Cartilage repair evolution in post-traumatic osteochondral lesions of the talus: From open field autologous chondrocyte to bone-marrow-derived cells transplantation

Giannini, Sandro; Buda, Roberto; Cavallo, Marco; Ruffilli, Alberto; Cenacchi, Annarita; Cavallo, Carola; Vannini, Francesca

doi:10.1016/j.injury.2010.09.028

Cited by 165 publications

(200 citation statements)

References 53 publications

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“…Ta ostat nia kwe stia mo że szcze gól nie do ty czyć cho rych z od dzie la ją cą mar twi ca chrzest no -kost ną. Peł ną oce nę pre zen to wa nej me to dy bę dzie mo żna uzy skać w ba da niach kli nicz nych po rów nu ją cych po szczegól ne me to dy, jak opu bli ko wa li to in ni [17][18][19]. Niewąt pli wie le cze nie ubyt ków chrzęst no -kost nych czy chrząst ki za po mo cą me zen chy mal nych ko mó rek ma cie rzy stych ma ogrom ną przy szłość ze wzglę du na pla stycz ność i ogrom ny po ten cjał [20].…”

Section: Discussionunclassified

“…This latter issue may be particularly relevant in the case of patients with osteochondritis dissecans. Full assessment of the method may be performed during a clinical study comparing individual techniques, as described by other authors [17][18][19]. The treatment of osteochondral or cartilage lesions with mesenchymal stem cells is a promising approach due to its plasticity and a substantial potential [20].…”

Section: Wnio Skimentioning

confidence: 99%

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Osteochondral lesions of the knee reconstructed with mesenchymal stem cells – results.

Skowroński¹,

Rutka²

2013

Ortop Traumatol Rehabil

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mó rek szpi ku. Material i metody. Ma te riał kli nicz ny sta no wi ło 46 pa cjen tów z ubyt ka mi chrzęst no -kost ny mi o ró żnej etiolo gii, po twier dzo ny mi w MRI, w dwóch gru pach: 21 cho rych za opa trzo nych z uży ciem kon cen tra tu ko mó rek szpiku i 25 cho rych le czo nych za po mo cą ko mó rek me zen chy mal nych uzy ska nych z krwi ob wo do wej. Oce nę przepro wa dzo no po 6 mie sią cach, 1 ro ku i 5 lat po re kon struk cji ubyt ku na pod sta wie skal: KO OS, Ly shol ma i VAS.Wy ni ki. Au to rzy uzy ska li istot ną po pra wę we wszyst kich ska lach ocen u 40 ope ro wa nych pa cjen tów (86%). U 6 cho rych od no to wa no wy nik zły. Wy ka za no istot ną sta ty stycz nie ró żni cę w gru pach po rów ny wanych na ko rzyść cho rych le czo nych ko mór ka mi ma cie rzy sty mi po zy ska ny mi z krwi. Oce na ob ra zów MRI, u cho rych z wy ni ka mi do bry mi i bar dzo do bry mi, wy ka za ła za do wa la ją cą od bu do wę po wierzch ni chrzęst nej oraz do brą in te gra cję re ge ne ra tu. Po 5-ciu la tach od no to wa no nie wiel ki spa dek śred niej ocen kli nicz nych w obu gru pach cho rych.Wnio ski. 1. Re kon struk cja z uży ciem zmo dy fi ko wa nej tech ni ki ka nap ko wej jest sku tecz ną me to dą le czenia głę bo kich ubyt ków chrzęst no -kost nych sta wu ko la no we go. 2. Nie co gor sze wy ni ki w gru pie le czo nej koncen tra tem ko mó rek szpi ku mo że mieć swą przy czy nę w licz bie po da nych ko mó rek ma cie rzy stych.Sło wa klu czo we: cho ro by chrząst ki, mar twi ca chrzęst no -kost na, ko mór ki ma cie rzy ste, chi rur gia re kon struk cyj na SUM MA RY Background. We pre sent the re sults of the tre at ment of oste ochon dral le sions with the mo di fied san dwich tech ni que with a col la gen mem bra ne. The aim of the stu dy was to as sess and com pa re cli ni cal out co mes fol lowing the re con struc tion of oste ochon dral le sions in two gro ups of pa tients tre ated with stem cells ob ta ined from blo od and with bo ne mar row con cen tra te.Material and methods. The stu dy gro up com pri sed 46 pa tients with MRI -con fir med oste ochon dral le sions of va rio us aetio lo gy. A gro up of 21 pa tients was tre ated with bo ne mar row con cen tra te and 25 pa tients we re treated with me sen chy mal cells ob ta ined from pe ri phe ral blo od. Pa tients we re as ses sed with the use of KO OS, Lysholm and VAS sca les at 6 mon ths, and at 1 and 5 years fol lo wing the re con struc tion.Re sults. We no ted a si gni fi cant im pro ve ment across all sca les in 40 pa tients (86%). A po or out co me was no ted in 6 pa tients. The re was a sta ti sti cal ly si gni fi cant su pe rio ri ty of the gro up tre ated with stem cells ob ta ined from blo od. The ana ly sis of MRI evi den ce in pa tients with go od and excel lent re sults sho wed sa tis fac to ry recon struc tion of the car ti la gi no us sur fa ce and go od re ge ne ra te in te gra tion. At 5 years, a sli ght de cre ase in me an cli ni cal as ses sment sco res was se en in both gro ups of pa tients.Conc lu sions. 1. The mo di fied san dwich...

