Carotenoids in Man. Iii. The Microscopic Pattern of Fluorescence in Atheromas, and Its Relation to Their Growth1

Blankenhorn, David H.; Braunstein, Herbert

doi:10.1172/jci103594

Cited by 41 publications

(14 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are similar to those of Kaminow (24), Anderson (31, 33), Wan (32), and van Gemert (25). Our identification of the yellow atheroma chromophores as carotenoids supports the work of Blankenhorn (38)(39)(40)(41)(42), who demonstrated carotenoids within atheromas more than 20 yr ago and showed that the amount of carotenoid in atheromas increases with the severity of the lesion. They are ubiquitous in nature, giving the red and yellow coloring of many vegetables (tomatoes, carrots, squash), animal products (egg yolk, butter, chicken fat), and exotic animals (parrots, canaries) (43).…”

Section: Methodssupporting

confidence: 89%

Preferential light absorption in atheromas in vitro. Implications for laser angioplasty.

Prince¹,

Deutsch²,

Mathews-Roth³

et al. 1986

J. Clin. Invest.

131

View full text Add to dashboard Cite

Laser angioplasty, the in situ ablation of arterial obstructions with laser radiation, has been demonstrated in animal models and early clinical trials. A problem with this technique, however, is the possibility of thermal damage to adjacent or underlying normal tissues that also absorb the radiation. Using a spectrophotometer with an integrating sphere and a specially constructed tunable-dye laser-based spectrophotometer, we evaluated the transmittance and remittance of human cadaveric atheromas and adjacent normal aorta from 250 to 1,300 unm to identify wavebands where there is preferential light absorption by atheromas. Data were analyzed by both the Kubelka-Munk formalism and a Beer's law model. Both methods indicate that atheromas absorb more than normal aorta between 420 and 530 nm. At 470 nm the average Kubelka-Munk absorption coefficient of atheromas from 10 cadavers was 54±9 cm-1 compared with 26±6 cm-' for normal aortic specimens from seven cadavers. Yellow chromophores responsible for the atheroma absorbance were extractable with xylenes. Thin-layer chromatography and absorption spectra identified the extracted chromophores as predominantly consisting of a mix of carotenoids, which are known constituents of atheromatous lesions. Preferential absorption of blue light by carotenoids in atheromas may permit selective ablation of atheromatous obstructions with appropriate pulses of laser radiation.

show abstract

Section: Methodssupporting

confidence: 89%

Preferential light absorption in atheromas in vitro. Implications for laser angioplasty.

Prince¹,

Deutsch²,

Mathews-Roth³

et al. 1986

J. Clin. Invest.

131

View full text Add to dashboard Cite

show abstract

“…24 Carotenoids have been used by others to detect atherosclerotic plaque. 30,31 In the present study, carotenoids were found to be present in greater amounts in plaques than in nonatherosclerotic tissue. For noncalcified plaques, the presence of carotenoids correlated with TC NCR (rϭ.85), and a diagnostic algorithm could be made based on the level of carotenoids and CS.…”

Section: Discussionsupporting

confidence: 48%

Histopathology of Human Coronary Atherosclerosis by Quantifying Its Chemical Composition With Raman Spectroscopy

et al. 1998

View full text Add to dashboard Cite

Background-Lesion composition, rather than size or volume, determines whether an atherosclerotic plaque will progress, regress, or rupture, but current techniques cannot provide precise quantitative information about lesion composition. We have developed a technique to assess the pathological state of human coronary artery samples by quantifying their chemical composition with near-infrared Raman spectroscopy. Methods and Results-Coronary artery samples (nϭ165) obtained from explanted recipient hearts were illuminated with 830-nm infrared light. Raman spectra were collected from the tissue and processed to quantify the relative weights of cholesterol, cholesterol esters, triglycerides and phospholipids, and calcium salts in the examined artery location. The artery locations were then classified by a pathologist and grouped as either nonatherosclerotic tissue, noncalcified plaque, or calcified plaque. Nonatherosclerotic tissue, which included normal artery and intimal fibroplasia, contained an average of Ϸ4Ϯ3% cholesterol, whereas noncalcified plaques had Ϸ26Ϯ10% and calcified plaques Ϸ19Ϯ10% cholesterol in the noncalcified regions. The average relative weight of calcium salts was 1Ϯ2% in noncalcified plaques and 41Ϯ21% in calcified plaques. To make this quantitative chemical information clinically useful, we developed a diagnostic algorithm, based on a first set of 97 samples, that demonstrated a strong correlation of the relative weights of cholesterol and calcium salts with histological diagnoses of the same locations. This algorithm was then prospectively tested on a second set of 68 samples. The algorithm correctly classified 64 of these new samples, thus demonstrating the accuracy and robustness of the method. Conclusions-The pathological state of a given human coronary artery may be assessed by quantifying its chemical composition, which can be done rapidly with Raman spectroscopic techniques. When Raman spectra are obtained clinically via optical fibers, Raman spectroscopy may be useful in monitoring the progression and regression of atherosclerosis, predicting plaque rupture, and selecting proper therapeutic intervention. (Circulation. 1998;97:878-885.)

show abstract

“…By using a combination of the different measurement procedures, it may be possible to better distinguish between the contributions to tissue fluorescence from various tissue chromophores. Fluorescence microscopy investigations as described by Blankenborn and Braunstein (1958) and chemical extraction used by e.g. Banga and Bihari-Varga (1974) are other methods useful in defining the chromophores responsible for tissue fluorescence.…”

Section: Tissue Fluorescence Characteristicsmentioning

confidence: 99%

Fluorescence Imaging and Point Measurements of Tissue: Applications to the Demarcation of Malignant Tumors and Atherosclerotic Lesions From Normal Tissue

Andersson‐Engels

Johansson

Svanberg

et al. 1991

Photochem & Photobiology

175

View full text Add to dashboard Cite

Abstract-The possibilities of using laser-induced fluorescence for tissue diagnostics are discussed. The tissue types investigated arc malignant tumors and atherosclerotic lesions. Studies with natural autofluorescence as well as with fluorescent tumor markers are included in this paper. Fluorescence emission and decay data are presented for some tissue chromophores contributing to tissue autofluorescence. Optical spectroscopic characteristics Of fluorescent malignant tumor markers are analyzed and instrumental designs for clinical applications are discussed. Images recorded with a multicolor fluorescence imaging system developed in Lund are presented.

show abstract

Carotenoids in Man. Iii. The Microscopic Pattern of Fluorescence in Atheromas, and Its Relation to Their Growth1

Cited by 41 publications

References 6 publications

Preferential light absorption in atheromas in vitro. Implications for laser angioplasty.

Preferential light absorption in atheromas in vitro. Implications for laser angioplasty.

Histopathology of Human Coronary Atherosclerosis by Quantifying Its Chemical Composition With Raman Spectroscopy

Fluorescence Imaging and Point Measurements of Tissue: Applications to the Demarcation of Malignant Tumors and Atherosclerotic Lesions From Normal Tissue

Contact Info

Product

Resources

About