Caspase-1, -2, and -3 gene expression is enhanced in the heart and lung of chickens with pulmonary hypertension (ascites)

Hassanpour, Hossein; TESHFAM, Masood; Momtaz, Hassan; ZAREI, Hamed; BAHADORAN, Shahab

doi:10.3906/vet-1302-37

Cited by 4 publications

(11 citation statements)

References 22 publications

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“…The aim of this study was to investigate the effect of three antioxidant agents including vitamins C and E and garlic powder on blood cell analysis, heterophil, lymphocyte, and heterophil/lymphocyte ratio as well as lipid peroxidation levels in broiler chicken with PHS syndrome induced by T3 administration at three interval times of age. The increased RV/TV ra-tio more than 0.229 in the control group confirmed induction of PHS (Hassanpour et al, 2014).…”

Section: Discussionmentioning

confidence: 60%

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Natural antioxidants improve pulmonary hypertension syndrome and change blood profile in broiler chicken

Zarei

Moghani

2022

J Hellenic Vet Med Soc

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The objective of the present study was to investigate the effect of vitamins C and E and garlic powder on hetrophil/lymphocyte and right ventricle (RV)/total ventricle (TV) ratio as well as lipid peroxidation levels in BC with PHS induced by T3 administration. A total of 225 chickens (Ross 308, 1 day old) were randomly divided into five equal groups (n=45) including the control (received standard basal diet (RSBD)+1.5 mg/kg of T3), sham (RSBD over period study), and the vitamin C (RSBD+1200 PPM of vitamin C+1.5 mg/kg of T3), the vitamin E (RSBD+400 mg/kg of vitamin E+1.5 mg/kg of T3), the garlic (RSBD +1% garlic+1.5mg/kg of T3) groups. T3 was administrated at days 7 to 49 of age. At days 21,35 and 49 post- reared, 15 chicks from each group were randomly selected, scarified and blood parameters including lymphocytes, heterophil, hetrophil/lymphocyte and RV/TV ratio as well as lipid peroxidation were assayed. The decreased the RV/TV ratio in the all treatment groups compared to control group indicate that PHS-induced by administration of T3 improve by adding of vitamins E, vitamin C and garlic to basal diet. The highest and lowest mean percentage of heterophil was belonged to the control and sham groups on 35 and 49 days of age, respectively. The highest mean percentage of heterophil/lymphocyte ratio was belonged to the control groups on days of 35, and 49 of age. The adding of vitamin C and garlic to basal diet resulted in a significant increase in the mean percentage of lymphocyte compared to the control and the vitamin E groups on days 35 and 49 of age. In conclusion the natural antioxidants such as garlic, vitamins E and C can improve the increased RV/TV and heterophil/lymphocyte ratios in the blood profile of broiler chickens with PHS due to reduce lipid peroxidation.

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Section: Discussionmentioning

confidence: 60%

“…At days 21, 35 and 49 of age the chicks were sacrificed and RVH was then determined as previously described by Hassanpour et al, (2014). The hearts were resected and total ventricles were weighted.…”

Section: Assessment Of Cardiac Parametermentioning

confidence: 99%

Natural antioxidants improve pulmonary hypertension syndrome and change blood profile in broiler chicken

Zarei

Moghani

2022

J Hellenic Vet Med Soc

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“…It has been shown that exposure of broiler chickens to oxidative stress increases the apoptosis of splenocytes due to the up-regulation of the expression of caspase-3 (CASP3) and caspase-9 (CASP9) genes, as well as impaired immune function (Oloruntola et al, 2023;Chen et al, 2019). In addition, other apoptosis genes, such as caspase-1 (CASP1), caspase-2 (CASP2), and CASP3, were up-regulated in the hearts and lungs of chickens with PHS (Hassanpour et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Vitamin E Can Down-regulate Some of Apoptotic Genes Involved in Pulmonary Hypertension Syndrome in Broiler Chicken

