Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response

Wang, Xiao; Ding, Chengfeng; Yu, Wenxia; Wang, Ying; He, Siting; Yang, Bei; Xiong, Yi–Ming; Wei, Jia; Li, Jifang; Liang, Junquan; Lu, Zongyang; Zhu, Wei; Wu, Jing; Zhou, Zhi; Huang, Xingxu; Liu, Zhen; Yang, Li; Chen, Jia

doi:10.1016/j.celrep.2020.107723

Cited by 70 publications

(56 citation statements)

References 58 publications

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“…The results demonstrated that the CBE system could be used to reduce off-target mutations. Recent developments in the BE system in plant species have been well documented in several independent studies [ 79 , 80 , 81 ]. The BE system has contributed significantly to elite germplasm development; however, there are fast-moving developments in GETs, and researchers are moving toward more reliable and easy techniques.…”

Section: Application Of Gets In Agriculture To Ensure Food Securitymentioning

confidence: 99%

Evolution and Application of Genome Editing Techniques for Achieving Food and Nutritional Security

Fiaz

Ahmar

Saeed

et al. 2021

IJMS

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A world with zero hunger is possible only through a sustainable increase in food production and distribution and the elimination of poverty. Scientific, logistical, and humanitarian approaches must be employed simultaneously to ensure food security, starting with farmers and breeders and extending to policy makers and governments. The current agricultural production system is facing the challenge of sustainably increasing grain quality and yield and enhancing resistance to biotic and abiotic stress under the intensifying pressure of climate change. Under present circumstances, conventional breeding techniques are not sufficient. Innovation in plant breeding is critical in managing agricultural challenges and achieving sustainable crop production. Novel plant breeding techniques, involving a series of developments from genome editing techniques to speed breeding and the integration of omics technology, offer relevant, versatile, cost-effective, and less time-consuming ways of achieving precision in plant breeding. Opportunities to edit agriculturally significant genes now exist as a result of new genome editing techniques. These range from random (physical and chemical mutagens) to non-random meganucleases (MegaN), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein system 9 (CRISPR/Cas9), the CRISPR system from Prevotella and Francisella1 (Cpf1), base editing (BE), and prime editing (PE). Genome editing techniques that promote crop improvement through hybrid seed production, induced apomixis, and resistance to biotic and abiotic stress are prioritized when selecting for genetic gain in a restricted timeframe. The novel CRISPR-associated protein system 9 variants, namely BE and PE, can generate transgene-free plants with more frequency and are therefore being used for knocking out of genes of interest. We provide a comprehensive review of the evolution of genome editing technologies, especially the application of the third-generation genome editing technologies to achieve various plant breeding objectives within the regulatory regimes adopted by various countries. Future development and the optimization of forward and reverse genetics to achieve food security are evaluated.

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Section: Application Of Gets In Agriculture To Ensure Food Securitymentioning

confidence: 99%

Evolution and Application of Genome Editing Techniques for Achieving Food and Nutritional Security

Fiaz

Ahmar

Saeed

et al. 2021

IJMS

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“…To reduce indel formation, more UGIs were fused into or coexpressed with nCas9-CBEs, which enhanced editing efficiency as well [72,73]. Differently, nCas9 could be replaced by dCpf1/dCas12a in some recently developed CBEs [59,74,75], which were shown to induce efficient C-to-T editing with only a basal level of DNA damage response [75], due to the fusion of catalytic dead dCpf1/dCas12a in these CBEs.…”

Section: Adopting Naturally Existing Cytidine Deaminase Effector For mentioning

confidence: 99%

A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing

Yang

Chen

2020

Trends in Biochemical Sciences

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Highlights CRISPR/Cas with different modules for independent target binding and cleavage has evolved to achieve convenient and precise genome editing. The endonuclease effector in conventional CRISPR/Cas genome editing systems can be replaced by nucleobase deaminases and the resulting base editors (BEs) enable single base changes. By fusing CRISPR/Cas with reverse transcriptases, prime editors (PEs) represent a new way to accomplish genetic changes, including all types of base substitutions, small indels, and their combinations. Both BEs and PEs are of potential in correcting disease-associated mutations. Genome-wide and/or transcriptomewide off-target mutations are catalyzed by the nucleobase deaminase effector in BEs, which are independent of the fused gRNA/Cas moiety.

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“…A recent report described a new cytosine base editing system called BEACON (base editing induced by human APOBEC3A and Cas12a without DNA break) that uses nuclease dead Cas12a fused to an engineered human apolipoprotein B mRNA editing enzyme, catalytic polypeptide‐like 3A (APOBEC3A). Editing efficiency was slightly lower than optimized systems such as BE3 and BE4max but indel introduction as well as RNA off‐target editing was minimal with BEACON (Wang et al, 2020b).…”

Section: Base Editingmentioning

confidence: 95%

Genome editing for plant research and crop improvement

Zhan¹,

Zhu

et al. 2021

JIPB

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The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control.

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Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response

Abstract: Highlights d BEACON induces basal levels of DNA breaks and DNA damage response d BEACON induces a basal level of RNA off-target mutations d BEACON induces in vivo base editing with high product purity

Cited by 70 publications

References 58 publications

Evolution and Application of Genome Editing Techniques for Achieving Food and Nutritional Security

Evolution and Application of Genome Editing Techniques for Achieving Food and Nutritional Security

A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing

Genome editing for plant research and crop improvement

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