Carrier-based Preparations of Plant Growth-promoting Bacterial Inoculants Suitable for use in Cooler Regions

Abstract

Carrier-based preparations of two plant growth-promoting rhizobacteria (PGPR) viz. Bacillus subtilis and Pseudomonas corrugata, developed in five formulations were evaluated for their growth promotion, rhizosphere colonization, and viability under storage. The effect of these formulations as fresh preparations, and after 6 months of storage at 4°C and room temperature, was also determined. The bacterial inoculants in all the formulations were found to enhance the growth parameters of the test plant species; best results were obtained in case of alginate-based formulations. Maximum numbers of inoculated bacteria were recovered from the rhizosphere of alginate-based formulation-treated plants after 6 weeks of growth. Viability of bacterial inoculants was maximal in alginate beads, and alginate beads supplemented with skim milk formulations, after 180 days of storage at 4°C.

Plant growth promotion abilities and formulation of Bacillus megaterium strain B 388 (MTCC6521) isolated from a temperate Himalayan location

“…The decrease in the formulations was minimum when alginate beads were used. Alginate has been known to preserve the benefi cial properties and cell numbers of PGPR(s) for longer period of time [19] and its superiority over other carrier materials in maintaining the viability has been described previously [12]. Studies in various ecological niches have indicated that the relative proportion of different microbes and dominance of a particular group of microorganism (species or strains) depend upon various environmental factors.…”

“…Three formulations (broth, coal and alginate beads) of the bacterium were prepared following the procedure described in Trivedi et al [12]. TY broth containing 10 9 cfu/ml was directly used as broth formulation.…”

Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas

“…The beneficial effects of plant growth promoting rhizobacteria, particularly those belonging to the genus Bacillus or Pseudomonas, in enhancing growth and overall plant establishment is well Several species of Pseudomonas that produce secondary metabolites, mobilize nutrients, and promote plant growth have been reported from Indian Himalayan region (Kumar et al, 2005;Pandey et al, 2006;Trivedi et al, 2005, Selvakumar et al 2009). …”

Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)

“…Alginate formulation supported high populations and survival of the phosphate-solubilizing bacteria Pseudomonas striata and Bacillus polymyxa at the elevated storage temperature of 40°C (Viveganandan and Jauhri 2000). Several alginate formulations (macrobeads with and without skim milk and seed coating) of B. subtilis and Pseudomonas corrugata were found superior over liquid inoculants or charcoal-based inoculants for inoculating maize plants under low temperatures in the Indian Himalayas (Trivedi et al 2005).…”