Case study of the morphologic variation of circulating tumor cells

Marrinucci, Dena; Bethel, Kelly; Bruce, Richard H.; Curry, Douglas N.; Hsieh, B.J.; Humphrey, Mark; Krivacic, Robert T.; Kroener, Joan F.; Kroener, Lindsay; Ladányi, András; Lazarus, Nicole H.; Nieva, Jorgé; Kühn, Peter

doi:10.1016/j.humpath.2006.08.027

Cited by 143 publications

(140 citation statements)

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“…That there were no differences in the morphology of CTCs in relation with the grade of differentiation of the primary or metastatic tumor, or between primary and secondary CTCs and that in general all were of a more rounded more suggests that in the blood environment without the presence of tissue support the cells take on a more spherical form rather than a columnar form. This has been documented in other tumors where CTCs tended to rounded or oval in shape (Molnar et al;Marrinucci et al, 2007).…”

Section: Discussionsupporting

confidence: 58%

Cytomorphology of Circulating Tumor Cells and Cell Clusters in Chilean Patients with Colo-Rectal Cancer

Murray

Albarran

et al. 2012

Int. J. Morphol.

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SUMMARY:The aim of this study was to determine the cytomorphological characteristics of circulating tumor cells (CTCS) in patients with colo-rectal cancer and compare them with the primary tumor and metastasis. CTCS were obtained from blood using differential gel centrifugation and detected using standard immunocytochemistry using anti-CEA. Primary CTCs were defined as those detected before surgery and secondary CTCs those detected after. Surgical specimens of the primary tumor and metastasis were evaluated using standard histological methods with hematoxillin and eosin. CTCs both primary and secondary retained the cytomorphological characteristics of the primary tumor, showing marked intra-patient pleomorphism. There were no differences between primary and secondary CTCs in their cytomorphological features. CTCs from patients with signet ring tumors showed the presence of intracellular mucin deposits. Groups of 3 or more CTCs were only seen in patients with metastasis, whereas duplets of CTcs were seen in patients with metastatic and non-metastatic colo-rectal cancer. This study provides an initial analysis of the cytomorphological features of CTCs, providing a foundation for further investigation into the significance and metastatic potential of CTCs.

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Section: Discussionsupporting

confidence: 58%

Cytomorphology of Circulating Tumor Cells and Cell Clusters in Chilean Patients with Colo-Rectal Cancer

Murray

Albarran

et al. 2012

Int. J. Morphol.

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“…Thus, wild-type or mutant genes related to cell cycle can overexpress, and as a result, the S phase will be completed in the cell cycle and the cell genetic content will increase. This phenomenon can explain the cause of increased nuclear pleomorphism (Marrinucci et al, 2007;Kerbel, 2008). Another finding of our study indicates the lack of correlation between MVD and the mitotic count in breast cancer.…”

Section: Discussionmentioning

confidence: 46%

Correlation of Microvessel Density with Nuclear Pleomorphism, Mitotic Count and Vascular Invasion in Breast and Prostate Cancers at Preclinical and Clinical Levels

Muhammadnejad

Muhammadnejad²,

Haddadi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Tumor angiogenesis correlates with recurrence and appears to be a prognostic factor for both breast and prostate cancers. In the present study, we aimed to investigate the correlation of microvessel density (MVD), a measure of angiogenesis, with nuclear pleomorphism, mitotic count, and vascular invasion in breast and prostate cancers at preclinical and clinical levels. Methods: Samples from xenograft tumors of luminal B breast cancer and prostate adenocarcinoma, established by BT-474 and PC-3 cell lines, respectively, and commensurate human paraffin-embedded blocks were obtained. To determine MVD, specimens were immunostained for CD-34. Nuclear pleomorphism, mitotic count, and vascular invasion were determined using hematoxylin and eosin (H&E)-stained slides. Results: MVD showed significant correlations with nuclear pleomorphism (r=0.68, P=0.03) and vascular invasion (r=0.77, P=0.009) in breast cancer. In prostate cancer, MVD was significantly correlated with nuclear pleomorphism (r=0.75, P=0.013) and mitotic count (r=0.75, P=0.012). In the breast cancer xenograft model, a significant correlation was observed between MVD and vascular invasion (r=0.87, P=0.011). In the prostate cancer xenograft model, MVD was significantly correlated with all three parameters (nuclear pleomorphism, r=0.95, P=0.001; mitotic count, r=0.91, P=0.001; and vascular invasion, r=0.79, P=0.017; respectively). Conclusions: Our results demonstrate that MVD is correlated with nuclear pleomorphism, mitotic count, and vascular invasion at both preclinical and clinical levels. This study therefore supports the predictive value of MVD in breast and prostate cancers.

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“…Measurements of the nuclear to cytoplasmic ratio (N/C), which may allow one to infer relative deformability, indicate that CTCs are less deformable than leukocytes; Meng et al reported average N/ C ratios of 0.8 and 0.55 for CTCs and leukocytes, respectively. Similar to size and other morphological features of CTCs, several studies have reported significant variation in N/C (Marrinucci et al, 2007(Marrinucci et al, , 2010. Interestingly, these differences may function as a biomarker to identify more aggressive tumors, as CTCs isolated from castrate-resistant prostate cancer patients were approximately three times more deformable than castrate-sensitive samples as measured by atomic force microscopy (Osmulski et al, 2014).…”

Section: Deformability Of Tumor Cellsmentioning

confidence: 89%

Circulating tumor cell technologies

2016

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Cancer CTC capture CTC clustersImmunoaffinity enrichment A B S T R A C TCirculating tumor cells, a component of the "liquid biopsy", hold great potential to transform the current landscape of cancer therapy. A key challenge to unlocking the clinical utility of CTCs lies in the ability to detect and isolate these rare cells using methods amenable to downstream characterization and other applications. In this review, we will provide an overview of current technologies used to detect and capture CTCs with brief insights into the workings of individual technologies. We focus on the strategies employed by different platforms and discuss the advantages of each. As our understanding of CTC biology matures, CTC technologies will need to evolve, and we discuss some of the present challenges facing the field in light of recent data encompassing epithelial-to-mesenchymal transition, tumor-initiating cells, and CTC clusters.

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Case study of the morphologic variation of circulating tumor cells

Cited by 143 publications

References 13 publications

Cytomorphology of Circulating Tumor Cells and Cell Clusters in Chilean Patients with Colo-Rectal Cancer

Cytomorphology of Circulating Tumor Cells and Cell Clusters in Chilean Patients with Colo-Rectal Cancer

Correlation of Microvessel Density with Nuclear Pleomorphism, Mitotic Count and Vascular Invasion in Breast and Prostate Cancers at Preclinical and Clinical Levels

Circulating tumor cell technologies

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