Carriage of Class 1 and 2 Integrons in Quinolone, Extended-Spectrum-β-Lactamase-Producing and Multi Drug Resistant E.coli and K.pneumoniae: High Burden of Antibiotic Resistance

Shams, Fariza; Hasani, Akbar; Rezaee, Mohammad Ahangarzadeh; Mr, Nahaie; Mh, Soroush; Pormohammad, Ali; A, Elli Arbatan

doi:10.15171/apb.2015.047

Cited by 14 publications

(6 citation statements)

References 40 publications

(37 reference statements)

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“…However, the slowing of upward trend seems to suggest that the appropriate use of antibiotics is proceeding. Shams et al reported that ESBL production was found in 58.3% and 69.8% of FQ-resistant E. coli and K. pneumoniae, respectively, and class 1 and 2 integrons were found in 73.5% of MDR isolates [27]. Awareness of the risk of emergence of MDR E. coli should be raised.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the susceptibility trends in Japan to fluoroquinolones and other antimicrobial agents in a nationwide collection of clinical isolates: A longitudinal analysis from 1994 to 2016

Tateda

Ohno

Ishii

et al. 2019

Journal of Infection and Chemotherapy

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The susceptibilities of clinical isolates to fluoroquinolones and other antimicrobial agents were surveyed to obtain an accurate understanding of trends in incidence and antimicrobial resistance. The samples were collected from across Japan, biennially or triennially, between 1994 and 2016 and a defined level of resistance to fluoroquinolone was determined. Streptococcus pneumoniae, Streptococcus pyogenes and Haemophilus influenzae exhibited stable and high rates of susceptibility to fluoroquinolones over the period examined. For methicillin-resistant Staphylococcus aureus the rate of resistance to levofloxacin and ciprofloxacin was 81.3e93.5% and 83.2e94.2%, respectively, which was markedly higher than that of methicillin-susceptible S. aureus, while sitafloxacin-resistant methicillin-susceptible and methicillin-resistant S. aureus were isolated at 0.3e0.7% and 16.9e36.5%, respectively. The rate of levofloxacin or ciprofloxacin-resistant Escherichia coli increased from around 2e3% between 1994 and 1998 to around 35% in 2016, but the rate of fluoroquinolonesusceptible Klebsiella pneumoniae stayed high at over 94.6% during the study period. Although no fluoroquinolone-resistance in clinical isolates of Salmonella spp. was detected from 1994 to 2002, the resistance rate increased slightly after 2004 and reached to 1.9%e4.7% in 2016. The rate of fluoroquinolone-susceptible Pseudomonas aeruginosa isolated from urinary tract and respiratory tract infections improved during the period examined from 41.8e67.0% to 91.2e94.2%, and from 78.9-88.5% to 90.1e94.6%, respectively. Against Acinetobacter spp., the susceptibility rate of fluoroquinolones was almost constant at around 90%, but one multidrug-resistant isolate was detected in 2013. Overall, the susceptibility to fluoroquinolones was maintained over 20 years against tested bacteria except for MRSA and E. coli.

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Section: Discussionmentioning

confidence: 99%

Investigation of the susceptibility trends in Japan to fluoroquinolones and other antimicrobial agents in a nationwide collection of clinical isolates: A longitudinal analysis from 1994 to 2016

Tateda

Ohno

Ishii

et al. 2019

Journal of Infection and Chemotherapy

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“…Moreover, two ESBL-and the carbapenemase-producing isolates carried intI1, a gene encoding an element known to play a crucial role in the recruitment, spread and expression of resistance genes [66]. Numerous earlier studies have shown an association of both bla CTX-M and bla NDM with the variable region of class 1 integrons in E. coli isolates of human and animal origin [67][68][69][70]. Among wild animals, intl1 presence is related to their close contact with humans, farm animals and pets [71].…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Characteristics of ESBL- and Carbapenemase- Producing Escherichia coli from Wild and Feral Birds in Greece

