Cartilage Changes Caused by a Coronal Surface Stepoff in a Rabbit Model

Lovász, György; Llinás, Adolfo; Benya, Paul D.; Park, Sang‐Hyun; Sarmiento, Augusto; Luck, James V.

doi:10.1097/00003086-199809000-00027

Cited by 25 publications

(23 citation statements)

References 27 publications

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“…Immunohistology with 3-B-3 (Seikagaku, Falmouth, Massachusetts) and 7-D-4 (kindly donated by Bruce Caterson, PhD, University of Wales, Cardiff, UK) was performed using the biotin-streptavidin-conjugated alkaline phosphatase method as described earlier. 19,21 Immunostaining with 3-B-3 was performed on sections without predigestion with chondroitinase ABC to detect the native epitope (suffix "-"). Chondroitinase-digested sections were only used as positive controls for antibody functionality.…”

Section: Methodsmentioning

confidence: 99%

“…Chondroitinase-digested sections were only used as positive controls for antibody functionality. 19,21 Qualitative description and a semiquantitative scoring system were used for evaluation of the epitopes. Scoring of 3-B-3(-) was based on the mean number of chondrocytes with detectable epitope in a high-power field (ϫ400).…”

Section: Methodsmentioning

confidence: 99%

“…Previous research on full-thickness surface offsets investigated the fate of the lesion in stable knees of rabbits. These experiments focused on the effects of the size of the defect and different methods of treatment on regeneration/degeneration of the cartilage [14][15][16][17][18][19] and have…”

mentioning

confidence: 99%

“…[15][16][17][18][19] By contrast, large defects did not heal and caused severe osteoarthritis. 14,15 Minor offsets showed qualitative changes in the immunohistological detection of 3-B-3(-) and 7-D-4 chondroitin sulphate epitopes associated with the remodelling and adaptation which occurred around the defect.…”

mentioning

confidence: 99%

“…14,15 Minor offsets showed qualitative changes in the immunohistological detection of 3-B-3(-) and 7-D-4 chondroitin sulphate epitopes associated with the remodelling and adaptation which occurred around the defect. 18,19 We have investigated the effect of ligamentous instability on the fate of a minor coronal step-off in an established rabbit model of intra-articular incongruity.…”

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confidence: 99%

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Characteristics of degeneration in an unstable knee with a coronal surface step-off

Lovász

Park²,

Ebramzadeh

et al. 2001

The Journal of Bone and Joint Surgery. British volume

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T o investigate the effect of instability on the remodelling of a minor articular surface offset, we created a 0.5 mm coronal step-off of the medial femoral condyle in 12 New Zealand white rabbits and transected the anterior cruciate ligament (ACL). A control group of 12 rabbits had only ACL resection and the opposite knee was used as the non-operated control. The osteoarthritic changes at 6, 12 and 24 weeks after surgery were evaluated histologically. In addition, changes in the immunological detection of 3-B-3(-) and 7-D-4 chondroitin-6-sulphate epitopes were determined because of the previous association of such changes with repair of cartilage and early osteoarthritis.In the instability/step-off group there was rapidly progressing focal degeneration of cartilage on the high side of the defect, not seen in previous step-off studies in stable knees. The rest of the femoral condyles and the tibial plateaux of the instability/step-off group had moderate osteoarthritis similar to that of the instability group. 3-B-3(-) was detectable in the early and the intermediate stages of osteoarthritis but no staining was seen in the severely damaged cartilage zones. Immunoreactivity with 7-D-4 increased as degeneration progressed.Our findings have shown that even a minor surface offset may induce rapid degeneration of cartilage when the stability of the knee is compromised.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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