Catabolic Potential of Pseudomonas cepacia

Lessie, T G; Gaffney, Thomas

doi:10.1016/b978-0-12-307210-8.50018-x

Cited by 43 publications

(61 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also probed restriction enzyme digests of the three plasmids with variants of the broad-host-range drug resistance plasmid pRP1 carrying IS401, IS402, and IS408, three insertion sequences present in the P. cepacia genome (2,21,34). The results, which are described below, are consistent with the interpretation that pTGL5 evolved from the IS408-containing plasmid pTGL1 by insertion of IS401 and IS402 at multiple sites on pTGL1 and that pTGL6 was formed from pTGL5 as a consequence of a single deletion event involving recombination between two IS401 elements.…”

Section: Resultsmentioning

confidence: 99%

“…Derivatives of the broad-host-range plasmid pRPl carrying the above insertion sequences and recombinant plasmids carrying fragments of plasmids pTGL6 and pTGL5 were used as probes to ascertain the extent of reiteration of the various elements in the P. cepacia genome. The data indicate a high frequency of genomic rearrangements which presumably contributes to the extraordinary adaptability of this bacterium.Pseudomonas cepacia is notable for the extraordinary number of compounds it can utilize as sole carbon and energy sources (1,4,17,21,26,27,36). It is both an opportunistic pathogen for humans and a phytopathogen (1,14,26 (18) was used for the isolation of recombinant derivatives of plasmids pACYC184 (8) and pBR325 (7) carrying fragments of pTGL5 and pTGL6.…”

mentioning

confidence: 99%

“…Pseudomonas cepacia is notable for the extraordinary number of compounds it can utilize as sole carbon and energy sources (1,4,17,21,26,27,36). It is both an opportunistic pathogen for humans and a phytopathogen (1,14,26 (18) was used for the isolation of recombinant derivatives of plasmids pACYC184 (8) and pBR325 (7) carrying fragments of pTGL5 and pTGL6.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Insertion-sequence-dependent rearrangements of Pseudomonas cepacia plasmid pTGL1

Gaffney¹,

Lessie²

1987

J Bacteriol

View full text Add to dashboard Cite

Pseudomonas cepacia 249 (ATCC 17616) harbors a 170-kilobase (kb) plasmid designated pTGL1. We identified three insertion sequences, IS405, IS408, and IS411, on this plasmid. Various prototrophic and auxotrophic derivatives in our collection contained variants of pTGL1 formed by accretion and deletion of other elements. Plasmid pTGL6, the variant in one prototroph, evolved from pTGL1 by the addition of three copies of IS401 (1.3 kb) and one of IS402 (1 kb), to generate pTGL5, and recombination between two of the copies of IS401 on pTGL5 to form pTGL6. The latter event entailed loss of one copy of IS401 and an additional 5.4 kb of plasmid DNA. Derivatives of the broad-host-range plasmid pRPl carrying the above insertion sequences and recombinant plasmids carrying fragments of plasmids pTGL6 and pTGL5 were used as probes to ascertain the extent of reiteration of the various elements in the P. cepacia genome. The data indicate a high frequency of genomic rearrangements which presumably contributes to the extraordinary adaptability of this bacterium.Pseudomonas cepacia is notable for the extraordinary number of compounds it can utilize as sole carbon and energy sources (1,4,17,21,26,27,36). It is both an opportunistic pathogen for humans and a phytopathogen (1,14,26 (18) was used for the isolation of recombinant derivatives of plasmids pACYC184 (8) and pBR325 (7) carrying fragments of pTGL5 and pTGL6. These and other plasmids described in this paper are listed in Tables 1 and 2. Bacteria were grown at 37°C with shaking in flasks filled to one-fifth of their nominal capacity. For P. cepacia the medium consisted of inorganic salts solution (20) supplemented with 1% (wt/vol) Casamino Acids (Difco Laboratories, Detroit, Mich.). Strain SK1592 was grown in LB medium supplemented with either ampicillin (25 pig/ml) or chloramphenicol (20 jig/ml) depending on the vector used and the insert location.Isolation of DNA. Plasmid DNA was isolated by the procedure of Currier and Nester (12). When used as probes in Southern hybridization experiments the samples were subjected to two rounds of cesium chloride centrifugation. In some experiments plasmid DNA from E. coli Was isolated by the procedure of Bimboim and Doly (6

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Insertion-sequence-dependent rearrangements of Pseudomonas cepacia plasmid pTGL1

Gaffney¹,

Lessie²

1987

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…cepacia can degrade a wide variety of compounds as carbon and energy sources [13], including a variety of aromatic compounds [14,15] pesticides and herbicides [16]. It has the ability to degrade benzo(a) pyrene and other fused ring compounds [17], and biodegradation of heavy crude oil [18].…”

Section: Introductionmentioning

confidence: 99%

An Indigenous Biosurfactant Producing Burkholderia cepacia with High Emulsification Potential towards Crude Oil

Almatawah¹

2017

J Environ Anal Toxicol

View full text Add to dashboard Cite

“…strains are efficient biodegraders of toxic chemicals (5,20,25,43,47) as well as very effective in controlling plant pathogenic soil fungi and nematodes (11,13). Thus, they have been proposed for environmental release for purposes of toxic chemical bioremediation and enhanced agricultural productivity.…”

mentioning

confidence: 99%

Energy-Generating Enzymes ofBurkholderia cepaciaand Their Interactions with Macrophages

et al. 2003

View full text Add to dashboard Cite

We previously demonstrated that several clinical and environmental isolates of Burkholderia cepacia secreted ATP-utilizing enzymes to the medium; the secretion of these enzymes by cystic fibrosis lung isolate strain 38 was shown to be greatly enhanced in the presence of ␣ 2 -macroglobulin. Fractionation of the growth medium of cystic fibrosis isolate strain 71 belonging to genomovar I demonstrated the presence of two additional proteins, homologues of Pseudomonas aeruginosa azurin and cytochrome c 551 , which are normally involved in electron transfer during denitrification. A Q-Sepharose column flowthrough fraction of the growth medium of B. cepacia strain 71 enriched with the azurin and cytochrome c 551 homologues triggered apoptosis in macrophages and mast cells, leading to their death. Incubation of the Q-Sepharose column flowthrough fraction with antiazurin and anti-cytochrome c 551 antibodies greatly reduced cell death. We cloned and hyperexpressed a gene from B. cepacia strain 71 that encodes the homologue of P. aeruginosa azurin. Such azurin homologues were detected in the growth medium of several strains belonging to genomovars I, III, and VI but not in the growth medium of strains belonging to other genomovars. The growth medium of the strains that elaborated the azurin homologue had high cytotoxicity towards macrophages. Purified azurin homologue was shown to induce apoptosis in macrophages in a caspase-dependent manner and was localized in both the cytosol and nucleus when incubated with or microinjected into macrophages. This is an interesting example of the interaction of a bacterial protein normally involved in cellular energetics with macrophages to effect their cell death.

show abstract

Catabolic Potential of Pseudomonas cepacia

Cited by 43 publications

References 72 publications

Insertion-sequence-dependent rearrangements of Pseudomonas cepacia plasmid pTGL1

Insertion-sequence-dependent rearrangements of Pseudomonas cepacia plasmid pTGL1

An Indigenous Biosurfactant Producing Burkholderia cepacia with High Emulsification Potential towards Crude Oil

Energy-Generating Enzymes ofBurkholderia cepaciaand Their Interactions with Macrophages

Contact Info

Product

Resources

About