“…Previous studies identified E. cuniculi by PCR in an AIDS patient (genotype III) [ 35 ], Crohn’s disease patients [ 39 ], water samples from Madrid (genotypes I and III) [ 38 ], the Iberian lynx ( Lynx pardinus ) in southern Spain [ 40 ], and captive chimpanzees ( Pan troglodytes ) in Madrid (genotype I) [ 41 ], demonstrating the presence of this species in mainland Spain. Furthermore, other studies reported cases in domestic rabbits in Spain using indirect immunofluorescence testing [ 36 ], histopathological analysis [ 42 , 43 ], or serological analysis [ 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. However, the molecular characterization of E. cuniculi in rabbits from Spain has not been reported previously.…”