Caryometric study of spermatogenesis in the rat

Ferreira, Adelina; Lison, L; Valeri, V.

doi:10.1007/bf00337032

Cited by 15 publications

(6 citation statements)

References 19 publications

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“…Finally, the number of tubules classified into each score of maturity was multiplied by its respective score, with the resultant values summed and then divided by 100, resulting in a "mean score". In adult animals (90 days), a more detailed evaluation of spermatogenesis was made in seminiferous tubules, classifying them into stages of I-VI, VII-VIII, IX-XIII and XIV, according to the number of generations of spermatids, presence of mature spermatids localized on the lumen border or secondary spermatocyte [32]. …”

Section: Methodsmentioning

confidence: 99%

Short- and long-term reproductive effects of prenatal and lactational growth restriction caused by maternal diabetes in male rats

Amorim

Damasceno

Perobelli

et al. 2011

Reprod Biol Endocrinol

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BackgroundA suboptimal intrauterine environment may have a detrimental effect on gonadal development and thereby increases the risk for reproductive disorders and infertility in adult life. Here, we used uncontrolled maternal diabetes as a model to provoke pre- and perinatal growth restriction and evaluate the sexual development of rat male offspring.MethodsMaternal diabetes was induced in the dams through administration of a single i.v. dose of 40 mg/kg streptozotocin, 7 days before mating. Female rats presenting glycemic levels above 200 mg/dL after the induction were selected for the experiment. The male offspring was analyzed at different phases of sexual development, i.e., peripuberty, postpuberty and adulthood.ResultsBody weight and blood glucose levels of pups, on the third postnatal day, were lower in the offspring of diabetic dams compared to controls. Maternal diabetes also provoked delayed testicular descent and preputial separation. In the offspring of diabetic dams the weight of reproductive organs at 40, 60 and 90 days-old was lower, as well as sperm reserves and sperm transit time through the epididymis. However the plasma testosterone levels were not different among experimental groups.ConclusionsIt is difficult to isolate the effects directly from diabetes and those from IUGR. Although the exposure to hyperglycemic environment during prenatal life and lactation delayed the onset of puberty in male rats, the IUGR, in the studied model, did not influenced the structural organization of the male gonads of the offspring at any point during sexual development. However the decrease in sperm reserves in epididymal cauda and the acceleration in sperm transit time in this portion of epididymis may lead to an impairment of sperm quality and fertility potential in these animals. Additional studies are needed in attempt to investigate the fertility of animals with intrauterine growth restriction by maternal diabetes and possible multigenerational effects.

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Section: Methodsmentioning

confidence: 99%

Short- and long-term reproductive effects of prenatal and lactational growth restriction caused by maternal diabetes in male rats

Amorim

Damasceno

Perobelli

et al. 2011

Reprod Biol Endocrinol

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“…Each animal was killed by decapitation 22 days after streptozotocin injection, and the right testis, epididymis, vas deferens and half ventral prostate were removed and fixed in Alfac fixing solution (80% ethanol, formaldehyde and glacial acetic acid, 8.5 : 1.0 : 0.5, v/v) for 24 h. After histological processing, 7 μ m‐thick paraffin sections were stained with haematoxylin and eosin. One hundred cross sections of seminiferous tubules per animal were randomly analysed in order to evaluate the relative frequency of stages I–VI, VII–VIII, IX–XIII and XIV of the germinal epithelium cycle (Ferreira et al. , 1967).…”

Section: Methodsmentioning

confidence: 99%

Sexual behaviour, sperm quantity and quality after short‐term streptozotocin‐induced hyperglycaemia in rats

et al. 2006

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Studies of diabetes mellitus in the streptozotocin rat model suggest that sexual dysfunctions may result from diabetes-induced alterations of the neuroendocrine-reproductive tract axis. Our investigation was performed to better define the effects of short-term hyperglycaemia on rat epididymal sperm quantity, quality and transit time, using both natural mating and artificial in utero insemination protocols. Male rats were made diabetic with streptozotocin (sc, 40 mg/kg), whereas controls received vehicle. Sexual behaviour was tested after 15 days and sperm fertilizing ability was checked 22 days after the injection through natural mating and artificial in utero insemination. Other parameters such as daily sperm production, testosterone levels, as well as sperm morphology and motility were also investigated. Fifty per cent of the diabetic animals showed no copulatory behaviour during tests and the number of animals reaching ejaculation was smaller in the diabetic group when compared with the control group (33% vs. 83%). Diabetes resulted in decreased body and reproductive organ weights, as well as diminished sperm counts in the testis and epididymis, that were associated with diminution of plasmatic testosterone levels. After natural mating, there was a decrease in the fertility in the diabetic adult male rats (25.5%) compared with control animals (81.5%). However, distal cauda epididymal sperm from diabetic rats displayed normal fertilization ability (91.5%) using in utero insemination. There were no effects of hyperglycaemia on sperm transit time in the epididymis and on spermatogenesis. Our results indicate that diabetes mellitus produces reproductive dysfunction, but does not compromise sperm fertilizing ability in the cauda epididymis in this experimental model.

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“…In order to determine the developmental stage of the germinal epithelium, 100 cross sections of seminiferous tubules per animal aged 41-61 days were analysed at random and scored as follows on the basis of the most advanced spermatid present in the epithelium: steps 1 to 8 of spermatid differentiation (score 2); steps 9 to 14 (score 4); steps 15 to 18 (score 6); fully mature spermatids in step 19 observed in small (score 8)) moderate (score 10) or maximal (score 12) amounts. The rate of the spermatogenic process in mature rats (91 days of age) was estimated by the relative frequency of stages I-VI, VII-VIII, IX-XI11 and XIV of the germinal epithelium cycle, identified according to Ferreira et al (1967).…”

Section: Methodsmentioning

confidence: 99%

Effects of guanethidine-induced sympathectomy on the spermatogenic and steroidogenic testicular functions of prepubertal to mature rats

Carvalho

Guimarães²,

Kempinas

et al. 2009

Andrologia

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Selective chemical sympathectomy of the internal genital organs of prepubertal to mature male Wistar rats was performed by chronic treatment with low doses of guanethidine. Sympathetic denervation caused an increase in intratesticular progesterone levels in prepubertal and early pubertal rats in addition to a decrease in androstenedione and testosterone levels in prepubertal animals, thus indicating a decrease in the conversion of progesterone into androgen, probably by blocking the steroidogenic enzymatic pathway at the 17 a-hydroxylase/ 17,20 desmolase level. A lower degree of testicular maturation, probably related to reduced androgen activity, was observed in prepubertal and early pubertal sympathectomized rats. Concentration of spermatozoa, on the other hand, was increased in the enlarged cauda epididymidis of late pubertal and mature denervated animals. This result is discussed in terms of the impairment of epididymal mechanisms of seminal emission, fluid resorption and spermatozoa1 disposal.

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Caryometric study of spermatogenesis in the rat

Cited by 15 publications

References 19 publications

Short- and long-term reproductive effects of prenatal and lactational growth restriction caused by maternal diabetes in male rats

Short- and long-term reproductive effects of prenatal and lactational growth restriction caused by maternal diabetes in male rats

Sexual behaviour, sperm quantity and quality after short‐term streptozotocin‐induced hyperglycaemia in rats

Effects of guanethidine-induced sympathectomy on the spermatogenic and steroidogenic testicular functions of prepubertal to mature rats

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