Cartilage Repair

Clair, Benjamin L.; Johnson, Adam R.; Howard, Timothy D.

doi:10.1177/1938640009342272

Cited by 54 publications

(18 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current treatments for cartilage loss include autologous chondrocyte transplantation procedure, arthroscopic lavage and debridement, the subchondral bone microfracture technique, and osteochondral allograft [23]–[25]. These procedures may relieve the symptoms temporarily, but are often associated with problems such as donor site morbidity, loss of chondrocyte phenotype during in vitro expansion, fibrocartilage formation, and cartilage degeneration [26]–[28].…”

Section: Discussionmentioning

confidence: 99%

The Effect of 3D Nanofibrous Scaffolds on the Chondrogenesis of Induced Pluripotent Stem Cells and Their Application in Restoration of Cartilage Defects

Nie

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

The discovery of induced pluripotent stem cells (iPSCs) rendered the reprogramming of terminally differentiated cells to primary stem cells with pluripotency possible and provided potential for the regeneration and restoration of cartilage defect. Chondrogenic differentiation of iPSCs is crucial for their application in cartilage tissue engineering. In this study we investigated the effect of 3D nanofibrous scaffolds on the chondrogenesis of iPSCs and articular cartilage defect restoration. Super-hydrophilic and durable mechanic polycaprolactone (PCL)/gelatin scaffolds were fabricated using two separate electrospinning processes. The morphological structure and mechanical properties of the scaffolds were characterized. The chondrogenesis of the iPSCs in vitro and the restoration of the cartilage defect was investigated using scanning electron microscopy (SEM), the Cell Counting Kit-8 (CCK-8), histological observation, RT-qPCR, and western blot analysis. iPSCs on the scaffolds expressed higher levels of chondrogenic markers than the control group. In an animal model, cartilage defects implanted with the scaffold-cell complex exhibited an enhanced gross appearance and histological improvements, higher cartilage-specific gene expression and protein levels, as well as subchondral bone regeneration. Therefore, we showed scaffolds with a 3D nanofibrous structure enhanced the chondrogenesis of iPSCs and that iPSC-containing scaffolds improved the restoration of cartilage defects to a greater degree than did scaffolds alone in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

The Effect of 3D Nanofibrous Scaffolds on the Chondrogenesis of Induced Pluripotent Stem Cells and Their Application in Restoration of Cartilage Defects

Nie

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…[173] Other clinical procedures are also available, including arthroscopic lavage and debridement, microfracture techniques, and osteochondral transplantation. [174] While there have been some promising results, most cartilage repair techniques lead to fibrocartilage formation and cartilage degeneration after a temporary relief of symptoms. As such, the most effective procedure utilizes surgical replacement with an autograft.…”

Section: Engineering Stem Cells For Tissue Regenerationmentioning

confidence: 99%

Engineering Stem Cells for Biomedical Applications

Yin

Han

Lee

2015

Adv Healthcare Materials

View full text Add to dashboard Cite

Stem cells are characterized by a number of useful properties, including their ability to migrate, differentiate, and secrete a variety of therapeutic molecules such as immunomodulatory factors. As such, numerous pre-clinical and clinical studies have utilized stem cell-based therapies and demonstrated their tremendous potential for the treatment of various human diseases and disorders. Recently, efforts have focused on engineering stem cells in order to further enhance their innate abilities as well as to confer them with new functionalities, which can then be used in various biomedical applications. These engineered stem cells can take on a number of forms. For instance, engineered stem cells encompass the genetic modification of stem cells as well as the use of stem cells for gene delivery, nanoparticle loading and delivery, and even small molecule drug delivery. The present Review gives an in-depth account of the current status of engineered stem cells, including potential cell sources, the most common methods used to engineer stem cells, and the utilization of engineered stem cells in various biomedical applications, with a particular focus on tissue regeneration, the treatment of immunodeficiency diseases, and cancer.

show abstract

“…1 Since then, various techniques including debridement and lavage, marrow stimulation, autologous chondrocyte implantation (ACI), the osteochondral autograft transfer system (OATS), tissue engineering and other techniques have been explored to improve cartilage regeneration, some of which have found their way to the clinic. 2, 3 However, to date, no technique has reliably regenerated the biological composition and biomechanical properties of native cartilage, leaving unresolved pain and loss of joint function for millions of patients with defective cartilage from aging, injury, or disease.…”

Section: Introductionmentioning

confidence: 99%

“…Since the range of potential cell sources for cartilage regeneration is so extensive, the following criteria may help identify the best candidate cartilage regeneration cell sources: 1) easy to isolate and collect, 2) easy to expand in vitro to yield a large number of cells, 3) capable of expressing and synthesizing cartilage-specific molecules, 4) capable of producing neocartilage with comparable mechanical properties to native cartilage, 5) capable of integrating into the surrounding recipient site, and 6) immunocompatible. 2, 7, 8 With regard to these design goals, we have reviewed the cell sources used for cartilage lesion treatment and the trajectory of the field. We have compared potential cell sources, discussed their function in cartilage regeneration and assessed their success in in vitro models and clinical experiences.…”

Section: Introductionmentioning

confidence: 99%

Role of cartilage-forming cells in regenerative medicine for cartilage repair

Sun

Reagan²,

Kaplan

2010

ORR

View full text Add to dashboard Cite

Currently, cartilage repair remains a major challenge for researchers and physicians due to its limited healing capacity. Cartilage regeneration requires suitable cells; these must be easily obtained and expanded, able to produce hyaline matrix with proper mechanical properties, and demonstrate sustained integration with native tissue. At present, there is a wide variety of possible cell sources for cartilage regeneration; this review explores the diversity of sources for cartilage forming cells and the distinctive characteristics, advantages, limitations, and potential applications of each cell source. We place emphasis on cell sources used for in vitro and clinical studies.

show abstract

Cartilage Repair

Cited by 54 publications

References 90 publications

The Effect of 3D Nanofibrous Scaffolds on the Chondrogenesis of Induced Pluripotent Stem Cells and Their Application in Restoration of Cartilage Defects

The Effect of 3D Nanofibrous Scaffolds on the Chondrogenesis of Induced Pluripotent Stem Cells and Their Application in Restoration of Cartilage Defects

Engineering Stem Cells for Biomedical Applications

Role of cartilage-forming cells in regenerative medicine for cartilage repair

Contact Info

Product

Resources

About