Cartilage debris increases the expression of chondrodestructive tumor necrosis factor-α by articular chondrocytes

Cameron-Donaldson, Michelle; Holland, Christoph; Hungerford, David S.; Frondoza, Carmelita G.

doi:10.1016/j.arthro.2004.08.009

Cited by 17 publications

(14 citation statements)

References 15 publications

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“…This increase could be a consequence of mainly local activation of TNF production (21). Other investigators support our fi ndings (17,22).…”

Section: Discussionsupporting

confidence: 81%

The Evaluation Of Protein C Activity And Some Inflammatory Markers In Synovia Of Patients Undergoing Total Knee Arthroplasty Surgery

Alturfan¹,

Tozun²,

Emekli³

2010

TUTFD

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Objective: Total knee arthroplasty (TKA) is a major risk factor for thrombosis in patients over 40 years of age and this risk persists for several weeks after the surgery. Since infl ammatory mechanisms affect coagulation and the natural anticoagulant system, we aimed to investigate protein C activities and infl ammatory markers in patients undergoing TKA surgery. Material and Methods:We included 20 osteoarthritis patients and 20 healthy controls. Protein C activity and tumor necrosis factor-α (TNF-α) levels in plasma and synovia were evaluated by ELISA technique. Results:In the patient group, protein C activities decreased and TNF-α levels increased signifi cantly both in synovia and plasma when compared with the controls. Erythrocyte sedimentation rate of the patient group was found to be signifi cantly elevated in comparison to the controls. On the other hand, serum C reactive protein values increased insignifi cantly when compared to controls. Conclusion:The decreased activity of protein C and increased levels of infl ammatory markers in preoperative plasma and synovia of the patient group may enhance the risk for developing thrombosis.

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“…This increase could be a consequence of mainly local activation of TNF production (21). Other investigators support our fi ndings (17,22).…”

Section: Discussionsupporting

confidence: 81%

The Evaluation Of Protein C Activity And Some Inflammatory Markers In Synovia Of Patients Undergoing Total Knee Arthroplasty Surgery

Alturfan¹,

Tozun²,

Emekli³

2010

TUTFD

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“…The particulate doses (up to 1000:1 particles per cell) examined and used in this study may be compared to the range of values used for previous wear particles studies on various adult cell types involving cartilage 16 , latex 19 , polyethylene 15; 18 and titanium 14; 19; 36 that quantified dose in terms of weight/cell 16; 17 , volume percentage 14; 18; 36 and particles per cell 13; 19 . Similar to other non-biologic particles such as titanium, the viability of the synovial sheet cultures was decreased by increasing concentrations of latex particles 14 .…”

Section: Discussionmentioning

confidence: 99%

“…While the most common method of determining particle-induced effects is to apply a specific number of particles per cell 13–15 , measuring particulate mass per number of cells is also commonly used 16; 17 . Adding wear particles of any material to cells can change cellular viability, metabolic activity, function and expression of OA specific markers.…”

Section: Introductionmentioning

confidence: 99%

“…Adding wear particles of any material to cells can change cellular viability, metabolic activity, function and expression of OA specific markers. Specifically, treatment of human fibroblast-like synoviocytes (FLS) or chondrocytes with cartilage particles results in increased proteinase activity 16 and tumor necrosis factor- α (TNF-α) gene expression 17 . Lacking the availability of techniques to measure precisely the number or size of particles being used for treatment, previous studies examined biologic wear particles by mass, with an undefined size distribution.…”

Section: Introductionmentioning

confidence: 99%

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Toward understanding the role of cartilage particulates in synovial inflammation

Silverstein

Stefani

Sobczak

et al. 2017

Osteoarthritis and Cartilage

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Objective Arthroscopy with lavage and synovectomy can remove tissue debris from the joint space and the synovial lining to provide pain relief to patients with osteoarthritis (OA). Here, we developed an in vitro model to study the interaction of cartilage wear particles with fibroblast-like synoviocytes (FLS) to better understand the interplay of cartilage particulates with cytokines on cells of the synovium. Method In this study sub-10μm cartilage particles or 1μm latex particles were co-cultured with FLS ± 10 ng/mL interleukin-1α (IL-1 α) or tumor necrosis factor- α (TNF-α). Samples were analyzed for DNA, glycosaminoglycan (GAG), and collagen and media samples were analyzed for media GAG, nitric oxide (NO) and prostaglandin-E2 (PGE2). The nature of the physical interaction between the particles and FLS was determined by microscopy. Results Both latex and cartilage particles could be phagocytosed by FLS. Cartilage particles were internalized and attached to the surface of both dense monolayers and individual cells. Co-culture of FLS with cartilage particulates resulted in a significant increase in cell sheet DNA and collagen content as well as NO and PGE2 synthesis compared to control and latex treated groups. Conclusion The proliferative response of FLS to cartilage wear particles resulted in an overall increase in ECM content, analogous to the thickening of the synovial lining observed in OA patients. Understanding how cartilage particles interface with the synovium may provide insight into how this interaction contributes to OA progression and may guide the role of lavage and synovectomy for degenerative disease.

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“…All of these proteins in synovial fluid might reflect the pathological status of the knees. It has been reported that cartilage debris in the traumatized and osteoarthritic joint may increase the concentration of TNF-a in the joint, contributing to joint symptoms and cartilage destruction [20]. Interleukin-1b, like TNF-a, is a documented proinflammatory cytokine that may play an important role in the pathogenesis and progression of knee OA [21][22][23][24][25][26][27][28].…”

Section: Discussionmentioning

confidence: 99%

Role of medial abrasion phenomenon in the pathogenesis of knee osteoarthritis

et al. 2015

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Cartilage debris increases the expression of chondrodestructive tumor necrosis factor-α by articular chondrocytes

Cited by 17 publications

References 15 publications

The Evaluation Of Protein C Activity And Some Inflammatory Markers In Synovia Of Patients Undergoing Total Knee Arthroplasty Surgery

The Evaluation Of Protein C Activity And Some Inflammatory Markers In Synovia Of Patients Undergoing Total Knee Arthroplasty Surgery

Toward understanding the role of cartilage particulates in synovial inflammation

Role of medial abrasion phenomenon in the pathogenesis of knee osteoarthritis

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