2021
DOI: 10.1016/j.bioactmat.2021.04.027
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Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma

Abstract: Nanosuspensions, as a new drug delivery system for insoluble drugs, are only composed of a drug and a small amount of stabilizer, which is dispersed in an aqueous solution with high drug-loading, small particle size, high dispersion, and large specific surface area. It can significantly improve the dissolution, bioavailability, and efficacy of insoluble drugs. In this study, paclitaxel nanosuspensions ((PTX)NS) were prepared by an ultrasonic precipitation method, with the characteristics of simple preparation … Show more

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Cited by 74 publications
(52 citation statements)
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“…The biodistribution and uptake of nanoparticles by cells can determine their therapeutic efficacy and toxicity in vivo [ [46] , [47] , [48] ] . In previous reports, the interaction of synthetic nanoparticles with cells and their distribution in the blood circulation system were well investigated in vitro cell experiments and some animal models [ 49 , 50 ].…”
Section: Discussionmentioning
confidence: 99%
“…The biodistribution and uptake of nanoparticles by cells can determine their therapeutic efficacy and toxicity in vivo [ [46] , [47] , [48] ] . In previous reports, the interaction of synthetic nanoparticles with cells and their distribution in the blood circulation system were well investigated in vitro cell experiments and some animal models [ 49 , 50 ].…”
Section: Discussionmentioning
confidence: 99%
“…An intracranial glioma-bearing mouse model was established according to previously described procedures [ 46 ]. After anesthetizing C6 cells with chloral hydrate (4%, w/v%), 2 μL of the cells at a density of 2 × 10 6 were injected into the right striatum (1.8 mm lateral, 1 mm longitudinal, and 4 mm depth) of ICR mice.…”
Section: Methodsmentioning
confidence: 99%
“…To synthesize the Aβ-CN-modified targeted carrier, the Aβ-CN peptide with an additional cysteine in its N-terminus was coupled to the terminal mal groups of Mal-PEG 2000 –PLA 1300 using the Michael addition reaction, as described in the previous section [ 29 31 ]. First, Mal-PEG 2000 –PLA 1300 was mixed with an aqueous solution of Aβ-CN to form a suspension.…”
Section: Methodsmentioning
confidence: 99%
“…Two days later, the apical culture medium was replaced and HUVECs were incubated with free Cou-6, Cou-6/PMs, Cou-6/Aβ-CN-PMs, and rhApoE/Cou-6/Aβ-CN-PMs for 3 h. After incubation, the C6 cells in the lower chamber were washed with cold PBS thrice and fixed with 4% paraformaldehyde for 15 min. The C6 cell nuclei were then stained with DAPI, and the BBTB penetration and targeting ability of Aβ-CN-PMs were analyzed using CLSM [ 3 , 29 , 36 ]. For quantitative analysis, the fluorescence intensity of the C6 cells was determined through flow cytometry using the same experimental steps as described above in “ Cellular uptake assays ” section.…”
Section: Methodsmentioning
confidence: 99%