Carrot Doubled Haploids

Górecka, Krystyna; Krzyżanowska, D.; Kiszczak, Waldemar; Kowalska, Urszula; Górecki, Ryszard J.

doi:10.1007/978-1-4020-8854-4_20

Cited by 12 publications

(11 citation statements)

References 8 publications

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“…Based on the results, it was ascertained that bud length was correlated with the developmental stage of microspores, and was therefore a good indicator. For many plants, the uninuclear stage is the most suitable for stimulating the development of androgenetic androgenic embryos (Górecka et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)

Górecka

Krzyżanowska

Kowalska

et al. 2017

JCEA

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IAA (indole-3-acetic acid). First androgenic embryos were placed on B 5 medium without plant growth regulators and then on MS medium containing 0.2 mg·L -1 BAP and 1 mg·L -1 NAA (α-naphthaleneacetic acid). Androgenic embryos died on B 5 regeneration medium without plant growth regulators. On MS medium first shoots and callus with and without roots were obtained. Rosettes withered during following passages whereas callus tissue developed further. The quantity of DNA in this tissue equivalent to 4X chromosomes.

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Section: Resultsmentioning

confidence: 99%

Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)

Górecka

Krzyżanowska

Kowalska

et al. 2017

JCEA

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“…Currently, the list of cultivated species that have originated from haploids derived through in vitro androgenesis includes barley [58], wheat [59], rape [62], carrot [65][66][67], cucumber [68] and cabbage [69]. Production of embryos from female gametophyte cells via apogamy or parthenogenesis ensures stability of DH lines (while retaining a low rate of albinism).…”

Section: Haploids and Double Haploid Plantsmentioning

confidence: 99%

65 years of in vitro culture in Poland

Zenkteler

2013

Acta Soc Bot Pol

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This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements

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“…Andersen et al (1990) and Tyukavin et al (1999) studied the ploidy of plants obtained from anther cultures by counting chromosomes of the meristematic cells of root tips. However, in the literature pertaining to androgenesis in carrot, there is only one example of the use of flow cytometry for determining the ploidy of androgenetic plants (Górecka et al 2009a). Among the aforementioned researchers studying androgenesis in carrot, Tyukavin et al (1999) tested the plants obtained from anther cultures in terms of homozygosity.…”

Section: Introductionmentioning

confidence: 98%

“…sativus, and the relationships between loci of those isoenzymes. Górecka et al (2009a) showed the use of PGI and AAT isoenzymatic markers for testing the homozygosity of carrot androgenetic plants. PGI and AAT are two important enzymes, which occur in glycolysis and amino acid metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Determination of ploidy and homozygosity of carrot plants obtained from anther cultures

Kiszczak¹,

Krzyżanowska²,

Strycharczuk

et al. 2010

Acta Physiol Plant

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The purpose of the present work was to evaluate carrot plants obtained from anther cultures with respect to their ploidy and homozygosity. Ploidy was determined using flow cytometry. Homozygosity of analyzed plants was determined using isoenzymes, glucose-6-phosphate isomerase (PGI) and aspartate aminotransferase (AAT). The cytometric tests revealed that more than 90% of the carrot plants obtained from anther cultures were doubled haploid. In the initial assessment of polymorphism of the two enzymatic systems in selected androgenetic carrot plants of the cultivars: Berjo, Kazan F 1 , and Splendid F 1 , it was proven that 100% of those plants were homozygotes in respect to PGI and also with respect to AAT. In the second experiment, the obtained androgenetic progeny of the heterozygous donor plant of cultivar Narbonne F 1 was found to be 94% homozygotic with respect to PGI and 100% homozygotic in the case of AAT. For the androgenetic plants of the cultivar Kazan F 1 , 89% of them were homozygotic with respect to AAT but PGI enzyme system did not differentiate the homozygotic and heterozygotic androgenetic progeny. These results indicated that enzyme polymorphism depends on carrot genotype, therefore, analysis of some (more than one) isozymes was necessary to confirm homozygosity of plants. PGI and AAT can be a useful tool for determining homozygosity in androgenetic carrot plants.

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Carrot Doubled Haploids

Cited by 12 publications

References 8 publications

Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)

Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)

65 years of in vitro culture in Poland

Determination of ploidy and homozygosity of carrot plants obtained from anther cultures

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