Carrot

Bradeen, James M.; Simon, Philipp W.

doi:10.1007/978-3-540-34536-7_4

Cited by 12 publications

(5 citation statements)

References 110 publications

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“…Utilization of heterosis has been the main focus in carrot breeding since the discovery of genic-cytoplasmic male sterility (CMS; Peterson and Simon 1986;Bradeen and Simon 2007), but a lack of homozygous parents and a long breeding period highlight the potential for deriving hybrids. With DH production systems established, homozygous lines can be achieved in one generation, which would allow to reduce the time and cost of cultivar development (Dunwell 2010;Ferrie and Caswell 2010;Germanà 2011).…”

Section: Discussionmentioning

confidence: 99%

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Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Zhuang

et al. 2012

Plant Cell Tiss Organ Cult

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Protocols were developed for the generation of haploid and doubled haploid plants from isolated microspores of carrot (Daucus carota L.). Forty-seven carrot accessions, including six inbred lines, 11 cultivars, 20 F 1 s, two BC 1 F 1 s, four F 2 s, one F 3 , and three F 4 s, were screened to evaluate the genotype influence on isolated microspore embryogenesis over 4 years. Twenty-eight accessions responded by producing embryos and/or calli. A cytological analysis showed that two modes of carrot microspore embryogenesis exist: an indirect route via calli (C mode), and a direct route via embryos (E mode). Eleven accessions were in the C mode, and 17 were in both modes. The highest production rates were in 10Y25 (a European Nantes cultivar) with 27 calli and 307 embryos, and 100Q6 (a semi-Nantes F 1 hybrid) with 176 calli and 114 embryos. The time period to produce embryos or calli differed significantly between 2 and 6 months. Cold and heat pretreatment generally had a negative impact on the induction of microspore embryogenesis, but a short pretreatment showed a positive influence on some accessions. Twentyeight lines regenerated plants from the primary individual embryos or calli of three accessions were established to analyze the ploidy level. The percentage of spontaneous diploidization showed very wide differences among the accessions and lines. Differences in leaf color intensity, leaf size, and leaf dissection were found among haploid, doubled haploid, and triploid plants.

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Section: Discussionmentioning

confidence: 99%

“…Its flowering is initiated after a vernalization period of eight to 10 weeks and the crop is typically bred in an annual cycle (Bradeen and Simon 2007). Selection and production of inbred lines is time consuming and difficult to achieve.…”

Section: Introductionmentioning

confidence: 99%

Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Zhuang

et al. 2012

Plant Cell Tiss Organ Cult

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“…The time for vernalization must be at least 6 weeks. However, some wild carrots will flower or bolt with little or no vernalization 15,16 .…”

Section: Biology and Originsmentioning

confidence: 99%

Advances in research on the carrot, an important root vegetable in the Apiaceae family

et al. 2019

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Carrots ( Daucus carota L.), among the most important root vegetables in the Apiaceae family, are cultivated worldwide. The storage root is widely utilized due to its richness in carotenoids, anthocyanins, dietary fiber, vitamins and other nutrients. Carrot extracts, which serve as sources of antioxidants, have important functions in preventing many diseases. The biosynthesis, metabolism, and medicinal properties of carotenoids in carrots have been widely studied. Research on hormone regulation in the growth and development of carrots has also been widely performed. Recently, with the development of high-throughput sequencing technology, many efficient tools have been adopted in carrot research. A large amount of sequence data has been produced and applied to improve carrot breeding. A genome editing system based on CRISPR/Cas9 was also constructed for carrot research. In this review, we will briefly summarize the origins, genetic breeding, resistance breeding, genome editing, omics research, hormone regulation, and nutritional composition of carrots. Perspectives about future research work on carrots are also briefly provided.

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“…Phenotypic and molecular diversity of carrot is expansive [ 5 ] and this diversity has been important in improving nutritional value and consumer quality; disease and pest resistance; and yield characteristics important for growers. Carrot genetic linkage maps have primarily been developed with anonymous AFLPs and RAPDs that require no prior genomic information, but these techniques yield primarily dominant markers [ 6 ]. Sequence-tagged codominant markers have been developed to facilitate selection for several traits, including major genes for carotenoid accumulation [ 7 ], sugar type [ 8 ], root-knot nematode resistance [ 9 ], and for 22 genes in the carotenoid biosynthetic pathway targeted for candidate gene analysis [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

De novo assembly and characterization of the carrot transcriptome reveals novel genes, new markers, and genetic diversity

et al. 2011

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BackgroundAmong next generation sequence technologies, platforms such as Illumina and SOLiD produce short reads but with higher coverage and lower cost per sequenced nucleotide than 454 or Sanger. A challenge now is to develop efficient strategies to use short-read length platforms for de novo assembly and marker development. The scope of this study was to develop a de novo assembly of carrot ESTs from multiple genotypes using the Illumina platform, and to identify polymorphisms.ResultsA de novo assembly of transcriptome sequence from four genetic backgrounds produced 58,751 contigs and singletons. Over 50% of these assembled sequences were annotated allowing detection of transposable elements and new carrot anthocyanin genes. Presence of multiple genetic backgrounds in our assembly allowed the identification of 114 computationally polymorphic SSRs, and 20,058 SNPs at a depth of coverage of 20× or more. Polymorphisms were predominantly between inbred lines except for the cultivated x wild RIL pool which had high intra-sample polymorphism. About 90% and 88% of tested SSR and SNP primers amplified a product, of which 70% and 46%, respectively, were of the expected size. Out of verified SSR and SNP markers 84% and 82% were polymorphic. About 25% of SNPs genotyped were polymorphic in two diverse mapping populations.ConclusionsThis study confirmed the potential of short read platforms for de novo EST assembly and identification of genetic polymorphisms in carrot. In addition we produced the first large-scale transcriptome of carrot, a species lacking genomic resources.

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Carrot

Cited by 12 publications

References 110 publications

Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Advances in research on the carrot, an important root vegetable in the Apiaceae family

De novo assembly and characterization of the carrot transcriptome reveals novel genes, new markers, and genetic diversity

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