Carrier membrane as a stationary phase for affinity chromatography and kinetic studies of membrane-bound enzymes

Abou-Rebyeh, H.; Körber, Friedrich; Schubert-Rehberg, K.; Reusch, Joachim; Josić, Dj.

doi:10.1016/0378-4347(91)80250-g

Cited by 88 publications

(49 citation statements)

References 12 publications

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“…In 1991 AbouRebyeh at al. reported on the use of affinity disks to isolate carbonic anhydrase from haemolysates of human erythrocytes [16]. Recovery and biological activity of the product were similar to that achieved with conventional columns.…”

Section: Isolation Of Proteins From Complex Mixturesmentioning

confidence: 71%

“…For "chromatographic" applications it was desirable to insert the sheets into dedicated flow systems. The first systems suffered from problems with handling the liquid phase and pronounced sample dilution [3,15,16]. In order to use the entire membrane/monolith surface for adsorption, the incoming flow had to be distributed over a wide area and later collected again for detection.…”

Section: From Interactive Membranes To "Membranementioning

confidence: 99%

See 1 more Smart Citation

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Tennikova

Freitag

2000

J. High Resol. Chromatogr.

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Section: Isolation Of Proteins From Complex Mixturesmentioning

confidence: 71%

Section: From Interactive Membranes To "Membranementioning

confidence: 99%

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Tennikova

Freitag

2000

J. High Resol. Chromatogr.

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“…For example, carbonic anhydrase that was immobilized in a GMA/EDMA membrane was found to have activities of 64 -68% versus the soluble form of this enzyme when used with the substrates 4-nitrophenyl acetate and 2-chloro-4-nitrophenyl acetate [43] lized in monolithic GMA/EDMA rods and beads, which were then compared to traditional media in their use within enzyme reactors and affinity columns. It was found in this case that the performance of this monolith as an enzyme reactor was substantially better than that seen when using trypsin on GMA/EDMA beads, an effect believed to be due to enhanced mass transfer in the monoliths [79].…”

Section: Bioaffinity Chromatographymentioning

confidence: 99%

Affinity monolith chromatography

Mallik

Hage²

2006

J of Separation Science

189

282

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The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method. Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes. Several reported applications will then be presented, such as the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, and biomimetic chromatography. Other applications that will be discussed are chiral separations and studies of biological interactions based on AMC.

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“…Interestingly, one of the very early applications of poly(glycidyl methacrylate-co-ethylene dimethacrylate) polymers just targeted immobilization of trypsin [54]. Since the first monoliths developed for separations also comprised glycidyl methacrylate units [13], it is no wonder that these monoliths were very early on used for immobilization of proteins [55].…”

Section: Glycidyl Methacrylate Copolymersmentioning

confidence: 99%

Less common applications of monoliths: I. Microscale protein mapping with proteolytic enzymes immobilized on monolithic supports

Švec

2006

Electrophoresis

131

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This review summarizes the recent contributions to the rapidly growing area of immobilized enzymes employing both silica and synthetic polymer-based monoliths as supports. Focus is mainly on immobilized proteolytic enzyme reactors designed for studies in proteomics. Porous monoliths emerged first as a new class of stationary phases for HPLC in the early 1990s. Soon thereafter, they were also used as supports for immobilization of proteins and preparation of both stationary phases for bioaffinity chromatography and enzymatic reactors. Organic polymer-based monoliths are typically prepared using a simple molding process carried out within the confines of a "mold" such as chromatographic column or capillary. Polymerization of a mixture comprising monomers, initiator, and porogenic solvent affords macroporous materials. In contrast, silica-based monoliths are first formed as a rigid rod from tetraalkoxysilane in the presence of PEG and subsequently encased with a plastic tube. Both types of monolith feature large through-pores that enable a rapid flow-through. Since all the solutions must flow through the monolith, the convection considerably accelerates mass transfer within the monolith. As a result, reactors including enzyme immobilized on monolithic support exhibit much higher activity compared to the reactions in solution.

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Carrier membrane as a stationary phase for affinity chromatography and kinetic studies of membrane-bound enzymes

Cited by 88 publications

References 12 publications

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Affinity monolith chromatography

Less common applications of monoliths: I. Microscale protein mapping with proteolytic enzymes immobilized on monolithic supports

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