Cartilage regeneration using mesenchymal stem cells and a PLGA–gelatin/chondroitin/hyaluronate hybrid scaffold

Fan, Hongbin; Hu, Yunyu; Zhang, Chunli; Liu, Xusheng; Lü, Rong; Qin, Ling; Zhu, Rui

doi:10.1016/j.biomaterials.2006.04.013

Cited by 184 publications

(136 citation statements)

References 29 publications

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“…This seems to be contradictory to the fact that increased cell penetration was observed in scaffolds containing HA. The result also contrasted to previous observations suggesting a mitogenic effect of HA in scaffolds [29,30,45]. In our case, we suggest that the observed result may be based on the accumulation of cells on the surface of the control SF:HA (100:0) scaffolds that could compensate for the reduced population in the core.…”

Section: Scaffold Design: Composition and Microstructurecontrasting

confidence: 91%

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Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

et al. 2009

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Section: Scaffold Design: Composition and Microstructurecontrasting

confidence: 91%

“…The capacity of HA to enhance GAG deposition in tissue engineered constructs is in agreement with other work related to HA-based scaffolds [29,45] and underlines the current interest in HA-based biomaterials for tissue engineering applications [12,18,47].…”

Section: Scaffold Design: Composition and Microstructuresupporting

confidence: 88%

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

et al. 2009

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“…A previous study with OPF hydrogel encapsulating chondrocytes and gelatin microparticles showed a significant increase in GAG production as compared with OPF hydrogels alone [7]. Additionally, it has been reported that rabbit MSCs embedded in PLGA scaffolds modified with gelatin and chondroitin sulfate also showed the increase in GAG production as compared to PLGA alone [21]. However, it appears that MSCs encapsulated in OPF hydrogel composites produced less GAG than when cultured on scaffolds with pore sizes of 500 μm [21].…”

Section: Discussionmentioning

confidence: 89%

Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering

et al. 2007

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We investigated the development of an injectable, biodegradable hydrogel composite of oligo(poly (ethylene glycol) fumarate) (OPF) with encapsulated rabbit marrow mesenchymal stem cells (MSCs) and gelatin microparticles (MPs) loaded with transforming growth factor-β1 (TGF-β1) for cartilage tissue engineering applications. Rabbit MSCs and TGF-β1-loaded MPs were mixed with OPF, a poly (ethylene glycol)-diacrylate crosslinker and the radical initiators ammonium persulfate and N,N,N',N'-tetramethylethylenediamine, and then crosslinked at 37°C for 8 min to form hydrogel composites. Three studies were conducted over 14 days in order to examine the effects of: 1) the composite formulation, 2) the MSC seeding density, and 3) the TGF-β1 concentration on the chondrogenic differentiation of encapsulated rabbit MSCs. Bioassay results showed no significant difference in DNA amount between groups, however, groups with MPs had a significant increase in glycosaminoglycan content per DNA starting at day 7 as compared to controls at day 0. Chondrocytespecific gene expression of type II collagen and aggrecan were only evident in groups containing TGF-β1-loaded MPs and varied with TGF-β1 concentration in a dose dependent manner. Specifically, type II collagen gene expression exhibited a 161 ± 49 fold increase and aggrecan gene expression a 221 ± 151 fold increase after 14 days with the highest dose of TGF-β1 (16 ng/ml). These results indicate that encapsulated rabbit MSCs remained viable over the culture period and differentiated into chondrocyte-like cells, thus suggesting the potential of OPF composite hydrogels as part of a novel strategy for localized delivery of stem cells and bioactive molecules.

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“…One of the promising treatment approaches tested in preclinical and clinical studies is the transplantation of stem cells into the damaged spinal cord. 3,4 Mesenchymal stem cells (MSC) are under intensive study as a potential therapeutic tool, particularly for the treatment of bone, 5 cartilage, 6 cardiac 7 and neural diseases, including stroke, 8 amyotrophic lateral sclerosis, 9 and SCI. 10,11 Numerous studies have demonstrated their benefit in promoting anatomic and functional recovery after transplantation in animal models of SCI.…”

Section: Introductionmentioning

confidence: 99%

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Vaněček¹,

Zablotskii²,

Forostyak³

et al. 2012

IJN

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Abstract:The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number of transplanted cells that reach the lesioned tissue is one of the critical parameters. In this study, intrathecally transplanted cells labeled with superparamagnetic iron oxide nanoparticles were guided by a magnetic field and successfully targeted near the lesion site in the rat spinal cord. Magnetic resonance imaging and histological analysis revealed significant differences in cell numbers and cell distribution near the lesion site under the magnet in comparison to control groups. The cell distribution correlated well with the calculated distribution of magnetic forces exerted on the transplanted cells in the subarachnoid space and lesion site. The kinetics of the cells' accumulation near the lesion site is described within the framework of a mathematical model that reveals those parameters critical for cell targeting and suggests ways to enhance the efficiency of magnetic cell delivery. In particular, we show that the targeting efficiency can be increased by using magnets that produce spatially modulated stray fields. Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury.

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Cartilage regeneration using mesenchymal stem cells and a PLGA–gelatin/chondroitin/hyaluronate hybrid scaffold

Cited by 184 publications

References 29 publications

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

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