Casein Kinase 1δ Stabilizes Mature Axons by Inhibiting Transcription Termination of Ankyrin

LaBella, Matthew L.; Hujber, Edward J.; Moore, Kristin Anderson; Rawson, Randi L.; Merrill, Sean; Allaire, Patrick D.; Ailion, Michael; Hollien, Julie; Bastiani, Michael J.; Jørgensen, Erik M.

doi:10.1016/j.devcel.2019.12.005

Cited by 16 publications

(25 citation statements)

References 114 publications

(144 reference statements)

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“…However, CK1δ mutations lead to continuous elongation of their growth cones after the late larval L1 stage, which leads to a highly branched nervous system (LaBella et al, 2020). The overgrowth phenotype can be suppressed by expression of the giant isoform of Ankyrin, or mutations in the RNA polymerase-II termination complex (LaBella et al, 2020). 3 ′ UTR choice in the mRNA coding for the Drosophila Dscam1 cell-surface receptor provides another compelling example of the role of APA in axon morphogenesis.…”

Section: Alternative Cleavage and Polyadenylationmentioning

confidence: 99%

“…Conversely, toward the end of axon morphogenesis, selection of the most distal PAS in a component of the cytoskeleton, Ankyrin, mediates stabilization of mature axons and synapses, and growth arrest (Knobel et al, 2001 ; Pielage et al, 2008 ). In Caenorhabditis elegans , at the end of axon morphogenesis, the casein kinase 1δ (CK1δ) localizes to the nucleus and inhibits transcription termination of ankyrin , leading to the production of the longer isoform (LaBella et al, 2020 ). CK1δ regulates APA by phosphorylating several components of the RNA polymerase-II termination complex (LaBella et al, 2020 ).…”

Section: Roles Of Rna Metabolism In Neuronal Wiring and Remodelingmentioning

confidence: 99%

“…In Caenorhabditis elegans , at the end of axon morphogenesis, the casein kinase 1δ (CK1δ) localizes to the nucleus and inhibits transcription termination of ankyrin , leading to the production of the longer isoform (LaBella et al, 2020 ). CK1δ regulates APA by phosphorylating several components of the RNA polymerase-II termination complex (LaBella et al, 2020 ). Gamma-aminobutyric acid (GABA) motor neurons extend axons from the ventral nerve cord to the dorsal nerve cord during a specific developmental window, after which axon outgrowth stops.…”

Section: Roles Of Rna Metabolism In Neuronal Wiring and Remodelingmentioning

confidence: 99%

“…Gamma-aminobutyric acid (GABA) motor neurons extend axons from the ventral nerve cord to the dorsal nerve cord during a specific developmental window, after which axon outgrowth stops. Mutations in CK1δ do not affect axon growth, branching, or synaptogenesis during development of GABA motor neurons (LaBella et al, 2020 ). However, CK1δ mutations lead to continuous elongation of their growth cones after the late larval L1 stage, which leads to a highly branched nervous system (LaBella et al, 2020 ).…”

Section: Roles Of Rna Metabolism In Neuronal Wiring and Remodelingmentioning

confidence: 99%

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The Fine Art of Writing a Message: RNA Metabolism in the Shaping and Remodeling of the Nervous System

Landínez-Macías

Urwyler

2021

Front. Mol. Neurosci.

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Neuronal morphogenesis, integration into circuits, and remodeling of synaptic connections occur in temporally and spatially defined steps. Accordingly, the expression of proteins and specific protein isoforms that contribute to these processes must be controlled quantitatively in time and space. A wide variety of post-transcriptional regulatory mechanisms, which act on pre-mRNA and mRNA molecules contribute to this control. They are thereby critically involved in physiological and pathophysiological nervous system development, function, and maintenance. Here, we review recent findings on how mRNA metabolism contributes to neuronal development, from neural stem cell maintenance to synapse specification, with a particular focus on axon growth, guidance, branching, and synapse formation. We emphasize the role of RNA-binding proteins, and highlight their emerging roles in the poorly understood molecular processes of RNA editing, alternative polyadenylation, and temporal control of splicing, while also discussing alternative splicing, RNA localization, and local translation. We illustrate with the example of the evolutionary conserved Musashi protein family how individual RNA-binding proteins are, on the one hand, acting in different processes of RNA metabolism, and, on the other hand, impacting multiple steps in neuronal development and circuit formation. Finally, we provide links to diseases that have been associated with the malfunction of RNA-binding proteins and disrupted post-transcriptional regulation.

