Carry-over of thermophilic Campylobacter spp. between sequential and adjacent poultry flocks

Alter, Thomas; Weber, Rita; Hamedy, Ahmad; Glünder, G

doi:10.1016/j.vetmic.2010.06.005

Cited by 25 publications

(28 citation statements)

References 22 publications

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“…Multiple genotypes can constitute the Campylobacter population within poultry flocks, suggesting different sources of exposure and/or genetic drifts within the Campylobacter population [4].…”

Section: Discussionmentioning

confidence: 99%

“…Advanced molecular typing tools have improved our understanding of the epidemiology of bacterial food-borne pathogens. Monitoring of poultry flocks has shown that some are infected with only one genotype of Campylobacter spp., while more than one genotype has been detected in others [4], [5]. Different strains in individual flocks may be replaced or displaced by others during the rearing cycle [6], [7].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Genetic Diversity in Campylobacter jejuni Isolated from a Commercial Turkey Flock Using flaA Typing, MLST Analysis and Microarray Assay

et al. 2013

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Campylobacter is genetically highly diverse and undergoes frequent intraspecific recombination. Turkeys have been identified as an important reservoir for Campylobacter jejuni which is of public health significance. The assessment of the genetic diversity among Campylobacter population is critical for our understanding of the epidemiology of this bacterium. The genetic profiles were different according to the molecular typing methods used. The performance of established flaA genotyping, multilocus sequencing typing (MLST) and DNA microarray assay based on the ArrayTube™ technology was evaluated using 14 Campylobacter jejuni isolated from a commercial turkey flock. The flaA typing was performed using PCR-RFLP with restriction enzymes Sau3AI, AluI, a ‘composite’ flaA analysis of AluI and Sau3AI and DdeI. The 14 isolates were differentiated into 3, 5, 7 and 9 genotypes, respectively. Entire flaA gene and short variable region (SVR) sequences were analysed. Sequencing of the entire flaA provided 11 different genotypes. flaA-SVR sequence analysis detected 8 flaA alleles and 4 flaA peptides. One new flaA allele type (528) was identified. MLST analysis represented 10 different sequence types (STs) and 5 clonal complexes (CCs). The microarray assay recognised 14 different genotypes. The discriminatory indices were 0.560, 0.802, 0.857, and 0.912 for flaA-RFLP depending on the used enzymes, 0.890 for flaA-SVR, 0.967 for entire flaA sequencing, 0.945 for MLST and 1.00 for the DNA microarray assay. The flaA gene was genetically stable over 20 passages on blood agar. In conclusion, the different typing tools demonstrated a high level of genetic heterogeneity of Campylobacter jejuni in a turkey flock, indicating that a single flock can be infected by multiple genotypes within one rearing cycle. DNA microarray-based assays had the highest discriminatory power when compared with other genotyping tools.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Genetic Diversity in Campylobacter jejuni Isolated from a Commercial Turkey Flock Using flaA Typing, MLST Analysis and Microarray Assay

et al. 2013

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“…This diversity should be considered when evaluating microbial risk assessments. Multiple genotypes of Campylobacter populations can be present within a flock, which could reduce the effectiveness of the applied phage (Alter et al, 2011). Campylobacter strains may also develop resistance to pre-harvest applied phages (Atterbury et al, 2003;Fischer et al, 2013).…”

Section: Bacteriophage Application In Poultry Production and Processingmentioning

confidence: 99%

Salmonella and Campylobacter : Antimicrobial resistance and bacteriophage control in poultry

2016

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“…So können in entsprechend belasteten Masthühnerher-den 50 bis 100% der Einzeltiere Campylobacter-positiv sein [5]. Interessanterweise ist der Anteil von C. coli an den isolierten Campylobacter-Arten in Putenherden mit 46% deutlich höher als bei Masthüh-nern mit 11% [6]. Es kann vermutet werden, dass in Putenherden mit langer Haltungsperiode C. coli im Verlauf der Zeit C. jejuni verdrängt, sodass sich das Infektionsrisiko für den Menschen in Richtung C. coli verändert.…”

Section: Reservoirunclassified

“…Dennoch konnten in den meisten Herden bis zu 14 verschiedene Genotypen nachgewiesen werden. Dies weist auf einen intensiven Erregeraustausch zwischen den Herden und der Umwelt sowie zwischen den Herden selbst hin [6].…”

Section: Reservoirunclassified

Die Campylobacteriose des Menschen

Alter

Bereswill²,

Glünder³

et al. 2011

Bundesgesundheitsbl.

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Over the last few years, infections with Campylobacter have significantly increased in Europe and Germany and these bacteria have even surpassed Salmonella as the most prevalent bacteria, causing gastroenteritis. Especially contamination during the handling and consumption of meat products seems to be the most important risk factor which plays a prominent role for transmission to man. In addition, contact with pets and other animals, drinking raw or improperly pasteurized milk, and the tenacity of Campylobacter in different environments, especially water, have also to be considered for an adequate risk assessment. Besides gastroenteritis, arthralgia, and Guillain-Barré syndrome are important clinical complications of Campylobacter infections in man. At the same time, it is mostly unclear why the course of infection in man and in reservoir animals differs significantly, especially as only a few classical bacterial virulence factors have been identified so far. For these reasons, the development of efficient prevention strategies is of utmost importance in order to control campylobacteriosis.

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Carry-over of thermophilic Campylobacter spp. between sequential and adjacent poultry flocks

Cited by 25 publications

References 22 publications

Detection of Genetic Diversity in Campylobacter jejuni Isolated from a Commercial Turkey Flock Using flaA Typing, MLST Analysis and Microarray Assay

Detection of Genetic Diversity in Campylobacter jejuni Isolated from a Commercial Turkey Flock Using flaA Typing, MLST Analysis and Microarray Assay

Salmonella and Campylobacter : Antimicrobial resistance and bacteriophage control in poultry

Die Campylobacteriose des Menschen

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