Cartilage Tissue Engineering

Chang, Chih-Hung; Lin, Feng‐Huei; Kuo, Tzong-Fu; Liu, Hwa‐Chang

doi:10.4015/s101623720500010x

Cited by 26 publications

(34 citation statements)

References 75 publications

(74 reference statements)

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“…112 The scaffold should (1) be biodegradable in a controlled way without cytotoxic, tumorigenetic, nephrotoxic, or other undesirable effects; (2) have a porosity that allows diffusion of nutrients and waste products; (3) support cell viability, proliferation, differentiation, and ECM production; (4) be able to fix to and integrate with the tissue at the defect site; and (5) give mechanical support to the engineered tissue. 104,106 Ideally, as the ECM develops, the scaffold dissolves and disappears. 107 A comparison of chondrocyte cells cultured in rotating bioreactors in Space (Mir Space Station) and on Earth was reported by Freed et al 113 Freed and his team performed experiments with rotating cultures of bovine chondrocytes in polyglycolic acid scaffolds and concluded that after 7 months of cultivating the culture on Earth (static 1 g control) it produced cartilage tissues closer to the natural form than that in Space.…”

Section: Cartilagementioning

confidence: 99%

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Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Grimm

Wehland

Pietsch

et al. 2014

Tissue Engineering Part B: Reviews

121

137

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Tissue engineering in simulated (s-) and real microgravity (r-mg) is currently a topic in Space medicine contributing to biomedical sciences and their applications on Earth. The principal aim of this review is to highlight the advances and accomplishments in the field of tissue engineering that could be achieved by culturing cells in Space or by devices created to simulate microgravity on Earth. Understanding the biology of three-dimensional (3D) multicellular structures is very important for a more complete appreciation of in vivo tissue function and advancing in vitro tissue engineering efforts. Various cells exposed to r-mg in Space or to s-mg created by a random positioning machine, a 2D-clinostat, or a rotating wall vessel bioreactor grew in the form of 3D tissues. Hence, these methods represent a new strategy for tissue engineering of a variety of tissues, such as regenerated cartilage, artificial vessel constructs, and other organ tissues as well as multicellular cancer spheroids. These aggregates are used to study molecular mechanisms involved in angiogenesis, cancer development, and biology and for pharmacological testing of, for example, chemotherapeutic drugs or inhibitors of neoangiogenesis. Moreover, they are useful for studying multicellular responses in toxicology and radiation biology, or for performing coculture experiments. The future will show whether these tissue-engineered constructs can be used for medical transplantations. Unveiling the mechanisms of microgravity-dependent molecular and cellular changes is an up-to-date requirement for improving Space medicine and developing new treatment strategies that can be translated to in vivo models while reducing the use of laboratory animals.

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Section: Cartilagementioning

confidence: 99%

“…106,107 Chondrocytes are the most obvious cell source for the tissue engineering of cartilage. 108 However, chondrocyte expansion in a monolayer causes cell dedifferentiation, characterized by decreased proteoglycan synthesis and type II collagen expression, and increased type I collagen expression.…”

Section: Cartilagementioning

confidence: 99%

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Grimm

Wehland

Pietsch

et al. 2014

Tissue Engineering Part B: Reviews

121

137

View full text Add to dashboard Cite

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“…Dissected stifle joints of 4-week-old cadaver piglets were prepared at the Vaccine and Infection Disease Organization (VIDO). For each sample, a full-thickness articular defect (4.5 · 4.5 · 2-2.5 mm depth) was created in the lateral femoral condyle cartilage of the knee, and a scaffold as prepared earlier was then implanted into the created defect area and covered with a piece of periosteum layer, 14 which was concurrently harvested from the proximal femur ( Fig. 2), to mimic the actual surgical procedure used in animal and clinical studies.…”

Section: Sample Preparationmentioning

confidence: 99%

Computed Tomography Diffraction-Enhanced Imaging forIn SituVisualization of Tissue Scaffolds Implanted in Cartilage

IzadifarZohreh

Dean²,

Chen³

2014

Tissue Engineering Part C: Methods

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Long-term in vivo studies on animal models and advances from animal to human studies should rely on noninvasive monitoring methods. Synchrotron radiation (SR)-diffraction enhanced imaging (DEI) has shown great promise as a noninvasive method for visualizing native and/or engineered tissues and bio-microstructures with appreciable details in situ. The objective of this study was to investigate SR-DEI for in situ visualization and characterization of tissue-engineered scaffolds implanted in cartilage. A piglet stifle joint implanted with an engineered scaffold made from poly-ɛ-caprolactone was imaged using SR computed tomography (CT)-DEI at an X-ray energy of 40 keV. For comparison, in situ visualization was also conducted with commonly used SR CT-phase contrast imaging and clinical magnetic resonance imaging techniques. The reconstructed CT-DE images show the implanted scaffold with the structural properties much clearer than those in the CT-PC and MR images. Furthermore, CT-DEI was able to visualize microstructures within the cartilage as well as different soft tissues surrounding the joint. These microstructural details were not recognizable using other imaging techniques. Taken together, the results of this study suggest that CT-DEI can be used for noninvasive visualization and characterization of scaffolds in cartilage, representing an advance in tissue engineering to track the success of tissue scaffolds for cartilage repair.