Zarei,

Gilvari

2024

IJVM

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Background: Ascites or pulmonary hypertension syndrome (PHS) is one of the significant problems in the poultry industry. Therefore, various studies have been conducted on its contributing factors. Objectives: This study aimed to investigate the role of vitamin E in reducing the mRNA levels of caspase-1 (CASP1), caspase-2 (CASP2), and caspase-3 (CASP3) genes involved in the apoptosis pathway. Methods: Ninety fast-growing 1-day-old chickens (Ross 308) were randomly assigned to three equal groups, including sham (basal diet), control (basal diet+1.5 mg/kg of triiodothyronine [T3]), and treatment group (basal diet+400 mg/kg of vitamin E+1.5 mg/kg of T3). To induce ascites, 1.5 mg/kg of T3 was added to basal diet from the seventh day to the end of the experiment. On the 21st and 49th days after rearing, 15 chicks from each group were randomly selected. The right ventricle/total ventricle weight ratio (RV/TV) and the expression levels of CASP1, CASP2, and CASP3 genes in the lung and right ventricle of all three groups of broiler chickens were measured and compared. Results: Although there was no significant difference between the three groups in terms of the RV/TV ratio on day 21 post-reared (P≥0.05), a significant decrease was detected in the vitamin E-receiving group compared to the control group with respect to the RV/TV ratio on day 49 post-reared (P<0.05). Also, vitamin E reduced the relative expression of CASP1, CASP2, and CASP3 at 49 days of age in the lung and heart tissues of broiler chickens with ascites (P<0.05). Conclusion: Based on the results of this study, it seems that vitamin E can reduce some apoptosis genes (CASP1, CASP2, and CASP3) associated with pulmonary hypertension in broilers.

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“…However, an overexpression of Hsp 70 is seen in tumor cells and Hsp 70 inhibits apoptosis in both intrinsic and extrinsic pathways. Thereby, Hsp 70 has recently become a marker for prognosis and a pharmological target which its inhibitors have been researched [8][9][10][11][12][13][14][15][16][17][18][19][20][21].…”

Section: Ht-29 and Cf-1 (Mef) Cell Culture And The Treatment Of The Hmentioning

confidence: 99%

“…Two major apoptosis signaling pathway exist in cells, namely: i) intrinsic which occurs in mitochondria and ii) extrinsic pathway. The latter is induced by death receptors which belong to TNF (Tumor Necrosis Factor) receptor super family such as TNF-α, DR 4, DR 5, Fas (CD 95) [9,10] and afterwards induces activity of caspase 8 and caspase 3 which are initiator and effector caspases, recpectively [11,12]. However, stimuli such as anticancer drugs trigger intrinsic pathway through differentiation of the mitochondrial permeability.…”

Section: Introductionmentioning

confidence: 99%

Anticancer activity of the “Hardaliye” on HT-29 Cell Line and proliferative activity on CF-1 cell line: Apoptosis and Antioxidant pathway responsive gene expressions

Kahraman-Ilıkkan¹,

Doğanlar²,

Doğanlar³

et al. 2017

Integr Mol Med

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The study was designed to detect anticancer activity and proliferative effect of the Hardaliye in HT-29 and CF-1 cell lines, respectively. Based on MTT results, two dilutions namely, five (H5) and ten-fold (H10) were selected for further analyses. Apoptosis and antioxidant pathway related gene expression levels were detected using qRT-PCR and tali apoptosis assay was used to screen percentage levels of death, apoptotic and viable cells after 24h and 48h of treatment.Results suggested that Hardaliye was effective on cancer cells and trigger apoptosis via increase of Bax (22.45 ± 3.1-fold for H5 and 6.49 ± 0.1-fold for H10) as well as SOD and CAT activity. However, Hardaliye also affected slightly healthy cells when diluted fivefold, though, did not affect when dilute tenfold. According to gene expression levels, an increase at antioxidant enzyme and apoptosis inhibitors was seen after 24 h. On the other hand, antioxidant enzymes apart from GpX of H5, decreased after 48h. Predictably, antioxidant substances of Hardaliye revealed an antioxidant activity over time.Overall, this was the first report considering anticancer activity of Hardaliye besides its efficacy on healthy cells. In this respect, these preliminary results can indicate potential of Hardaliye as a food support in preventing colon cancer.

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Caspase-1, -2, and -3 gene expression is enhanced in the heart and lung of chickens with pulmonary hypertension (ascites)

Cited by 4 publications

References 22 publications

Natural antioxidants improve pulmonary hypertension syndrome and change blood profile in broiler chicken

Natural antioxidants improve pulmonary hypertension syndrome and change blood profile in broiler chicken

Vitamin E Can Down-regulate Some of Apoptotic Genes Involved in Pulmonary Hypertension Syndrome in Broiler Chicken

Anticancer activity of the “Hardaliye” on HT-29 Cell Line and proliferative activity on CF-1 cell line: Apoptosis and Antioxidant pathway responsive gene expressions

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