Carriage of complement regulatory proteins by vesicles (prostasomes) in seminal plasma

Rooney,

doi:10.1016/0161-5890(93)90349-g

Cited by 7 publications

(3 citation statements)

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“…SP CD55 is unlikely to arise from testis (which expresses CD55 of lower molecular mass than the 70-and 76-kD species identified here [22,25]) or from prostate, which expresses CD55 very poorly (22); Therefore, likely sites of CD55 production are epididymis and seminal vesicles. CDw52 was absent from VSP, indicating that this protein arises proximal to the point of transection, most likely from the epididymis, where it is very strongly expressed (5).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells.

Rooney¹,

Heuser²,

Atkinson³

1996

J. Clin. Invest.

140

104

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We analyzed and compared the properties of three glycosylphosphatidylinositol (GPI)-anchored proteins, CD59, CD55 (both C regulators), and CDw52, and of the transmembrane C regulator CD46 in seminal plasma (SP). We demonstrated previously that anchor-intact SP CD59 is present on the membranes of vesicles (prostasomes) and that cells acquire this protein during incubation with SP. We now report that this acquisition is due partly to adherence of prostasomes to cells and partly to a second mechanism which may involve micellar intermediates. Using fluorescent labeling, ultracentrifugation, and density gradient centrifugation, virtually all CD46 was present on prostasomes whereas CD59, CD55, and CDw52 were also detected in a form which remained in the 200,000 g supernatant and equilibrated at higher density than prostasomes in gradients. All three GPI-linked proteins eluted at high molecular mass during size exclusion chromatography of this nonprostasome

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Section: Discussionmentioning

confidence: 99%

“…In addition to CD59, we studied two other GPI-anchored proteins, CD55 and CDw52, and a transmembrane protein, CD46, all of which have been identified in SP (3)(4)(5)(6). CD55 (decay accelerating factor) and CD46 (membrane cofactor protein) are inhibitors of C activation (for review see reference 7).…”

Section: Introductionmentioning

confidence: 99%

GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells.

Rooney¹,

Heuser²,

Atkinson³

1996

J. Clin. Invest.

140

104

View full text Add to dashboard Cite

show abstract

“…The GPI anchors contain a linear ethanolamine (EthN-P-ManManManGlcN) core sequences that are variably decorated in different organisms and the biosynthesis of which has started to be elucidated (33), although the enzymes involved have not been purified yet. Prostasomes, which contain at least three GPI-proteins (CD59; DAF; CDw52) have been considered to cany and to provide a reservoir of these proteins (131). In this context it could be anticipated that prostasomes may contain several other GPI proteins such as folate binding protein which is present in human seminal plasma with its GPI anchor (42).…”

Section: Gpi-anchored Membrane Proteinsmentioning

confidence: 99%