Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite‐induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats

Abstract

Background: The present study investigated the protective effects of carvacrol (CAR) against testicular toxicity induced by sodium arsenite (SA).Methods and Results: Rats were given SA and/or CAR for 14 days. Semen analyzes showed that CAR increased sperm motility and decreased the percentage of anarmol and dead sperm. It was determined that the oxidative stress induced by SA decreased with the increase of Nrf-2 and HO-1 expressions, SOD, CAT, GPx and GSH levels, and MDA levels decreased after CAR treatment. It was observed that autophagy and in ammation triggered by SA in testicular tissue were alleviated by suppressing the expressions of LC3A, LC3B, MAPK-14, NF-kB, TNF-a, IL-1b, iNOS and COX-2 biomarkers in rats given CAR. CAR treatment protected against apoptosis by suppressing Bax and Caspase-3 expressions and triggering Bcl-2 expression in testicles.Conclusions: As a result, it was seen that CAR could provide protection against SA-induced damage in testicular tissues of rats.

Cooling of porcine semen in an extender supplemented with carvacrol

“…In the first, the addition of carvacrol increased levels of antioxidants and improved sperm quality parameters (Güvenç et al, 2018), whereas, in other study, the C was able to increase endogenous enzymatic and non‐enzymatic antioxidants in a group previously treated with cisplatin, a chemotherapy drug (Aksu et al, 2016). In addition, other studies also report the ability of this molecule to enhance the activity of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase (Cicalău et al, 2021; Gur et al, 2023).…”

“…Finally, is remarkable that not much information is found approximately the use of C in sperm preservation, beyond therapeutic trials (Frydrychová et al, 2012;Gur et al, 2023;Güvenç et al, 2018). This study can be considered as a pioneer in the evaluation of carvacrol as an antioxidant for boar semen and opens the possibilities to evaluate other concentrations of this and its effect on other aspects of sperm integrity and metabolism.…”

“…In addition, other studies also report the ability of this molecule to enhance the activity of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase (Cicalău et al, 2021;Gur et al, 2023).…”

Catalpol ameliorates inflammation and oxidative stress via regulating Sirt1 and activating Nrf2/HO‐1 signaling against acute kidney injury

“…Numerous studies have suggested that inflammation and oxidative stress play an important role in the pathophysiology of various diseases, such as lung, testis, liver, thyroid, and kidney toxicity and pulmonary fibrosis 34–44 . Moreover, emerging findings exhibited that Cat has obvious anti‐inflammatory, antioxidant, and antiapoptotic properties and its vital protective function in multiple organ damage 45,46 .…”

“…Hence, it is essential to reveal the pathogenesis of SAKI, thereby paving the way for the develop- pulmonary fibrosis. [34][35][36][37][38][39][40][41][42][43][44] Moreover, emerging findings exhibited that Cat has obvious anti-inflammatory, antioxidant, and antiapoptotic properties and its vital protective function in multiple organ damage. 45,46 For instance, Xia et al manifested that Cat protected the spinal cord from spinal cord injury in mice through mediating microRNA-142-mediated HMGB1/TLR4/NF-κB signaling pathway.…”