Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse

The Role of Microrna-29 in Osteoarthritis

“…Whilst the initial plan was to use the acan-creERT2 mouse to facilitate cartilage-specific and inducible miR-29 knockout, subsequent communication with Prof. George Bou-Gharios (also see Kanakis et al, 2021) suggested this may not be the most effective system to use.…”

“…Subsequent to breeding of the acan-creERT2 mouse, communication from Prof. George Bou-Gharios indicated that not all chondrocytes in these mice were expressing the transgene leading to poor tissue-specific knockout (also discussed in Kanakis et al, 2021).…”

“…Cartilage-specific knockout of miR-29 may overcome the aforementioned complications associated with global knockout. Many cartilage-specific knockout mouse models exist and these have been used to study to the role of a wide range of genes in cartilage development, homeostasis and disease (Kanakis et al, 2021). In models utilising the Cre-loxP system, expression of Cre recombinase is under the control of a cartilage-specific promoter (e.g.,…”