Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester

Aigner, J.; Tegeler, J.; Hutzler, Peter; Campoccia, Davide; Pavesio, Alessandra; Hammer, C.; Kastenbauer, E.; Naumann, Andreas

doi:10.1002/(sici)1097-4636(199811)42:2<172::aid-jbm2>3.3.co;2-w

Cited by 82 publications

(75 citation statements)

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“…The individual effect of SM on construct properties was lower than that of SS, possibly because all of the SMs studied were biocompatible and supportive of chondrogenesis (e.g., 19,21,26,27). Although chondrocytes express specific, high-affinity hyaluronan receptors (56), otherwise comparable scaffolds made of Hyaff-11 ® or PGA NWM yielded constructs with similar properties.…”

Section: Discussionmentioning

confidence: 95%

“…Biomaterials used in previous cartilage engineering studies varied widely with respect to scaffold material, SM (e.g., synthetic, semi-synthetic, and naturally occurring polymers), scaffold structure, SS (e.g., hydrogels, fibrous meshes, and sponges), and with respect to mechanical properties and degradation rate. Examples of these biomaterials include gels of fibrin (9) or collagen (10,11) and meshes or sponges of collagen (12), polyglycolic acid (e.g., 8,13,[14][15][16][17], polylactic acid (e.g., 13,18) or cross-linked and/or derivatized hyaluronic acid (19)(20)(21). Identifying how a specific scaffold property affects chondrocyte attachment, proliferation, and/or differentiation has proven problematic, in part due to technical difficulties involved in fabricating two scaffolds that differ with respect to only one property.…”

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confidence: 99%

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Bioreactors mediate the effectiveness of tissue engineering scaffolds

et al. 2002

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We hypothesized that the mechanically active environment present in rotating bioreactors mediates the effectiveness of three-dimensional (3D) scaffolds for cartilage tissue engineering. Cartilaginous constructs were engineered by using bovine calf chondrocytes in conjunction with two scaffold materials (SM) (benzylated hyaluronan and polyglycolic acid); three scaffold structures (SS) (sponge, non-woven mesh, and composite woven/non-woven mesh); and two culture systems (CS) (a bioreactor system and petri dishes). Construct size, composition [cells, glycosaminoglycans (GAG), total collagen, and type-specific collagen mRNA expression and protein levels], and mechanical function (compressive modulus) were assessed, and individual and interactive effects of model system parameters (SM, SS, CS, SM*CS and SS*CS) were demonstrated. The CS affected cell seeding (higher yields of more spatially uniform cells were obtained in bioreactor-grown than dish-grown 3-day constructs) and subsequently affected chondrogenesis (higher cell numbers, wet weights, wet weight GAG fractions, and collagen type II levels were obtained in bioreactor-grown than dish-grown 1-month constructs). In bioreactors, mesh-based scaffolds yielded 1-month constructs with lower type I collagen levels and four-fold higher compressive moduli than corresponding sponge-based scaffolds. The data imply that interactions between bioreactors and 3D tissue engineering scaffolds can be utilized to improve the structure, function, and molecular properties of in vitro-generated cartilage.

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Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

Bioreactors mediate the effectiveness of tissue engineering scaffolds

et al. 2002

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“…5 They encompass either natural polymer scaffolds, that is, collagen, hyaluronic acid, alginate, or synthetic types made from poly(L-lactic acid) (PLLA), poly(glycolic acid) (PGA), and their copolymers (PLGA). [6][7][8][9][10][11] Compared to the natural scaffolds, synthetic scaffolds permit the use of a variety of techniques to fabricate porous scaffolds. They include solvent casting and particulate leaching (SC/PL), fabrication of a nonwoven sheet, phase separation, gas foaming, emulsion freeze drying, and 3D printing.…”

Section: Introductionmentioning

confidence: 99%

Beneficial effect of hydrophilized porous polymer scaffolds in tissue‐engineered cartilage formation

Park

Son

et al. 2007

J Biomed Mater Res

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Three dimensional (3D) porous poly(L-lactic acid) (PLLA) scaffolds were fabricated using a modified gas foaming method whose effervescent porogens were a mixture of sodium bicarbonate and citric acid. To improve chondrocyte adhesion, the scaffolds were then hydrophilized through oxygen plasma treatment and in situ graft polymerization of acrylic acid (AA). When the physical properties of AA-grafted scaffolds were examined, the porosity and pore size were 87 approximately 93% and 100 approximately 300 microm, respectively. The pore sizes were highly dependent on the varying ratios (w/w) between porogen and polymer solution. Influenced by their pore sizes, the compressive moduli of scaffolds significantly decreased with increasing pore size. The altered surface characteristics were clearly reflected in the reduced water contact angles that meant a significant hydrophilization with the modified polymer surface. Electron spectroscopy for chemical analysis (ESCA) and time-of-flight secondary ion mass spectrometer (ToF-SIMS) also confirmed the altered surface chemistry. When chondrocytes were seeded onto the AA-grafted PLLA scaffolds, cell adhesion and proliferation were substantially improved as compared to the unmodified scaffolds. The benefit of the modified scaffolds was clear in the gene expressions of collagen type II that was significantly upregulated after 4-week culture. Safranin-O staining also identified greater glycosaminoglycan (GAG) deposition in the modified scaffold. The AA-grafted porous polymer scaffolds were effective for cell adhesion and differentiation, making them a suitable platform for tissue-engineered cartilage.

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“…HA, similarly to collagen, can impart intrinsic signals within the scaffold that can enhance cell proliferation and tissue development. 60 Therefore, in recent years, HA-based materials were used for tissue engineering of skin, 61 cartilage, 62 and bone. 63 More recently, Remuzzi and colleagues have used esterified HA material as a scaffold for the production of vascular constructs.…”

Section: Hyaluronic Acid-based Scaffoldsmentioning

confidence: 99%

“Designer” scaffolds for tissue engineering and regeneration

Tsur-Gang

Cohen

2005

Israel Journal of Chemistry

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Tissue engineering entails the in vitro or in vivo generation of replacement tissues from cells with the aid of supporting scaffolds and stimulating biomolecules, in order to provide biological substitutes for restoration and maintenance of human tissue functions. In this review, we summarize the main classes of degradable polymeric scaffolds, natural and synthetic ones, and the evolution made in this field from adaptation of materials in clinical use to the fabrication of “designer” scaffolds.

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Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester

Cited by 82 publications

References 0 publications

Bioreactors mediate the effectiveness of tissue engineering scaffolds

Bioreactors mediate the effectiveness of tissue engineering scaffolds

Beneficial effect of hydrophilized porous polymer scaffolds in tissue‐engineered cartilage formation

“Designer” scaffolds for tissue engineering and regeneration

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