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Section: Discussionunclassified

Section: Wnio Skimentioning

confidence: 99%

Osteochondral lesions of the knee reconstructed with mesenchymal stem cells – results.

Skowroński¹,

Rutka²

2013

Ortop Traumatol Rehabil

View full text Add to dashboard Cite

show abstract

“…However, this cell-therapy approach is limited by critical drawbacks, such as donor site morbidity and loss of the chondrocyte phenotype [41]. Therefore, in recent years hASCs have emerged as valuable tool for the regeneration of articular cartilage based on their ability to undergo differentiation into the chondrogenic lineage [42].…”

Section: Discussionmentioning

confidence: 99%

Chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor

Pirrone¹,

Gobbetti²,

Caprara³

et al. 2017

J Stem Cell Res Med

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In recent years, various clinical trials are exploring the efficacy of cell-based strategies to treat cartilage defects. Human adipose-derived stem/stromal cells (hASCs) have great potential for the regeneration of articular cartilage due to their ability to undergo differentiation into the chondrogenic lineage. In most cell-based therapies, the single dose requires 10 7 -10 9 cells, and then it is necessary to move from manual flask-based culture to bioreactor whose monitoring and control of biological variability is necessary. Furthermore, before clinical use, the success of hASC differentiation need to be thoroughly inspected. In this study, we sought to assess with complementary methods the chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor (HFBR). Human ASCs, after phenotypic characterization were subjected to chondrogenic differentiation, the expression of cartilage-specific genes was analysed by quantitative real-time polymerase chain reaction (qPCR); optical microscopy as well as transmission electron microscopy (TEM) were also performed. The two culture expansion systems do not affect hASC immunophenotype; microscopy analysis confirmed the successful of hASC differentiation process. Gene expression analysis after differentiation displayed an upregulation of cartilage-specific genes more noticeable in hASCs expanded in the HFBR, suggesting that hASCs expanded in the HFBR can be efficiently differentiated into chondroblasts. Our study demonstrates that flask and HFBR expansion methods do not affect the subsequent differentiation process; additionally, our results highlight the importance of analyzing differentiation by different techniques and support the importance of TEM analysis that is able to give additional critical information to molecular biology results.

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“…Several clinical studies have shown that clinical outcomes improved using this procedure [49][50][51][52][53]54•]. Giannini et al [53] evaluated patients who underwent BMDCT and demonstrated improved AOFAS scores from 63.7 to 82.2 in 49 patients at a mean follow-up of 48 months.…”

Section: Bone Marrow-derived Cell Transplantationmentioning

confidence: 99%

Osteochondral lesions of the talus in the athlete: up to date review

Shimozono

Yasui

Ross

et al. 2017

Curr Rev Musculoskelet Med

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Purpose of review Osteochondral lesions of the talus (OLT) are common injuries in athletes. The purpose of this study is to comprehensively review the clinical results and return to sport capacity in athletes following treatment for OLT. Recent findings Reparative procedures, such as bone marrow stimulation, and replacement procedures, such as autologous osteochondral transplantation, provide good clinical outcomes in short-and mid-term follow-up in the athlete. Recently, biological augmentation and scaffold-based therapies have been shown to improve clinical and radiological outcomes in OLT in both the general population and athletes. Summary Most studies are of a low level of evidence. Studies analyzing the return to sport capability in athletes are further lacking. High-level evidence and well-designed clinical trials are required to establish the most effective treatment protocol.

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Cartilage repair evolution in post-traumatic osteochondral lesions of the talus: From open field autologous chondrocyte to bone-marrow-derived cells transplantation

Cited by 165 publications

References 53 publications

Osteochondral lesions of the knee reconstructed with mesenchymal stem cells – results.

Osteochondral lesions of the knee reconstructed with mesenchymal stem cells – results.

Chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor

Osteochondral lesions of the talus in the athlete: up to date review

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