et al. 2022

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Wild and feral birds are known to be involved in the maintenance and dissemination of clinically-important antimicrobial-resistant pathogens, such as extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae. The aim of our study was to evaluate the presence of ESBL- and carbapenemase-producing Escherichia coli among wild and feral birds from Greece and to describe their antimicrobial resistance characteristics. In this context, fecal samples of 362 birds were collected and cultured. Subsequently, the antimicrobial resistance pheno- and geno-type of all the obtained E. coli isolates were determined. A total of 12 multidrug-resistant (MDR), ESBL-producing E. coli were recovered from eight different wild bird species. Eleven of these isolates carried a blaCTX-M-1 group gene alone or in combination with blaTEM and one carried only blaTEM. AmpC, fluoroquinolone, trimethoprim/sulfamethoxazole, aminoglycoside and macrolide resistance genes were also detected. Additionally, one carbapenemase-producing E. coli was identified, harboring blaNDM along with a combination of additional resistance genes. This report describes the occurrence of ESBL- and carbapenemase-producing E. coli among wild avian species in Greece, emphasizing the importance of incorporating wild birds in the assessment of AMR circulation in non-clinical settings.

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“…Similarly, 91 and 53% of clinical isolates of Iran and Sweden were reported to produce ESBL with quinolone resistance, respectively (Shams et al . 2015; Kantele et al . 2017).…”

Section: Discussionmentioning

confidence: 99%

The use of eugenol in combination with cefotaxime and ciprofloxacin to combat ESBL‐producing quinolone‐resistant pathogenicEnterobacteriaceae

Dhara

Tripathi

2020

J Appl Microbiol

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Aim Emergence of extended‐spectrum beta‐lactamase (ESBL) producing with quinolone‐resistant (QR) pathogenic Enterobacteriaceae augmented the need to establish therapeutic options against them. Present study aimed towards determination of synergistic combination of eugenol (EG) with cefotaxime (CTX) and ciprofloxacin (CIP) to combat against this resistance and potentiation of antibacterial drugs by EG against these bacteria. Methods and Results Synergistic interaction between EG and CTX/CIP (FICI: 0·08–0·5) were observed among ESBL‐QR bacteria using checkerboard assay. Approximately, 2‐ to 1024‐fold minimum inhibitory concentration value reduction and 17‐ to 165 030‐fold dose reduction index strongly suggested synergistic interaction between EG and antibiotics. Cell viability assay showed reduction in log10 CFU per ml from 16·6 to 3·1 at synergistic concentration. Scanning electron microscopy further proved disruptive effect of EG on cell architecture. Eugenol and/or its combination also altered genes’ expressions that imparted antibiotic resistance by ~1·6 to ~1226 folds. Conclusions Reduced doses of antibiotics, bacterial morphological alterations, efflux pump down regulation, porin over expression and beta‐lactamase gene inhibition of ESBL‐QR bacteria by EG alone or in combination with CTX/CIP might have reversed antibiotic resistance profile of ESBL‐QR bacteria. Significance and Impact of the Study This study provided a molecular insight into action of EG and/with CTX and CIP, which might have potentiated antibiotic’s activity against ESBL‐QR bacteria.

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Carriage of Class 1 and 2 Integrons in Quinolone, Extended-Spectrum-β-Lactamase-Producing and Multi Drug Resistant E.coli and K.pneumoniae: High Burden of Antibiotic Resistance

Cited by 14 publications

References 40 publications

Investigation of the susceptibility trends in Japan to fluoroquinolones and other antimicrobial agents in a nationwide collection of clinical isolates: A longitudinal analysis from 1994 to 2016

Investigation of the susceptibility trends in Japan to fluoroquinolones and other antimicrobial agents in a nationwide collection of clinical isolates: A longitudinal analysis from 1994 to 2016

Occurrence and Characteristics of ESBL- and Carbapenemase- Producing Escherichia coli from Wild and Feral Birds in Greece

The use of eugenol in combination with cefotaxime and ciprofloxacin to combat ESBL‐producing quinolone‐resistant pathogenicEnterobacteriaceae

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