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“…As propriedades mecânicas e físico-químicas dos arcabouços utilizados na Engenharia de Tecidos são características importantes, que influenciam a sua aplicação final. Estes arcabouços não só apresentam uma função mecânica, como também apoiam a fixação, migração, proliferação e diferenciação celular para expressão de fenótipos desejáveis [16,19] . As propriedades mecânicas dos arcabouços como a resistência à tração, flexão e compressão, ductilidade (propriedade de suportar a deformação sem romper ou fraturar) e módulo de Young (razão entre tensão e deformação no regime elástico) são determinadas tanto pelas propriedades do material quanto pela estrutura do mesmo (macro, micro e nanoestrutura).…”

Section: Arcabouços Para Cultura De Célulasunclassified

“…Durante a formação, deposição e organização da nova matriz, o ideal é que o arcabouço seja degradado e metabolizado, deixando que o órgão vital ou tecido seja reestabelecido, mantenha ou melhore sua função tecidual [25] . É importante sincronizar o tempo de degradação/absorção do biomaterial com o tempo de regeneração do tecido, possibilitando a transferência gradativa das funções do biomaterial para o tecido recém-formado, não devendo, contudo, originar produtos de degradação que possam interferir com o tecido em crescimento ou originar um processo inflamatório [15,19] . Na escolha do biomaterial, deve-se ter ainda em consideração, que há matrizes cujas características químicas definem a sua suscetibilidade à degradação aquosa ou enzimática [9,15] .…”

Section: Arcabouços Para Cultura De Célulasunclassified

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

Nascimento

Lombello

2016

Polímeros

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AesumoA Engenharia de Tecidos envolve o desenvolvimento de novos materiais ou dispositivos capazes de interações específicas com os tecidos biológicos, buscando a utilização de materiais biocompatíveis que devem servir como arcabouço para o crescimento de células in vitro, organizando e desenvolvendo o tecido que posteriormente será implantado no paciente. Uma variedade de arcabouços como hidrogéis poliméricos, sintéticos e naturais, têm sido investigados para a expansão de condrócitos in vitro, visando o reparo da cartilagem lesionada. Um hidrogel de interesse particular na regeneração de cartilagem é o ácido hialurónico (AH). Trata-se de um biopolímero atraente para a fabricação de arcabouços artificiais para Engenharia de Tecidos por ser biocompatível e biodegradável. A biocompatibilidade do AH deve-se ao fato de estar presente na matriz extracelular nativa, deste modo, cria-se um ambiente propício que facilita a adesão, proliferação e diferenciação celular, além da existência de sinalização celular específica, o que contribui para a regeneração do tecido. O uso de hidrogel composto de ácido hialurónico e quitosana (QUI) também tem sido investigado em aplicações de Engenharia de Tecidos de cartilagem, com resultados promissores. Baseando-se nestas informações, o objetivo este trabalho foi investigar as alternativas disponíveis para regeneração tecidual da cartilagem e conhecer mais detalhadamente as relações entre células e biomateriais. Palavras-chave: ácido hialurônico, biocompatibilidade, engenharia de tecidos, quitosana, cultura de células. AbstractTissue Engineering involves the development of new materials or devices capable of specific interactions with biological tissues, searching the use of biocompatible materials as scaffolds for cell growth in vitro, organizing and developing tissue that is subsequently implanted into the patient. A variety of scaffolds such as polymeric hydrogels, natural and synthetic, have been investigated for the expansion of chondrocytes in vitro in order to repair the damaged cartilage. A hydrogel of particular interest in cartilage regeneration is hyaluronic acid (HA). HA are attractive biopolymers for manufacturing artificial scaffolds for Tissue Engineering, it is biocompatible and biodegradable. The biocompatibility of HA is due to the fact that it is present in native extracellular matrix, thus creates an environment, which facilitates the adhesion, proliferation and differentiation, in addition to the existence of specific cell signaling, which contributes to tissue regeneration. The use of hydrogel composed of hyaluronic acid and chitosan (CHI) has also been investigated for applications in Tissue Engineering of soft tissues, like cartilage, with promising results. Based on this information, this study aims to investigate the alternatives available for cartilage tissue regeneration and meet more detail the relationships between cells and biomaterials.

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Cartilage Tissue Engineering

Abstract: ABSTRACT

Cited by 26 publications

References 75 publications

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Computed Tomography Diffraction-Enhanced Imaging forIn SituVisualization of Tissue Scaffolds Implanted in Cartilage

Hidrogéis a base de ácido hialurônico e quitosana para engenharia de tecido cartilaginoso

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