Cartilage regeneration using decellularized cartilage matrix: Long-term comparison of subcutaneous and intranasal placement in a rabbit model

Bomhard, Achim von; Elsaesser, Alexander F.; Riepl, Rudolf L.; Pippich, Katharina; Faust, Joseph; Schwarz, Silke; Koerber, Ludwig; Breiter, Roman; Rotter, Nicole

doi:10.1016/j.jcms.2019.01.010

Cited by 16 publications

(26 citation statements)

References 33 publications

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“…To date, several groups have shown promising results in developing DC‐ECM‐based scaffolds for regulation of both in vitro and in vivo chondrogenic differentiation (Benders, Terpstra, Levato, & Malda, 2019; Townsend et al, 2018; von Bomhard et al, 2019). Our previous in vitro studies have similarly shown that successful chondrogenic differentiation and matrix formation happens on the presence of DC‐ECM components within a tissue‐engineered matrix or cell culture media (Masaeli, Karamali, Loghmani, Eslaminejad, & Nasr‐Esfahani, 2017; Zahiri, Masaeli, Poorazizi, & Nasr‐Esfahani, 2018).…”

Section: Discussionmentioning

confidence: 99%

An in vivo evaluation of induced chondrogenesis by decellularized extracellular matrix particles

Masaeli

Nasr‐Esfahani

2020

J Biomedical Materials Res

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Bioengineered scaffolds composed of synthetic materials and extracellular matrix (ECM) components can offer a tissue‐specific microenvironment capable of regulating cells to regenerate the structure and function of the native cartilage. Here, given the potential preservation of biomechanical and biochemical cues found in the native cartilage, particulate decellularized ECM (DC‐ECM) was utilized for immobilization on the surface of nanofibrous scaffolds. Afterward, the chondro‐inductive potential and ectopic cartilage formation after subcutaneous implantation of bioengineered DC‐ECM scaffolds were investigated in mice model. Eight weeks post‐implantation, no growth of considerable inflammatory response and neovascularization was observed in histological images of bioengineered DC‐ECM scaffolds. Pre‐seeded bioengineered scaffolds with human adipose‐derived stem cells exhibited high levels of chondro‐induction capability, indicated with immunohistochemical and gene expression results. In both interval times, we also observed chondrogenesis and tissue formation after implanting unseeded bioengineered scaffolds, which denote that the presence of DC‐ECM particles can even enhance attachment and migration of the host cells and induce chondrogenesis to them. To sum up, the incorporation of DC‐ECM materials to tissue engineered constructs is a promising avenue to mimic the native tissue environment for regulation of cartilage regeneration in both in vivo and in vitro settings.

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Section: Discussionmentioning

confidence: 99%

An in vivo evaluation of induced chondrogenesis by decellularized extracellular matrix particles

Masaeli

Nasr‐Esfahani

2020

J Biomedical Materials Res

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“…Twenty‐six articles were found to involve TE applications for nasal reconstruction since 2014 (Figure 3). While the total study number is relatively small, these studies present a suitable evaluation of histologic characteristics, mechanical properties, regenerative potential, and the ability to create a 3D construct using primarily in vitro and small in vivo models 8,10‐19 . There has been an absence of testing in large animal models, however, there have been multiple attempts (four studies) to replace nasal cartilage subunits in humans 9,20‐22 …”

Section: Discussionmentioning

confidence: 99%

“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate nasal tissue engineering 8‐24,98‐106 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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“…To this aim, our group has developed a decellularized cartilage matrix from the porcine nasal septum (decellularized nasal septal cartilage–DNSC) 16,17 . The matrix has been extensively characterized for decellularization efficiency, cyto‐ and biocompatibility, as well as the suitability for operative handling in an animal model 18,19 . Furthermore, the potential of DNSC to stimulate cartilage regeneration has been demonstrated in a rabbit model 19 …”

Section: Introductionmentioning

confidence: 99%

Modulation of the inflammatory response to decellularized collagen matrix for cartilage regeneration

Gvaramia

Kern

Jakob

et al. 2021

J Biomedical Materials Res

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Decellularized extracellular matrices (DECM) are among the most common types of materials used in tissue engineering due to their cell instructive properties, biodegradability, and accessibility. Particularly in cartilage, a natural collagen type II matrix can be a promising means to provide the necessary cues and support for chondrogenic stem and progenitor cells (CSPCs). However, efficient remodeling of the transplanted DECM is largely dependent on the host immune response, with macrophages playing the central role in orchestrating both inflammatory and regenerative processes. Here we assessed the reaction of human primary macrophages to the cartilage DECM. Our findings show that the xenogeneic collagen matrix can elicit a mixed response in human macrophages, whereby the inflammatory response (M1) and the activation of remodeling (M2) type of macrophages are both present. Additionally, we demonstrate the inhibitory effect of macrophage response on the migratory capacity of human CSPCs. We further show that the inflammatory reaction of macrophages to the cartilage DECM, as well as the resulting inhibitory effects on CSPC migration, can be attenuated by interleukin‐4 (IL‐4). Finally, we demonstrate that IL‐4 can effectively bind the matrix, thereby modulating macrophage response by reducing the inflammatory reaction and inducing the M2 phenotype.

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Cartilage regeneration using decellularized cartilage matrix: Long-term comparison of subcutaneous and intranasal placement in a rabbit model

Cited by 16 publications

References 33 publications

An in vivo evaluation of induced chondrogenesis by decellularized extracellular matrix particles

An in vivo evaluation of induced chondrogenesis by decellularized extracellular matrix particles

Tissue engineering applications in otolaryngology—The state of translation

Modulation of the inflammatory response to decellularized collagen matrix for cartilage regeneration

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