Carrier-free Cultured Autologous Oral Mucosa Epithelial Cell Sheet (CAOMECS) for Corneal Epithelium Reconstruction: A Histological Study

Bardag‐Gorce, Fawzia; Oliva, Joan; Wood, A. K. W.; Hoft, Richard H.; Pan, Derek; Thropay, Jacquelyn; Makalinao, Andrew; French, Samuel W.; Niihara, Yutaka

doi:10.1016/j.jtos.2014.12.003

Cited by 39 publications

(60 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell sheet technology therefore provides several advantages over traditional tissue engineering. A large and growing body of literature has investigated the possibility of applying cell sheets to cardiac regeneration [1], cornea [2], esophagus [3], periodontal tissue [4], and dental implant [5]. Thus far, cell sheet technology is an area of research that is of great interest for tissue engineering and regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Jiang

Lai

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.

show abstract

Section: Introductionmentioning

confidence: 99%

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Jiang

Lai

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Little is known about cell sheet vitrification; more studies are needed to increase our knowledge and to improve the vitrification methodology of cell sheets. In our study, we showed that CAOMECS stored in liquid nitrogen, with vitrification solutions, broke during the thawing process, thus making them not suitable for harvesting nor for cornea transplantation (Bardag‐Gorce et al, ; Burillon et al, ; Hayashida et al, ; Nishida et al, ). For this reason, we focused on analyzing CAOMECS stored in the absence of vitrification solutions.…”

Section: Discussionmentioning

confidence: 94%

“…RNA sequencing, combined with mass spectrometry, will be the best approach to compare the transcriptome and proteins before and after liquid nitrogen storage. Additionally, the functionality of vitrified CAOMECS needs to be tested in the future to ensure that vitrified CAOMECS can reverse the LSCD on animal models (Bardag‐Gorce et al, ; Sugiyama et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…For the past few years, we have been developing multilayer CAOMECS by growing isolated oral mucosal epithelial cells to reverse the limbal stem cell deficient (LSCD) of a rabbit model (Bardag‐Gorce et al, ). Cell sheets are engineered in the laboratory and transported to the surgery room, where they are then transplanted on the rabbit LSCD cornea (Bardag‐Gorce et al, ). The corneal epithelium is renewed by the migration of epithelial cells, coming from the limbus: the limbal stem cells (Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vitrification and storage of oral mucosa epithelial cell sheets

Oliva

Florentino

Bardag‐Gorce

et al. 2019

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

Shipping time and shipping delays might affect the quality of the stem cells based engineered “organs.” In our laboratory, we have developed a limbal stem cell deficient (LSCD) rabbit model. To reverse the LSCD, we cultured oral mucosal epithelial cells for 2–3 weeks and engineered cultured autologous oral mucosa epithelial cell sheets (CAOMECS), which were grafted on the LSCD cornea. The purpose of this study was to vitrify CAOMECS and to store it until the CAOMECS can be grafted onto patients. CAOMECS were vitrified in LN2 for up to 204 days. We tested two different methods of vitrification with different solutions; however, CAOMECS were only viable when they were not stored in a vitrification solution; results were only reported from this CAOMECS. On the basis of hematoxylin and eosin staining, we showed that the CAOMECS morphology was well preserved after long‐term storage in LN2. Most of the preservation solutions maintained the CAOMECS phenotype (Ki67, proliferating cell nuclear antigen (PCNA), Beta‐Catenin, ZO‐1, E‐Cadherin, CK3, CK4, CK13). The exception was the solution composed with ethylene glycol and Dimethyl sulfoxide (DMSO): this resulted in loss of DeltaN‐p63 expression. DeltaN‐p63 is an important marker for cell proliferation. The expression of proteins involved in cell–cell connection and the differentiation markers were maintained. Apoptosis was not detected in the thawed CAOMECS. We demonstrated that CAOMECS can be stored long‐term in LN2 without affecting their morphology and phenotype.

show abstract

“…Meanwhile, studies have demonstrated that the tracheal epithelial-cell sheets can create a sufficient epithelial lining on the luminal surface of a bioartificial trachea in in vivo experiments. In addition, cell sheets of other tissues have been used for various types of transplantation and regeneration, such as corneal epithelium reconstruction and ear or esophageal-mucosal regeneration [160][161][162]. However, major disadvantages of the cell-sheet methodology are the time-consuming procedure required to create a robust structure for transplantation and the difficulties associated with handling the sheets.…”

Section: Epithelial Cell Sheet Engineeringmentioning

confidence: 99%

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

Zhang

2015

Regen. Med.

View full text Add to dashboard Cite

Trachea-tissue engineering is a thriving new field in regenerative medicine that is reaching maturity and yielding numerous promising results. In view of the crucial role that the epithelium plays in the trachea, re-epithelialization of tracheal substitutes has gradually emerged as the focus of studies in tissue-engineered trachea. Recent progress in our understanding of stem cell biology, growth factor interactions and transplantation immunobiology offer the prospect of optimization of a tissue-engineered tracheal epithelium. In addition, advances in cell culture technology and successful applications of clinical transplantation are opening up new avenues for the construction of a tissue-engineered tracheal epithelium. Therefore, this review summarizes current advances, unresolved obstacles and future directions in the reconstruction of a tissue-engineered tracheal epithelium.

show abstract

Carrier-free Cultured Autologous Oral Mucosa Epithelial Cell Sheet (CAOMECS) for Corneal Epithelium Reconstruction: A Histological Study

Cited by 39 publications

References 23 publications

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

Vitrification and storage of oral mucosa epithelial cell sheets

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

Contact Info

Product

Resources

About