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Section: Alternative Cleavage and Polyadenylationmentioning

confidence: 99%

Section: Roles Of Rna Metabolism In Neuronal Wiring and Remodelingmentioning

confidence: 99%

Section: Roles Of Rna Metabolism In Neuronal Wiring and Remodelingmentioning

confidence: 99%

Section: Roles Of Rna Metabolism In Neuronal Wiring and Remodelingmentioning

confidence: 99%

See 2 more Smart Citations

The Fine Art of Writing a Message: RNA Metabolism in the Shaping and Remodeling of the Nervous System

Landínez-Macías

Urwyler

2021

Front. Mol. Neurosci.

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“…For example, JAK kinase is involved in IL2 synthesis [57], thyrotropin signaling [58], and centrosomal protein phosphorylation [59]. Similarly, vertebrate casein kinase 1δ stabilizes mature axons, [60], governs periodicity of mammalian circadian rhythms [61], and regulates cognitive-affective behavior in mice [62]. Protein kinases exhibit this myriad of functions because they have multiple substrates, some of which are effectors for pathways regulated by the enzymes [56].…”

Section: Casein Kinase Tbck12 Has Multiple Functions In the African mentioning

confidence: 99%

Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome

et al. 2021

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The single mitochondrial nucleoid (kinetoplast) of Trypanosoma brucei is found proximal to a basal body (mature (mBB)/probasal body (pBB) pair). Kinetoplast inheritance requires synthesis of, and scission of kinetoplast DNA (kDNA) generating two kinetoplasts that segregate with basal bodies into daughter cells. Molecular details of kinetoplast scission and the extent to which basal body separation influences the process are unavailable. To address this topic, we followed basal body movements in bloodstream trypanosomes following depletion of protein kinase TbCK1.2 which promotes kinetoplast division. In control cells we found that pBBs are positioned 0.4 um from mBBs in G1, and they mature after separating from mBBs by at least 0.8 um: mBB separation reaches ~2.2 um. These data indicate that current models of basal body biogenesis in which pBBs mature in close proximity to mBBs may need to be revisited. Knockdown of TbCK1.2 produced trypanosomes containing one kinetoplast and two nuclei (1K2N), increased the percentage of cells with uncleaved kDNA 400%, decreased mBB spacing by 15%, and inhibited cytokinesis 300%. We conclude that (a) separation of mBBs beyond a threshold of 1.8 um correlates with division of kDNA, and (b) TbCK1.2 regulates kDNA scission. We propose a Kinetoplast Division Factor hypothesis that integrates these data into a pathway for biogenesis of two daughter mitochondrial nucleoids.

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Poly(A) tale: From A to A; RNA polyadenylation in prokaryotes and eukaryotes

Mofayezi,

Jadaliha,

Zangeneh

et al. 2024

WIREs RNA

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Most eukaryotic mRNAs and different non‐coding RNAs undergo a form of 3′ end processing known as polyadenylation. Polyadenylation machinery is present in almost all organisms except few species. In bacteria, the machinery has evolved from PNPase, which adds heteropolymeric tails, to a poly(A)‐specific polymerase. Differently, a complex machinery for accurate polyadenylation and several non‐canonical poly(A) polymerases are developed in eukaryotes. The role of poly(A) tail has also evolved from serving as a degradative signal to a stabilizing modification that also regulates translation. In this review, we discuss poly(A) tail emergence in prokaryotes and its development into a stable, yet dynamic feature at the 3′ end of mRNAs in eukaryotes. We also describe how appearance of novel poly(A) polymerases gives cells flexibility to shape poly(A) tail. We explain how poly(A) tail dynamics help regulate cognate RNA metabolism in a context‐dependent manner, such as during oocyte maturation. Finally, we describe specific mRNAs in metazoans that bear stem‐loops instead of poly(A) tails. We conclude with how recent discoveries about poly(A) tail can be applied to mRNA technology.This article is categorized under: RNA Evolution and Genomics > RNA and Ribonucleoprotein Evolution RNA Processing > 3′ End Processing RNA Turnover and Surveillance > Regulation of RNA Stability

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Casein Kinase 1δ Stabilizes Mature Axons by Inhibiting Transcription Termination of Ankyrin

Cited by 16 publications

References 114 publications

The Fine Art of Writing a Message: RNA Metabolism in the Shaping and Remodeling of the Nervous System

The Fine Art of Writing a Message: RNA Metabolism in the Shaping and Remodeling of the Nervous System

Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome

Poly(A) tale: From A to A; RNA polyadenylation in prokaryotes and eukaryotes

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