug, produced 86% suppression when compared to the untreated group after the fifth day of treatment. P. yoelli infection caused a significant (p<0.05) increase in the levels of red cell and hepatic malondialdehyde (MDA), an index of lipid peroxidation (LPO) in the mice. Serum and hepatic LPO levels were increased by 71% and 113%, respectively, in the untreated infected mice. Furthermore, P. yoelli infection caused a significant (p<0.05) decrease in the activities of superoxide dismutase, catalase, glutathione-S-transferase and the level of reduced glutathione in tissues of the mice. Treatment with MENS significantly (p<0.05) attenuated the serum and hepatic MDA levels in P. yoelli-infected mice. In addition, MENS restored the activities of red cell antioxidant enzymes in the infected mice to near normal. Moreover, MENS was found to be more effective than CQ in parasite clearance and, in the restoration of altered biochemical indices by P. yoelli infection. These results suggest that N. sativa seeds have strong antioxidant property and, may be a good phytotherapeutic agent against Plasmodium infection in malaria.

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Section: Discussionsupporting

confidence: 92%

Antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (black cumin) in mice infected with Plasmodium yoelli nigeriensis

et al. 2010

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“…The activity of catalase is decreased in P. berghei infection (Areekul and Boonme 1986). We confirmed this evidence, and this could promote an increase in the levels of free radicals.…”

Section: Discussionsupporting

confidence: 76%

Effect of dequalinium on the oxidative stress in Plasmodium berghei-infected erythrocytes

Rodrígues

Gamboa

2009

Parasitol Res

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The bisquinoline drug dequalinium (DQ) has demonstrated remarkable activity against some infection diseases, including malaria. Oxidative stress represents a biochemical target for potential antimalarials. In this work, we have tested the ability of this compound to modify the oxidative status in Plasmodium berghei-infected erythrocytes. After hemolysis, activities of superoxide dismutase (SOD), catalase (CAT), glutathione cycle, and dehydrogenase enzymes were investigated. The activity of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGLD) in infected cells were diminished by this drug compared to controls (300% and 80% approximately, respectively), while glutathione peroxidase (GPx), glutathione transferase (GST), and glutathione levels were also lowered. As a compensatory response, we could appreciate an increase of SOD activity (20% approximately) in infected cells treated with DQ; however, catalase was not affected by the compound. Lipid peroxidation was also decreased by this drug, protecting the cells from the hemolysis caused by the infection. In conclusion, oxidative stress represents a biochemical event which is modulated by DQ, interfering with the antioxidant regular activities in P. berghei infection.

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“…However, this "adopted" SOD may be superfluous since it resides in the acidic food vacuole of the parasite where spontaneous dismutation (accelerated by H + ) may be sufficient to transform 0 2 to H2O2. In comparison, SOD levels in P. berghei IRBC were found to be increased (Seth et al, 1985) and it has been recently demonstrated that murine parasites contain a Fe-SOD, in contradistinc tion with that of the host cell which is a Cu, Zn-SOD (Becuwe et al, 1993)· The levels of catalase increase in P. falciparum (Fairfield et al, 1988) but decrease in P. berghei (Areekul & Boonme, 1992).…”

Section: Antioxidant Enzymesmentioning

confidence: 97%

The redox status of malaria-infected erythrocytes: an overview with an emphasis on unresolved problems

Ginsburg

Atamna

1994

Parasite

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Summary :The various mechanisms involved in the redox defence of norma erythrocytes are adequately known. They are herein briefly reviewed, outlining the principal enzymes and metabolic pathways, such as superoxide dismutase, catalase, glutathione peroxidase and reductase, the hexose monophosphate shunt (HMS) and glutathione synthesis and turnover. The intraerythrocytic malaria parasite is imposing an oxidative stress on its host cell. Malaria-infected cells produce O 2 -, H 2 O 2 , enhance lipide peroxidation and activate host cell HMS. This stress is produced during the digestion of host cell hemoglobin by the parasite. Hence, both parasite and host cell must be able to confront this stress. The antioxidant defence systems of the parasite and the response of those systems in the infected host cell are reviewed, underscoring unresolved problems. Nothing is virtually known on the parasite's glutathione metabolism, and on possible interactions between host cell and parasite antioxidant defence systems. The postulate that 1. host cell activated HMS in conjunction with purine salvage can provide purine nucleotides to the parasite, and 2. that glutathione transferase can participate in parasite resistance to antimalarial drugs, are also discussed.T he redox status of malaria infected erythrocytes has been the subject of intense investigations and reviews in past years (Vennerstrom & Eaton, 1988 ; Golenser & Chevion, 1989 ; Hunt & Stocker, 1990). It is generally accepted that the intraerythrocytic malaria parasite exerts an oxidative stress on its host erythrocyte ; that externally applied oxidative stress is antagonistic to survival and propagation of the malaria parasite, and that pro-oxidant agents are effective inhibitors of parasite growth in vitro and in vivo. The host's response to malaria involves, among others, oxidative attack on intraerythrocytic parasites by activated macrophages. This perception is based mostly on phenomenological observations, but relatively little is known on the redox metabolism of the parasite itself, or how the latter may affect that of the host cell. In this essay we shall briefly review our present knowledge and underscore those problems Rice-Evans, 1991), and therefore RBC are exquisitely well equipped to counteract the damage that can be produced by these radicals (Stern, 1985).Superoxide anions (O2 -) produced during the spontaneous oxidation of hemoglobin to methemoglobin are dismutated to H 2 C>2 by superoxide dismutase (SOD).

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Catalase Activity in Red Cell and Liver of Mice Infected with Plasmodium Berghei

Cited by 9 publications

References 9 publications

Antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (black cumin) in mice infected with Plasmodium yoelli nigeriensis

Antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (black cumin) in mice infected with Plasmodium yoelli nigeriensis

Effect of dequalinium on the oxidative stress in Plasmodium berghei-infected erythrocytes

The redox status of malaria-infected erythrocytes: an overview with an emphasis on unresolved problems

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