Caspases: Enemies Within

Thornberry, Nancy A.; Lazebnik, Yuri

doi:10.1126/science.281.5381.1312

Cited by 6,356 publications

(4,369 citation statements)

References 42 publications

Supporting

Mentioning

4,159

Contrasting

Unclassified

Order By: Relevance

“…Hallmarks of apoptosis include activation of caspases and mitochondrial membrane perturbation (Green and Reed, 1998;Thornberry and Lazebnik, 1998). These processes are, in part, regulated by members of the Bcl-2 family of cell death factors, some of which are protective and others causative (Green and Reed, 1998;Thornberry and Lazebnik, 1998).…”

Section: Coordinate Regulation Of Cell Cycle Withdrawal and Apoptosismentioning

confidence: 99%

“…These processes are, in part, regulated by members of the Bcl-2 family of cell death factors, some of which are protective and others causative (Green and Reed, 1998;Thornberry and Lazebnik, 1998). The ratio between pro-apoptotic and anti-apoptotic Bcl-2 factors has been shown to modulate the sensitivity of cells to mitochondrial disintegration in response to apoptotic signals (Oltvai et al, 1993;Gross et al, 1999).…”

Section: Coordinate Regulation Of Cell Cycle Withdrawal and Apoptosismentioning

confidence: 99%

See 1 more Smart Citation

Genome‐wide examination of myoblast cell cycle withdrawal during differentiation

Shen

Collier

Hlaing

et al. 2002

Developmental Dynamics

105

View full text Add to dashboard Cite

Skeletal and cardiac myocytes cease division within weeks of birth. Although skeletal muscle retains limited capacity for regeneration through recruitment of satellite cells, resident populations of adult myocardial stem cells have not been identified. Because cell cycle withdrawal accompanies myocyte differentiation, we hypothesized that C2C12 cells, a mouse myoblast cell line previously used to characterize myocyte differentiation, also would provide a model for studying cell cycle withdrawal during differentiation. C2C12 cells were differentiated in culture medium containing horse serum and harvested at various time points to characterize the expression profiles of known cell cycle and myogenic regulatory factors by immunoblot analysis. BrdU incorporation decreased dramatically in confluent cultures 48 hr after addition of horse serum, as cells started to form myotubes. This finding was preceded by up-regulation of MyoD, followed by myogenin, and activation of Bcl-2. Cyclin D1 was expressed in proliferating cultures and became undetectable in cultures containing 40% fused myotubes, as levels of p21 WAF1/Cip1 increased and ␣-actin became detectable. Because C2C12 myoblasts withdraw from the cell cycle during myocyte differentiation following a course that recapitulates this process in vivo, we performed a genome-wide screen to identify other gene products involved in this process. Using microarrays containing ϳ10,000 minimally redundant mouse sequences that map to the UniGene database of the National Center for Biotechnology Information, we compared gene expression profiles between proliferating, differentiating, and differentiated C2C12 cells and verified candidate genes demonstrating differential expression by RT-PCR. Cluster analysis of differentially expressed genes revealed groups of gene products involved in cell cycle withdrawal, muscle differentiation, and apoptosis. In addition, we identified several genes, including DDAH2 and Ly-6A, whose expression specifically was up-regulated during cell cycle withdrawal coincident with early myoblast differentiation. Developmental Dynamics 226:128 -138, 2003.

show abstract

Section: Coordinate Regulation Of Cell Cycle Withdrawal and Apoptosismentioning

confidence: 99%

Section: Coordinate Regulation Of Cell Cycle Withdrawal and Apoptosismentioning

confidence: 99%

Genome‐wide examination of myoblast cell cycle withdrawal during differentiation

Shen

Collier

Hlaing

et al. 2002

Developmental Dynamics

105

View full text Add to dashboard Cite

show abstract

“…Firstly, cell number was measured by nuclear counting. Secondly an antibody against caspase 3 (which is common to both the extrinsic and intrinsic apoptotic pathways),38 was used to identify cells which may be apoptotic. The third endpoint assessed was autophagy, a self‐digestion process that cells in stressed conditions undergo as part of their normal life cycle,39 but which has been linked to apoptosis 40.…”

mentioning

confidence: 99%

Selective and Potent Proteomimetic Inhibitors of Intracellular Protein–Protein Interactions

et al. 2015

View full text Add to dashboard Cite

Inhibition of protein–protein interactions (PPIs) represents a major challenge in chemical biology and drug discovery. α‐Helix mediated PPIs may be amenable to modulation using generic chemotypes, termed “proteomimetics”, which can be assembled in a modular manner to reproduce the vectoral presentation of key side chains found on a helical motif from one partner within the PPI. In this work, it is demonstrated that by using a library of N‐alkylated aromatic oligoamide helix mimetics, potent helix mimetics which reproduce their biophysical binding selectivity in a cellular context can be identified.

show abstract

“…TEM, considered as the ‘gold standard’ assay to confirm the cell death mechanism 29, was also performed. Alterations in the cell ultrastructure were observed including condensed chromatin, nuclear membrane disintegration, nuclear fragmentation, and plasma membrane blebbing, some well‐known features of apoptosis 28, 30, 31. The human melanoma cell line A2058 was equally affected by AC 1001 H3 at the same cytotoxic concentration showing condensed chromatin and a positive TUNEL assay for DNA degradation.…”

Section: Discussionmentioning

confidence: 99%

AC‐1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model

et al. 2016

View full text Add to dashboard Cite

Antibody‐derived peptides modulate functions of the immune system and are a source of anti‐infective and antitumor substances. Recent studies have shown that they comprise amino acid sequences of immunoglobulin complementarity‐determining regions, but also fragments of constant regions. VH CDR3 of murine mAb AC‐1001 displays antimetastatic activities using B16F10‐Nex2 murine melanoma cells in a syngeneic model. The peptide was cytotoxic in vitro in murine and human melanoma cells inducing reactive oxygen species (ROS) and apoptosis by the intrinsic pathway. Signs of autophagy were also suggested by the increased expression of LC3/LC3II and Beclin 1 and by ultrastructural evidence. AC‐1001 H3 bound to both G‐ and F‐actin and inhibited tumor cell migration. These results are important evidence of the antitumor activity of Ig CDR‐derived peptides.

show abstract

Caspases: Enemies Within

Cited by 6,356 publications

References 42 publications

Genome‐wide examination of myoblast cell cycle withdrawal during differentiation

Genome‐wide examination of myoblast cell cycle withdrawal during differentiation

Selective and Potent Proteomimetic Inhibitors of Intracellular Protein–Protein Interactions

AC‐1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model

Contact Info

Product

Resources

About