Catabolism of protocatechuate by Bacillus macerans

Crawford, R.L.; Bromley, J W; Perkins-Olson, P E

doi:10.1128/aem.37.3.614-618.1979

Cited by 35 publications

(12 citation statements)

References 14 publications

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“…These observations were supported by correlating their abundance distribution. The abundances of the film-associated taxa (bins 3, 5, 16,28,32) are significantly positively-correlated to each other. Similarly, the abundances of free-living taxa (1,2,9,10,12,13,14,15,17,21,23,26,30,33) are positively correlated with each other.…”

Section: Resultsmentioning

confidence: 92%

Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium

et al. 2020

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The degradation of synthetic polymers by marine microorganisms is not as well understood as the degradation of plastics in soil and compost. Here, we use metagenomics, metatranscriptomics and metaproteomics to study the biodegradation of an aromatic-aliphatic copolyester blend by a marine microbial enrichment culture. The culture can use the plastic film as the sole carbon source, reaching maximum conversion to CO2 and biomass in around 15 days. The consortium degrades the polymer synergistically, with different degradation steps being performed by different community members. We identify six putative PETase-like enzymes and four putative MHETase-like enzymes, with the potential to degrade aliphatic-aromatic polymers and their degradation products, respectively. Our results show that, although there are multiple genes and organisms with the potential to perform each degradation step, only a few are active during biodegradation.

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Section: Resultsmentioning

confidence: 92%

Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium

et al. 2020

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“…Cell extracts were examined for the presence of the following enzymes: 4,5-dihydroxyphthalate decarboxylase, 3,4-dihydroxyphthalate decarboxylase, protocatechuate 4,5-dioxygenase, protocatechuate 3,4-dioxygenase, and protocatechuate 2,3-dioxygenase. Assays were performed by monitoring disappearance of substrates and appearance of products by UVvisible spectrometry, on the basis of spectral properties reported by others for these enzyme systems (8,11,29,31). Spectral changes at 30°C were recorded with a Perkin-Elmer Lambda 6 spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Evidence for a novel pathway in the degradation of fluorene by Pseudomonas sp. strain F274

Grifoll

Selifonov

Chapman

1994

Appl Environ Microbiol

106

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A fluorene-utilizing microorganism, identified as a species of Pseudomonas, was isolated from soil severely contaminated from creosote use and was shown to accumulate six major metabolites from fluorene in washed-cell incubations. Five of these products were identified as 9-fluorenol, 9-fluorenone, (+)-1,ladihydroxy-1-hydro-9-fluorenone, 8-hydroxy-3,4-benzocoumarin, and phthalic acid. This last compound was also identified in growing cultures supported by fluorene. Fluorene assimilation into cell biomass was estimated to be approximately 50%. The structures of accumulated products indicate that a previously undescribed pathway of fluorene catabolism is employed by Pseudomonas sp. strain F274. This pathway involves oxygenation of fluorene at C-9 to give 9-fluorenol, which is then dehydrogenated to the corresponding ketone, 9-fluorenone. Dioxygenase attack on 9-fluorenone adjacent to the carbonyl group gives an angular diol, l,la-dihydroxy-1-hydro-9-fluorenone. Identification of 8-hydroxy-3,4-benzocoumarin and phthalic acid suggests that the five-membered ring of the angular diol is opened first and that the resulting 2'-carboxy derivative of 2,3-dihydroxy-biphenyl is catabolized by reactions analogous to those of biphenyl degradation, leading to the formation of phthalic acid. Cell extracts of fluorene-grown cells possessed high levels of an enzyme characteristic of phthalate catabolism, 4,5-dihydroxyphthalate decarboxylase, together with protocatechuate 4,5-dioxygenase. On the basis of these findings, a pathway of fluorene degradation is proposed to account for its conversion to intermediary metabolites. A range of compounds with structures similar to that of fluorene was acted on by fluorene-grown cells to give products consistent with the initial reactions proposed.

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“…B . macerans JJ-lb degraded protocatechuate via ring cleavage and subsequent enzymatic decarboxylation of the ring fission product (Crawford et al, 1979 ). Initially, protocatechuate-2,3-dioxygenase catalyzes the ring cleavage of protocatechuate to 5-carboxy-2-hydroxymuconic semialdehyde that is further decarboxylated to 2-hydroxymuconic semialdehyde.…”

Section: Role Of Bacillus Species In Biodegradatiomentioning

confidence: 99%

Bacilli-Mediated Degradation of Xenobiotic Compounds and Heavy Metals

Arora

2020

Front. Bioeng. Biotechnol.

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Xenobiotic compounds are man-made compounds and widely used in dyes, drugs, pesticides, herbicides, insecticides, explosives, and other industrial chemicals. These compounds have been released into our soil and water due to anthropogenic activities and improper waste disposal practices and cause serious damage to aquatic and terrestrial ecosystems due to their toxic nature. The United States Environmental Protection Agency (USEPA) has listed several toxic substances as priority pollutants. Bacterial remediation is identified as an emerging technique to remove these substances from the environment. Many bacterial genera are actively involved in the degradation of toxic substances. Among the bacterial genera, the members of the genus Bacillus have a great potential to degrade or transform various toxic substances. Many Bacilli have been isolated and characterized by their ability to degrade or transform a wide range of compounds including both naturally occurring substances and xenobiotic compounds. This review describes the biodegradation potentials of Bacilli toward various toxic substances, including 4-chloro-2-nitrophenol, insecticides, pesticides, herbicides, explosives, drugs, polycyclic aromatic compounds, heavy metals, azo dyes, and aromatic acids. Besides, the advanced technologies used for bioremediation of environmental pollutants using Bacilli are also briefly described. This review will increase our understanding of Bacilli -mediated degradation of xenobiotic compounds and heavy metals.

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Catabolism of protocatechuate by Bacillus macerans

Cited by 35 publications

References 14 publications

Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium

Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium

Evidence for a novel pathway in the degradation of fluorene by Pseudomonas sp. strain F274

Bacilli-Mediated Degradation of Xenobiotic Compounds and Heavy Metals

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