Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease

Abstract

BackgroundThe CARD family plays an important role in innate immune response by the activation of NF-κB. The aim of this study was to determine the gene expression and to enumerate the protein-expressing cells of some members of the CARD family (CARD9, CARD10, CARD11, CARD14 and CARD15) in patients with IBD and normal controls without colonic inflammation.MethodsWe included 48 UC patients, 10 Crohn’s disease (CD) patients and 18 non-inflamed controls. Gene expression was performed by RT-PCR and protein expression by immunohistochemistry. CARD-expressing cells were assessed by estimating the positively staining cells and reported as the percentage.ResultsThe CARD9 and CARD10 gene expression was significantly higher in UC groups compared with CD (P < 0.001). CARD11 had lower gene expression in UC than in CD patients (P < 0.001). CARD14 gene expression was higher in the group with active UC compared to non-inflamed controls (P < 0.001). The low expression of CARD14 gene was associated with a benign clinical course of UC, characterized by initial activity followed by long-term remission longer than 5 years (P = 0.01, OR = 0.07, 95%CI:0.007–0.70). CARD15 gene expression was lower in UC patients versus CD (P = 0.004). CARD9 protein expression was detected in inflammatory infiltrates; CARD14 in parenchymal cells, while CARD15 in inflammatory and parenchymal cells. CARD9−, CARD14− and CARD15 − expressing cells were significantly higher in patients with active UC versus non-inflamed controls (P < 0.05).ConclusionThe CARD family is involved in the inflammatory process and might be involved in the IBD pathophysiology.

CARMA3/NF-κB signaling contributes to tumorigenesis of hepatocellular carcinoma and is inhibited by sodium aescinate

“…CARMA3 is a member of the membrane-associated guanylate kinase (MAGUK) superfamily which acted as scaffold proteins. There are other two members in MAGUK family, CARMA1 and CARMA2[4]. All three proteins contained caspase recruitment domain, were known CARD11, CARD14 and CARD10, respectively[5].…”

Identification of novel key lncRNAs involved in periodontitis by weighted gene co‐expression network analysis

“…For example, we found that CARD10 was associated with inflammation by regulating NF‐κB activation and antiviral IFN signalling, and it was also reported to be involved in antiviral innate immune responses and DNA damage responses . In addition, CARD10 is critical for allergic airway inflammation and might be involved in IBD pathophysiology . The results suggest that CARD10 may play an important role in the progression of periodontitis inflammation.…”

“…37,38 In addition, CARD10 is critical for allergic airway inflammation and might be involved in IBD pathophysiology. 37,39 The results suggest that CARD10 may play an important role in the progression of periodontitis inflammation.…”

Case Report on Ulcerative Colitis in 16 year girl

“…The medication directly treats inflammation of the large intestine.rectal foam-a foamy substance the person puts into the rectum like an enema [17]‫۔‬The medication directly treats inflammation of the large intestine.,suppository-a solid medication the person inserts into the rectum to dissolve. The intestinal lining absorbs the medication [18].Some people will need surgery to treat their ulcerative colitis when they have:colon cancer,dysplasia, or precancerous cells in the colon,complications that are life threatening, such as megacolon or bleeding,no improvement in symptoms or condition despite treatment,continued dependency on steroids.side effects from medications that threaten their health [19].Removal of the entire colon, including the rectum, "cures" ulcerative colitis. A surgeon performs the procedure at a hospital [20] A surgeon can perform two different types of surgery to remove a patient's colon and treat ulcerative colitis:proctocolectomy and ileostomy,proctocolectomy and ileoanal reservoir,Full recovery from both operations may take 4 to 6 weeks.,Eating, Diet, and Nutrition.Researchers have not found that eating, diet, and nutrition play a role in causing ulcerative colitis symptoms.…”