Cartilage tissue engineering using funnel-like collagen sponges prepared with embossing ice particulate templates

Lu, Hongxu; Ko, Young-Gwang; Naoki, Katsuhiko; Chen, Guoping

doi:10.1016/j.biomaterials.2010.04.019

Cited by 83 publications

(63 citation statements)

References 29 publications

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“…Embossing ice particulates were used as templates to prepare funnel-like collagen sponges using the previously reported method [10,12,13]. First, micron-sized hemispheric water droplets were formed by application of moisture to a perfluoroalkoxy (PFA) film-wrapped copper plate in a plastic chamber using an ultrasonic humidifier.…”

Section: Fabrication Of Funnel-like Hybrid Scaffoldmentioning

confidence: 99%

“…The ideal scaffold also should have an open and interconnected porous architecture, which helps cells easily penetrate into the inner part of the scaffold and results in homogenous cell distribution and tissue formation. We have developed a method using embossing ice particulate templates to prepare funnel-like porous scaffolds that have open surface pores and interconnected pore structures [10,11]. The funnel-like scaffolds facilitate cell penetration into the inner parts of the scaffolds and spatially homogeneous cell distribution.…”

Section: Introductionmentioning

confidence: 99%

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PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Naoki

et al. 2012

Science and Technology of Advanced Materials

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In skin tissue engineering, a three-dimensional porous scaffold is necessary to support cell adhesion and proliferation and to guide cells moving into the repair area in the wound healing process. Structurally, the porous scaffold should have an open and interconnected porous architecture to facilitate homogenous cell distribution. Moreover, the scaffolds should be mechanically strong to protect deformation during the formation of new skin. In this study, the hybrid scaffolds were prepared by forming funnel-like collagen or gelatin sponge on a woven poly(l-lactic acid) (PLLA) mesh. The hybrid scaffolds combined the advantages of both collagen or gelatin (good cell-interactions) and PLLA mesh (high mechanical strength). The hybrid scaffolds were used to culture dermal fibroblasts for dermal tissue engineering. The funnel-like porous structure promoted homogeneous cell distribution and extracellular matrix production. The PLLA mesh reinforced the scaffold to avoid deformation. Subcutaneous implantation showed that the PLLA-collagen and PLLA-gelatin scaffolds promoted the regeneration of dermal tissue and epidermis and reduced contraction during the formation of new tissue. These results indicate that funnel-like hybrid scaffolds can be used for skin tissue regeneration.

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Section: Fabrication Of Funnel-like Hybrid Scaffoldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Naoki

et al. 2012

Science and Technology of Advanced Materials

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“…For example, porous alginate showed enhanced cell proliferation and increased permeability by nearly three orders of magnitude when compared to nonporous conditions [38]. Porous collagen prepared with ice particulates improved cell distribution and chondrogenesis [39]. Moreover, the mechanical and transport properties of porous hydrogels are mainly dictated by pore size and hydrogel density [40]; In particular, pore size is also mandatory for regulating cell behavior, such as neovascularization [41].…”

Section: Polymer Based Macroporous Scaffolds For Osteochondral Tissuementioning

confidence: 99%

Hydrogel-Based Platforms for the Regeneration of Osteochondral Tissue and Intervertebral Disc

et al. 2012

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Hydrogels currently represent a powerful solution to promote the regeneration of soft and hard tissues. Primarily, they assure efficient bio-molecular interactions with cells, also regulating their basic functions, guiding the spatially and temporally complex multi-cellular processes of tissue formation, and ultimately facilitating the restoration of structure and function of damaged or dysfunctional tissues. In order to overcome basic drawbacks of traditional synthesized hydrogels, many recent strategies have been implemented to realize multi-component hydrogels based on natural and/or synthetic materials with tailored chemistries and different degradation kinetics. Here, a critical review of main strategies has been proposed based on the use of hydrogels-based devices for the regeneration of complex tissues, i.e., osteo-chondral tissues and intervertebral disc.

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“…Towards the design and development of ideal scaffold for optimal regenerative performance, a variety of scaffold fabrication techniques such as solvent casting/salt leaching, phase separation, and freeze-drying have been employed to generate scaffolds with distinct properties [4][5][6]. Although all these techniques have demonstrated their potential for bone tissue engineering application, a microsphere-based approach for tissue engineering initially developed by Laurencin et al appears to be extraordinarily attractive [7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

Lv¹,

Deng²,

Nair³

et al. 2014

Bioceram Dev Appl

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Polymeric sintered microsphere scaffolds have shown their tremendous potential in bone tissue engineering applications due to their highly porous and interconnected three dimensional structure and excellent mechanical properties. While these scaffolds are able to support basic cellular activity after seeding cells on them, the bioactivity of these scaffolds in terms of enhancing the biological performance of stem cells during bone regeneration is still under satisfactory. We hypothesized that incorporation of bioactive addictive such as hydroxyapatite into these scaffolds could improve their bioactivity without sacrificing the bulk properties of the scaffolds. We have successfully incorporated nano-hydroxyapatite (n-HA) into poly (lactic acid-glycolic acid) (PLAGA) microsphere based scaffolds in our previous studies. Herein, we aimed to evaluate the bioactivity of PLAGA/n-HA composite scaffolds, with a focus on studying the mineralization of the scaffolds in vitro. The capability of inducing apatite formation in vitro was largely enhanced in the composite scaffolds compared to plain PLAGA scaffolds. More importantly, PLAGA/n-HA composite scaffolds have been shown to improve rabbit mesenchymal stem cells (RMSCs) proliferation, differentiation, and mineralization as compared to control PLAGA scaffolds. Taken together, introduction of n-HA appears to be an efficient approach to improve the bioactivity of PLAGA scaffolds for bone tissue engineering.

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Cartilage tissue engineering using funnel-like collagen sponges prepared with embossing ice particulate templates

Cited by 83 publications

References 29 publications

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Hydrogel-Based Platforms for the Regeneration of Osteochondral Tissue and Intervertebral Disc

Evaluation of PLAGA/n-HA Composite Scaffold Bioactivity in vitro

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