Case report: successful pregnancy after vitrification of a human blastocyst that had completely escaped from the zona pellucida on day 6

Hiraoka, Kenichiro

doi:10.1093/humrep/deh177

Cited by 27 publications

(16 citation statements)

References 12 publications

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“…Several studies on mammalian embryos have proven that the best tool for vitrification is cryotop [28,[34][35][36]. In the Zhang study [36], the vitrified mouse embryos derived in vivo with cryotop showed survival rates of 96 % for two-cell vitrified embryos.…”

Section: Discussionmentioning

confidence: 99%

Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

Dehghani-Mohammadabadi

Salehi

Farifteh

et al. 2014

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

Dehghani-Mohammadabadi

Salehi

Farifteh

et al. 2014

J Assist Reprod Genet

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“…With the improvement of culture systems and blastocyst cryopreservation techniques, some pregnancies are achieved following the transfer of cryopreserved day-7 zona-intact blastocysts [13][14][15], re-cryopreserved day-7 zona intact blastocysts [16], and day-5 and day-6 spontaneously hatched blastocysts [17][18][19][20]. However, as far as is known, there are no reports on pregnancies following the transfer of re-cryopreserved day-7 spontaneously hatched blastocysts.…”

Section: Introductionmentioning

confidence: 99%

Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos

Hiraoka¹,

Horiuchi

Kusuda³

et al. 2009

J Assist Reprod Genet

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Purpose To report a successful delivery after the transfer of a re-cryopreserved day-7 hatched blastocyst. Methods and Results A 30-year-old woman underwent a long-treatment protocol for ovarian stimulation. Fourteen mature oocytes were obtained, and twelve were fertilized. On day 3, two cleaved embryos were transferred, but no implantation occurred. The remaining embryos were vitrified. Subsequently, two vitrified day-3 embryos were transferred. The woman became pregnant and delivered a healthy baby. Subsequently, two vitrified day-3 embryos were transferred, but no pregnancy occurred. Subsequently, all the remaining vitrified day-3 embryos were cultured. On day 5, no blastocyst was obtained. The remaining embryos were continued to be cultured. On day 7, a hatched blastocyst was obtained and revitrified. Subsequently, the re-vitrified day-7 hatched blastocyst was transferred. The woman became pregnant and delivered a healthy female. Conclusions The day-7 hatched blastocyst developed from vitrified embryos can be re-vitrified and have pregnancy potential after re-warming.

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“…Many research studies have been focused on vitrification due to significantly higher survival and pregnancy rates observed. The pregnancy rate and survival rate are reported as 37-48% and 70-95%, respectively [7,[21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocyte retrieval and IVF outcomes.

Kuć

Kuczyńska²,

Stankiewicz³

et al. 2010

Folia Histochem Cytobiol.

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Modifying cryopreservation protocols may be seen as a way to simplify cryobanking procedure and increase satisfying outcomes. The aim of the study was to evaluate the influence of slow cooling protocol and vitrification on IVF outcomes using embryos preserved in the 5 th or 6 th day after oocyte retrieval. The study compared 2 groups of human embryos underwent slow cooling protocol (n=189) and vitrification (n=58). All embryos were cryopreserved in the 5 th or 6 th day after oocyte retrieval. Pre-and postfreezing embryo evaluation was performed in 2 or 3 steps scale, respectively. The study evaluates the effectiveness of two freezing methods and influence of the freezing day, pre-and postfreezing embryo grading on clinical pregnancy rate. Study showed higher pregnancy rate after vitrification (50.4%) than slow cooling protocol (25.9%). Significantly higher pregnancy rate was observed, when embryo preserved in the 5 th day after oocyte retrieval (50.3%) than in the 6 th day (22.7%). Postfreezing embryos evaluation showed that high quality blastocysts gave nearly four times better pregnancy outcomes than the ones evaluated as poor quality, and three times better than the ones evaluated as moderate. Prospective trials are needed to evaluate pregnancy and neonatal outcomes after vitrification. The number of controlled studies concerning vitrification has not been large enough, yet.

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Case report: successful pregnancy after vitrification of a human blastocyst that had completely escaped from the zona pellucida on day 6

Cited by 27 publications

References 12 publications

Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos

Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocyte retrieval and IVF outcomes.

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