Catabolism of Nitriles in Rhodococcus

Martı́nková, Ludmila; Pátek, Miroslav; Veselá, A; Kaplan, Ondřej; Uhnáková, Bronislava; Nešvera, Jan

doi:10.1007/978-3-642-12937-7_7

Cited by 8 publications

(9 citation statements)

References 145 publications

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“…Despite the genetic similarity, wide phenotypic variation is observed in specificity to aromatic, aliphatic, alicyclic and branched nitriles . Functionally associated genes in nitrile biosynthesis and degradation are clustered in all strains cloned and characterised, including Rhodococcus erythropolis PR44 (Fig.…”

Section: Nitrilesmentioning

confidence: 99%

“…Functionally associated genes in nitrile biosynthesis and degradation are clustered in all strains cloned and characterised, including Rhodococcus erythropolis PR44 (Fig. ), Rhodococcus globerulus A4, Rhodococcus sp N771 and Rhodococcus RHA1 . The action of aldoxime dehydratase (oxd), a lyase that cleaves the hydroxyl function (Fig.…”

Section: Nitrilesmentioning

confidence: 99%

“…Substrate specificity for short‐chain aliphatic amides has been demonstrated for Rhodococcus sp. R312 with an appreciably greater activity in the conversion of propionamide relative to butyramide …”

Section: Nitrilesmentioning

confidence: 99%

“…Moreover, laboratory scale testing has identified the commercial potential of nitrile hydrolysis in the production of antibiotics and anti‐inflammatories . Mayaux and co‐workers were the first to demonstrate enantioselective oxidation of α‐methylphenylacetamides and α‐methoxyphenylacetamides using enzymes from two Rhodococcus strains, while studies have now established NHase and amidase activity in the production of α ‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (a receptor antagonist) and α ‐arylpropionic acids (non‐steroid anti‐inflammatory agents) . With respect to bioremediation, benzonitrile derivatives (chloroxynil, bromoxynil, ioxynil) used as herbicides are of increasing concern due to the accumulation of persistent metabolites.…”

Section: Nitrilesmentioning

confidence: 99%

“…Nitrilase enzymes (from Rhodococcus rhodochrous PA‐34 and Rhodococcus sp. NDB 1165) can be applied to convert these herbicides into the corresponding substituted benzoic acids, which are readily mineralised . NHase‐amidase coupled reactions may not be applicable as the amide intermediate, as in the case of the herbicide dichlobenil (2,6‐dichlorobenzonitrile), can represent an even more serious contaminant .…”

Section: Nitrilesmentioning

confidence: 99%

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The repertoire of nitrogen assimilation in Rhodococcus: catalysis, pathways and relevance in biotechnology and bioremediation

Foster

Barnes

Speight

et al. 2014

J of Chemical Tech & Biotech

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The Rhodococcus genus exhibits diverse enzymatic activity that can be exploited in the conversion of natural and anthropogenic nitrogenous compounds. This catalytic response provides a selective advantage in terms of available nutrients while also serving to remove otherwise harmful xenobiotics. This review provides a critical assessment of the literature on bioconversion of organonitrogen compounds with a consideration of applications in bioremediation and commercial biotechnology. By examining the major nitro-organic compounds (amino acids, amines, nitriles, amides and nitroaromatics) in turn, the considerable repertoire of Rhodococcus spp. is established. The available published enzyme reaction data is coupled with genomic characterisation to provide a molecular basis for Rhodococcus enzyme activity with an assessment of the cellular properties that aid substrate accessibility and ensure stability. The metabolic gene clusters associated with the observed reaction pathways are identified and future directions in enzyme optimisation and metabolic engineering are assessed.Alanine dehydrogenase (RHA1_ro01495, ald1, ald2) and aspartate ammonia lyase (aspA1, aspA2, aspA3), with corresponding transaminases, provide alternative ping-pong (bi-bi) systems. Specificity and cellular catalytic efficiency is extended by a number of genes encoding asparagine dehydrogenase, arginine, cysteine, histidine and serine ammonia lyase ( Table 2). The production of

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Section: Nitrilesmentioning

confidence: 99%

Section: Nitrilesmentioning

confidence: 99%

Section: Nitrilesmentioning

confidence: 99%