Cas9-edited immune checkpoint blockade PD-1 DNA polyaptamer hydrogel for cancer immunotherapy

Lee, Jaiwoo; Le, Quoc‐Viet; Yang, Geon; Oh, Yu‐Kyoung

doi:10.1016/j.biomaterials.2019.119359

Cited by 68 publications

(52 citation statements)

References 36 publications

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“…These multivalent aptamers can also be incorporated with reduced graphene oxide, magnetic beads, FRET (fluorescent resonance energy transfer)‐based fluorescence molecules, or ferrocene (Figure C) for enhanced drug loading, efficient drug‐releasing or multiplexed cell imaging. Furthermore, RCA‐based multivalent aptamers could be incorporated with fluorophore‐labeled oligonucleotide or HRP for biosensors, used to prepare degradable aptamer hydrogel for cancer immunotherapy, or as an amplification strategy for aptamer SELEX . Owing to their enhanced binding affinity, size‐tunable property, exceptionally resistant ability to exonuclease degradation, and capability to circumvent multidrug resistance, multivalent aptamers from RCA have great potential for practical biomedical application.…”

Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

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acids (CNAs) are nucleic acid molecules with a closed-loop structure. This feature comes with a number of advantages including complete resistance to exonuclease degradation, much better thermodynamic stability, and the capability of being replicated by a DNA polymerase in a rolling circle manner. Circular functional nucleic acids, CNAs containing at least a ribozyme/DNAzyme or a DNA/RNA aptamer, not only inherit the advantages of CNAs but also offer some unique application opportunities, such as the design of topologycontrolled or enabled molecular devices. This article will begin by summarizing the discovery, biogenesis, and applications of naturally occurring CNAs, followed by discussing the methods for constructing artificial CNAs. The exploitation of circular functional nucleic acids for applications in nanodevice engineering, biosensing, and drug delivery will be reviewed next. Finally, the efforts to couple functional nucleic acids with rolling circle amplification for ultra-sensitive biosensing and for synthesizing multivalent molecular scaffolds for unique applications in biosensing and drug delivery will be recapitulated.[a] Dr.

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Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

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“…The release of a programmed death receptor (PD-1) DNA aptamer promotes the activation of T-cells and reduces tumor growth. 72 Furthermore, immune checkpoint inhibitors can be released with a hydrogel-based polypeptide vaccine to improve tumor immunotherapy. Song et al developed an injectable PEG-b-poly(L-alanine) hydrogel with encapsulated granulocyte-macrophage colony-stimulating factor and anti-CTLA-4/PD-1 antibodies.…”

Section: Studying the Immunological Response In Cancer Microenvironmentsmentioning

confidence: 99%

Emerging Biomimetic Materials for Studying Tumor and Immune Cell Behavior

2019

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Cancer is one of the leading causes of death both in the United States and worldwide. The dynamic microenvironment in which tumors grow consists of fibroblasts, immune cells, extracellular matrix (ECM), and cytokines that enable progression and metastasis. Novel biomaterials that mimic these complex surroundings give insight into the biological, chemical, and physical environment that cause cancer cells to metastasize and invade in to other tissues. Twodimensional (2D) cultures are useful for gaining limited information about cancer cell behavior; however, they do not accurately represent the environments that cells experience in vivo. Recent advances in the design and tunability of diverse three-dimensional (3D) biomaterials complement biological knowledge and allow for improved recapitulation of in vivo conditions. Understanding cell-ECM and cell-cell interactions that facilitate tumor survival will accelerate the design of more effective therapies. This review discusses innovative materials currently being used to study tumor and immune cell behavior and interactions, including materials that mimic the ECM composition, mechanical stiffness, and integrin binding sites of the tumor microenvironment.

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“…PD-1 aptamer/ Immunotherapy Lee et al, 2019 Proteins Constructing an autonomous DNA robot programmed to transport payloads and present them specifically in tumors.…”

Section: Resultsmentioning

confidence: 99%

“…In the presence of ATP, the scaffold containing ATP-responsive DNA motif and its complementary single-stranded DNA was changed from duplex to tertiary aptamer structure, resulting in the release of the embedded DOX from the duplex. Additionally, as an expected immune checkpoint inhibitor, PD-1 DNA aptamers were selected to construct hydrogels containing sgRNA sequences (Lee et al, 2019). Upon exposure to Cas9/sgRNA systems, the PD-1 DNA aptamer hydrogel lost its gel properties and released the PD-1 aptamer sequence to enhance antitumor immunotherapeutic effects (Figure 1Bi).…”

Section: Stimuli-responsive Drug Deliverymentioning

confidence: 99%

Endogenous Stimuli-Responsive DNA Nanostructures Toward Cancer Theranostics

Min

et al. 2020

Front. Nanotechnol.

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Nanostructures specifically responsive to endogenous biomolecules hold great potential in accurate diagnosis and precision therapy of cancers. In the pool of nanostructures with responsiveness to unique triggers, nanomaterials derived from DNA self-assembly have drawn particular attention due to their intrinsic biocompatibility and structural programmability, enabling the selective bioimaging, and site-specific drug delivery in cancer cells and tumor tissues. In this mini review, we summarize the most recent advances in the development of endogenous stimuli-responsive DNA nanostructures featured with precise self-assembly, targeted delivery, and controlled drug release for cancer theranostics. This mini review briefly discusses the diverse dynamic DNA nanostructures aiming at bioimaging and biomedicine, including DNA self-assembling materials, DNA origami structures, DNA hydrogels, etc. We then elaborate the working principles of DNA nanostructures activated by biomarkers (e.g., miRNA, mRNA, and proteins) in tumor cells and microenvironments of tumor tissue (e.g., pH, ATP, and redox gradient). Subsequently, applications of the endogenous stimuli-responsive DNA nanostructures in biological imaging probes for detecting cancer hallmarks as well as intelligent carriers for drug release in vivo are discussed. In the end, we highlight the current challenges of DNA nanotechnology and the further development of this promising research direction.

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Cas9-edited immune checkpoint blockade PD-1 DNA polyaptamer hydrogel for cancer immunotherapy

Cited by 68 publications

References 36 publications

Circular Nucleic Acids: Discovery, Functions and Applications

Circular Nucleic Acids: Discovery, Functions and Applications

Emerging Biomimetic Materials for Studying Tumor and Immune Cell Behavior

Endogenous Stimuli-Responsive DNA Nanostructures Toward Cancer Theranostics

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