Catalase Activity in Brown Mussels (Perna perna) under Acute Cadmium, Lead, and Copper Exposure and Depuration Tests

Abstract

Brown mussels (Perna perna) were exposed to cadmium (Cd), lead (Pb), and copper (Cu) concentrations under acute exposure and exposure-depuration tests for the estimation of biochemical biomarker catalase (CAT). The acute tests showed accumulated Cd, Pb, and Cu inPerna pernacorrelated linearly with the exposure concentrations (R2=0.794,R2=0.891, andR2=0.985for Cd, Pb, and Cu, resp.). The results of CAT increased significantly in tissues of treatment mussels after 72 h exposure when compared to control. The values of total protein were disturbed in exposed groups when compared with control. These results suggest that metabolites and catalase activity were affected by heavy metal exposures. Analysis using the Spearman rank correlation coefficient showed that the CAT activity appeared to have a significant positive correlativity (Rs=0.921,Rs=0.949, andRs=0.949for Cd, Pb, and Cu, resp.) with the accumulated Cd, Pb, and Cu concentrations, respectively. The result of exposure-depuration tests showed that there is a general tendency for CAT to decrease in depuration phase, suggesting that the induction of catalase is metal and/or mixture of metals dependent.

Effect of Heavy Metals on the Activity of Catalase and Glutathione-S-Transferase in Nile Tilapia Fish (Oreochromis niloticus)

“…Our findings contradicted with previous publication, which showed that Cu accumulation has no correlation with GST activity in different tissues of this species collected from the Nhue-Day River [8]. However, our results were in line with Cu and Pb but not with Cd treatment for Brown Mussels (Perna perna), which showed a high correlation (R>0.92, p<0.01) of the CAT activity in tissues with the metal accumulation [15]. Together with these findings, our data might suggests enzyme activity was changed not only by the accumulation of metal but also by other factors such as the form of metal chemicals, characteristics of organisms, and the living environment as reported in previous study [4,12,13].…”

“…Our data displayed not only the activation but also the repression of enzyme activity by HMs exposure, suggesting that the activity changes might be related to the accumulation of HMs in the organism. Many scientists have been concerned about the relationship between HMs accumulation and enzyme activity in various species [8,14,15]. To examine whether the accumulation of Cu, Pb, and Cd affects alteration in the activity of CAT and GST, the Pearson's correlation test for the relationship between enzyme activity and HMs accumulation in muscle was performed (data not shown).…”

Toxic Effects and Depuration on the Antioxidant and Neurotransmitter Responses after Dietary Lead Exposure in Starry Flounder

“…Boudjema et al. () have also reported that CAT activity was restored after the depuration period in brown mussels Perna perna after acute exposure to Cd, Pb, and Cu. Therefore, the fish tissues analyzed in the present study were able to adapt to Pb concentrations of 120 mg/kg efficiently, and this concentration might indicate the peak capacity of Starry Flounder against the dietary Pb exposure to resist oxidative stress.…”

Antioxidant defenses of flame scallop Ctenoides scaber (Born, 1778) exposed to the water-soluble fraction of used vehicle crankcase oils

“…The overproduction of H 2 O 2 is countered by an increase of CAT activity, which it is part of biochemical adjustments against the ROS generation in the digestive gland. Similarly, other authors had demonstrated that SOD and CAT activities increase in diverse invertebrate’s species such as gastropod [ 48 ], mussels [ 49 ], clam [ 50 ] and scallops [ 51 ] exposed to heavy metals and mixtures of polyaromatic hydrocarbons under acute exposure.…”