Carvacrol as potential quorum sensing inhibitor of Pseudomonas aeruginosa and biofilm production on stainless steel surfaces

Tapia-Rodríguez, Melvin R.; Hérnández-Mendoza, Adrian; González‐Aguilar, Gustavo A.; Martínez-Téllez, Miguel Ángel; Martins, Claudia Miranda; Ayala-Zavala, J. Fernando

doi:10.1016/j.foodcont.2016.12.014

Cited by 90 publications

(48 citation statements)

References 24 publications

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“…For inhibiting biofilm bacteria, MBICs were determined as the lowest dose of each compound inhibiting the bacterial adhesion on stainless steel coupons (1 × 1 × 0.1 cm, grade 304) during 24 h of incubation at 37 • C [31]. Different concentrations of natural compounds (0-20 mg/mL) were added into test tubes with 10 mL of MH broth containing stainless steel coupons.…”

Section: Susceptibility Of Planktonic and Biofilm E Coli O157:h7 Celmentioning

confidence: 99%

“…Afterward, the coupons were placed in 5 mL of sterile peptone water and subjected to an ultrasonic bath (40 kHz) for 5 min to release the strongly adhered cells and were counted by plating on MH agar after 24 h of incubation at 37 • C (log CFU/cm 2 ). Both inhibitory concentrations were obtained by triplicate from three independent experiments, and the obtained results were expressed as mg/mL [31].…”

Section: Susceptibility Of Planktonic and Biofilm E Coli O157:h7 Celmentioning

confidence: 99%

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Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against Escherichia coli O157:H7

et al. 2020

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The resistance of Escherichia coli O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of Cymbopogon citratus (EO), have proven antibacterial activity against planktonic E. coli; however, no information was found about their efficacy and mode of action against E. coli biofilms. Therefore, the inhibitory effect of C. citratus EO, citral, and geraniol on glucans production and glucosyltransferase activity as anti-biofilm mechanism against E. coli was evaluated. EO, citral, and geraniol inhibited the planktonic growth of E. coli (minimal inhibitory concentration or MIC= 2.2, 1.0, and 3.0 mg/mL, respectively) and the bacterial adhesion (2.0, 2.0, and 4.0 mg/mL, respectively) on stainless steel. All compounds decreased the glucans production; citral and geraniol acted as uncompetitive inhibitors of glucosyltransferase activity (The half maximal inhibitory concentrations or IC50 were 8.5 and 6.5 µM, respectively). The evidence collected by docking analysis indicated that both terpenes could interact with the helix finger of the glucosyltransferase responsible for the polymer production. In conclusion, C. citratus EO, citral, and geraniol inhibited glucosyltransferase activity, glucans production, and the consequent biofilm formation of E. coli O157:H7.

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Section: Susceptibility Of Planktonic and Biofilm E Coli O157:h7 Celmentioning

confidence: 99%

Section: Susceptibility Of Planktonic and Biofilm E Coli O157:h7 Celmentioning

confidence: 99%

Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against Escherichia coli O157:H7

et al. 2020

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“…[25][26][27][28][29][30][31][32][33][34][35][36] Carvacrol (essential oil) was reported to inhibit biolm formation in S. aureus and P. aeruginosa and pyocyanin production in P. aeruginosa signicantly. 37 Baicalein (avonoid from the roots of Scutellaria baicalensis) was demonstrated to inhibit several virulence factors and biolm formation. 38 Another interesting study revealed that [6]-gingerol, a pungent active from fresh ginger which is structurally analogous to 3OC12-HSL, reduced biolm formation, virulence factors such as pyocyanin, exoprotease and rhamnolipid and mice mortality.…”

Section: Introductionmentioning

confidence: 99%

Potassium 2-methoxy-4-vinylphenolate: a novel hit exhibiting quorum-sensing inhibition inPseudomonas aeruginosa viaLasIR/RhlIR circuitry

et al. 2019

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“…This component can also disrupt bacterial lipids, RNA synthesis, ATPase activity and efflux pumps (Tapia‐Rodriguez et al . ).…”

Section: Resultsmentioning

confidence: 97%

Antibacterial and anti-PmrA activity of plant essential oils against fluoroquinolone-resistantStreptococcus pneumoniaeclinical isolates

Ghafari

Sharifi

Ahmadi

et al. 2018

Lett Appl Microbiol

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Streptococcus pneumoniae (pneumococcus) is recognized as one of the major cause of infections in communities and hospitals. In this study, anti-pneumococcal and anti-efflux pump activity of two medicinal plants (Thymus daenensis and Origanum vulgare) essential oils were evaluated. Checkerboard assay test was performed for investigation of the effects of selected EOs on ciprofloxacin (CIP) and ethidium bromide (EtBr) uptake in pmrA-overexpressed fluoroquinolone-resistant pneumococcus. Using quantitative real-time RT-PCR the PmrA efflux pump gene (pmrA) expression was evaluated following treatment with selected EOs. Gas chromatography-mass spectrometry analysis was performed for identifying the major components of the tested EOs. The minimum inhibitory concentration (MIC) values for pneumococcus isolates were 0·625-2·5 μl ml for T. daenensis and 1·25-5 μl ml for O. vulgare EOs. We confirmed that in all strains T. daenensis and O. vulgare have a total or partial synergistic effects with CIP and EtBr (FICI from 0·14 to 0·75). In other hand MIC/2 concentration of T. daenensis and O. vulgare EOs caused a significant downregulation of pmrA gene (P < 0·05) in seven of eight strains. This study showed that T. daenensis and O. vulgare EOs have strong antimicrobial and anti-efflux pump activity against clinical isolates of S. pneumoniae and might be useful in controlling pneumococcal infections. SIGNIFICANCE AND IMPACT OF THE STUDY: This study introduced Thymus daenensis and Origanum vulgare essential oil as new antibacterial and anti-efflux pump agents against fluoroquinolone-resistant Streptococcus pneumoniae clinical isolates. These findings indicate that combination of these two essential oils with fluoroquinolone antibiotics may provide alternative methods to overcome the fluoroquinolone-resistant S. pneumoniae.

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Carvacrol as potential quorum sensing inhibitor of Pseudomonas aeruginosa and biofilm production on stainless steel surfaces

Cited by 90 publications

References 24 publications

Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against Escherichia coli O157:H7

Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against Escherichia coli O157:H7

Potassium 2-methoxy-4-vinylphenolate: a novel hit exhibiting quorum-sensing inhibition inPseudomonas aeruginosa viaLasIR/RhlIR circuitry

Antibacterial and anti-PmrA activity of plant essential oils against fluoroquinolone-resistantStreptococcus pneumoniaeclinical isolates

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