2014
DOI: 10.1186/2051-5960-2-16
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Caspase-mediated cleavage of actin and tubulin is a common feature and sensitive marker of axonal degeneration in neural development and injury

Abstract: BackgroundAxon degeneration is a characteristic feature of multiple neuropathologic states and is also a mechanism of physiological neurodevelopmental pruning. The vast majority of in vivo studies looking at axon degeneration have relied on the use of classical silver degeneration stains, which have many limitations including lack of molecular specificity and incompatibility with immunolabeling methods. Because Wallerian degeneration is well known to involve cytoskeletal disassembly and because caspases are re… Show more

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Cited by 62 publications
(62 citation statements)
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References 39 publications
(65 reference statements)
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“…However, Casp6 causes axonal degeneration. Casp6 proteolytically cleaves cytoskeletal proteins crucial to neuronal integrity and function, such as alpha-tubulin, microtubule-associated protein Tau and post-synaptic density proteins regulating the actin cytoskeleton of the dendritic spines in synapses [29, 33, 56]. Casp6 is activated and associated with axonal degeneration in nerve growth factor-deprived mouse sensory neuron cultures, coordinately with cellular apoptosis generated by effector Casp3 activation [45, 52, 53, 64].…”
Section: Introductionmentioning
confidence: 99%
“…However, Casp6 causes axonal degeneration. Casp6 proteolytically cleaves cytoskeletal proteins crucial to neuronal integrity and function, such as alpha-tubulin, microtubule-associated protein Tau and post-synaptic density proteins regulating the actin cytoskeleton of the dendritic spines in synapses [29, 33, 56]. Casp6 is activated and associated with axonal degeneration in nerve growth factor-deprived mouse sensory neuron cultures, coordinately with cellular apoptosis generated by effector Casp3 activation [45, 52, 53, 64].…”
Section: Introductionmentioning
confidence: 99%
“…We observed mitochondrial deficits in the axon at 10 μg/ml cisplatin, which did lead to detectable active caspase 3 when we increased the concentration to 25 μg/ml. In mammalian neurons, active caspase 3 is observed in the axon of degenerating neurons (King et al, 2013; Simon et al, 2012; Sokolowski et al, 2014). It is possible that localized caspase 3 activation may occur in the axon.…”
Section: Discussionmentioning
confidence: 99%
“…Cytoskeletal disassembly and caspases are recently implicated in these processes highlighting the degenerating axonal processes in human hypoxic-ischaemic injury [29].…”
Section: Discussionmentioning
confidence: 99%