Cascade and Ultrafast Artificial Antioxidases Alleviate Inflammation and Bone Resorption in Periodontitis

Abstract

Periodontitis, one of the most common, challenging, and rapidly expanding oral diseases, is an oxidative stress-related disease caused by excessive reactive oxygen species (ROS) production. Developing ROS-scavenging materials to regulate the periodontium microenvironments is essential for treating periodontitis. Here, we report on creating cobalt oxide-supported Ir (CoO–Ir) as a cascade and ultrafast artificial antioxidase to alleviate local tissue inflammation and bone resorption in periodontitis. It is demonstrated that the Ir nanoclusters are uniformly supported on the CoO lattice, and there is stable chemical coupling and strong charge transfer from Co to Ir sites. Benefiting from its structural advantages, CoO–Ir presents cascade and ultrafast superoxide dismutase-catalase-like catalytic activities. Notably, it displays distinctly increased V max (76.249 mg L–1 min–1) and turnover number (2.736 s–1) when eliminating H2O2, which surpasses most of the by-far-reported artificial enzymes. Consequently, the CoO–Ir not only provides efficient cellular protection from ROS attack but also promotes osteogenetic differentiation in vitro. Furthermore, CoO–Ir can efficiently combat periodontitis by inhibiting inflammation-induced tissue destruction and promoting osteogenic regeneration. We believe that this report will shed meaningful light on creating cascade and ultrafast artificial antioxidases and offer an effective strategy to combat tissue inflammation and osteogenic resorption in oxidative stress-related diseases.

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

“…The positive control showed a large number of red nodules, indicating that calcium deposition was caused by osteogenic differentiation. The group treated with H 2 O 2 exhibited a reduced number of spots as a result of the suppression of ROS, which is consistent with previous reports . Meaningfully, this process was reversed in the H 2 O 2 +MT and H 2 O 2 +MT-CDs groups.…”

“…The alizarin red staining and its semiquantitative analysis further demonstrated that MT-CDs have a more significant advantage in promoting osteogenesis under ROS conditions than MT (Figure K). BMP-2 and Runx2 are essential for osteogenesis and accelerate the healing of bone defects. , BMP-2, a potent cytokine promoting predifferentiation of osteoblasts and bone development, plays a crucial osteogenic role . Additionally, Runx2 is an essential transcription factor that holds a pivotal function in the maturation of early stage osteoblasts .…”

Reactive oxygen nanobiocatalysts: activity-mechanism disclosures, catalytic center evolutions, and changing states

“…In addition, novel target-oriented characterisation techniques, especially in situ methods, such as in situ XAS, in situ XRD, and also in situ Raman, have emerged as frontier techniques to perform real-time monitors for a better understanding of the catalytic mechanisms, thus facilitating the DFT calculation, and guiding the future design of catalysts also deserves more attention to clearly detect the complex catalytic conversion chemistry and to reveal the catalytic mechanisms of RONBCs. 303–305…”

Photo-crosslinked integrated triphasic scaffolds with gradient composition and strength for osteochondral regeneration

“…35–38 However, they often face challenges with interface integration, as poor integration can result in scaffold delamination and failure of tissue regeneration. 19,39,40…”