Carotenoids in female and male reproduction

Pasquariello, Rolando; Anipchenko, Polina; Pennarossa, G.; Crociati, Martina; Zerani, Massimo; Brevini, Tiziana AL.; Gandolfi, F.; Maranesi, Margherita

doi:10.1016/j.phytochem.2022.113459

Cited by 8 publications

(6 citation statements)

References 172 publications

(210 reference statements)

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“…The major impact of oxidative stress on fertility has also been highlighted by the fact that many intervention strategies to face infertility are just addressed to reduce oxidative stress—for example, using antioxidant molecules ( 23 – 31 ). In this regard, our recent research focuses on the cellular and differentiative mechanisms that regulate spermatogenesis, which was perturbed using experimental models of oxidative stress ( 32 , 33 ), to expand the knowledge of the molecular pathways involved in spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

Type 1 diabetes impairs the activity of rat testicular somatic and germ cells through NRF2/NLRP3 pathway-mediated oxidative stress

Venditti,

Romano,

Boccella

et al. 2024

Front. Endocrinol.

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BackgroundIt is well known that metabolic disorders, including type 1 diabetes (T1D), are often associated with reduced male fertility, mainly increasing oxidative stress and impairing the hypothalamus–pituitary–testis (HPT) axis, with consequently altered spermatogenesis and reduced sperm parameters. Herein, using a rat model of T1D obtained by treatment with streptozotocin (STZ), we analyzed several parameters of testicular activity.MethodsA total of 10 adult male Wistar rats were divided into two groups of five: control and T1D, obtained with a single intraperitoneal injection of STZ. After 3 months, the rats were anesthetized and sacrificed; one testis was stored at -80°C for biochemical analysis, and the other was fixed for histological and immunofluorescence analysis.ResultsThe data confirmed that T1D induced oxidative stress and, consequently, alterations in both testicular somatic and germ cells. This aspect was highlighted by enhanced apoptosis, altered steroidogenesis and Leydig cell maturity, and impaired spermatogenesis. In addition, the blood–testis barrier integrity was compromised, as shown by the reduced levels of structural proteins (N-cadherin, ZO-1, occludin, connexin 43, and VANGL2) and the phosphorylation status of regulative kinases (Src and FAK). Mechanistically, the dysregulation of the SIRT1/NRF2/MAPKs signaling pathways was proven, particularly the reduced nuclear translocation of NRF2, affecting its ability to induce the transcription of genes encoding for antioxidant enzymes. Finally, the stimulation of testicular inflammation and pyroptosis was also confirmed, as highlighted by the increased levels of some markers, such as NF-κB and NLRP3.ConclusionThe combined data allowed us to confirm that T1D has detrimental effects on rat testicular activity. Moreover, a better comprehension of the molecular mechanisms underlying the association between metabolic disorders and male fertility could help to identify novel targets to prevent and treat fertility disorders related to T1D.

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Section: Introductionmentioning

confidence: 99%

Type 1 diabetes impairs the activity of rat testicular somatic and germ cells through NRF2/NLRP3 pathway-mediated oxidative stress

Venditti,

Romano,

Boccella

et al. 2024

Front. Endocrinol.

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Section: Introductionmentioning

confidence: 99%

“…Carotenoids are an important source of vitamin A[4] and play an important role in the treatment of vitamin A de ciency (VAD). In addition, carotenoids have various physiological functions such as antioxidant, eye and skin protection, and prevention of cardiovascular diseases [4][5][6][7][8].Genetic diversity is a fundamental part of biodiversity and is the basis for ecological and species diversity. It usually refers to the sum of genetic variation among different groups of species or among individuals within a group[9, 10].…”

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confidence: 99%

Genetic diversity and population structure of sweet potato landraces based on phenotypic traits, carotenoid content, and SSR molecular markers

Chen

Tang

Zhang

et al. 2023

Preprint

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Germplasm resources are an important basis for genetic breeding, and the study of genetic diversity is beneficial to the discovery and improvement of germplasm resources. In this study, 132 local germplasm of sweet potato were used as test materials, and 20 phenotypic traits were analyzed for genetic diversity using distribution frequency, coefficient of variation, and Shannon's diversity index; carotenoid contents in sweet potato tubers were measured by HPLC, and their genetic diversity was analyzed using the coefficient of variation, mean, and standard deviation. Meanwhile, the genetic diversity, as well as population structure, were analyzed based on 10 pairs of SSR molecular markers using Nei's genetic diversity index and Shannon's diversity index. The results showed that the 132 sweet potato landraces had high genetic diversity based on phenotypic traits, SSR molecular markers, and carotenoid content, respectively; based on population structure analysis, the 132 sweet potato landraces could be divided into five subgroups. Analysis of molecular variation (AMOVA) showed that 84% of the variation existed within populations and 16% between populations. This study provides a basis for the selection of parental material for the genetic breeding improvement of sweet potato and for the mining of superior genes.

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“…Astaxanthin, in its role as a natural antioxidant, could potentially lessen the harmful effects of oxidative stress on reproductive cells and tissues (Li et al 2022). Additionally, this carotenoid may in uence hormone production and signaling pathways related to reproduction, ultimately in uencing reproductive success and outcomes (Pasquariello et al 2022). The precise mechanisms underlying astaxanthin's effects on reproductive processes are still being explored, but the existing evidence suggests its potential as a valuable component in the modulation of reproductive functions.…”

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confidence: 99%

“…Carotenoids, in a role similar to that in mammals, exhibit a steroidogenic function and serve as regulators of folliculogenesis and oogenesis in sh. And the presence of retinol and retinoic acid, which are crucial for the development of mature sperm, further highlights the signi cance of carotenoids in reproductive functions(Pasquariello et al 2022). Furthermore, astaxanthin serves as an essential source of retinol and 3,4-didehydroretinol, which are precursors of vitamin A and have crucial roles during the development ofvertebrate embryos (Moren et al 2002; Blomhoff and Blomhoff 2006; Duester 2008; Kin Ting Kam et al 2012).…”

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confidence: 99%

Impacts of astaxanthin-enriched Paracoccus carotinifaciens on growth, immune responses, and reproduction performance of broodstock Nile tilapia during winter season

Panase,

Vongkampang,

Wangkahart

et al. 2024

Fish Physiol Biochem

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The growth, immune response, and reproductive performance of broodstock of Nile tilapia (Oreochromis niloticus) under winter stress conditions were investigated the effects of supplementary diets with astaxanthin-enriched bacterium, Paracoccus carotinifaciens. Throughout an eight-week period in the winter season, male and female tilapia were fed with diets containing different levels of P. carotinifaciens dietary supplementation: 0 g/kg (T1; control), 5 g/kg (T2), 10 g/kg (T3), and 20 g/kg (T4). Subsequently, a four-week mating system was implemented during the winter stress period. The results revealed that there were no signi cant differences observed in growth, hematological indices, and blood chemical pro les among all treatment groups for both male and female tilapia. However, a signi cant increase in cholesterol content was noted in both male and female tilapia fed with the T4 diet (p<0.05). The total carotenoid content in the muscle was evaluated, and signi cantly higher values were found in both male and female tilapia that fed T4 supplementation (p<0.05). Moreover, immunological parameters such as myeloperoxidase and antioxidant parameters in the liver including superoxide dismutase activity and catalase enzyme activity showed signi cant increases in tilapia fed with the T4 diet. The impact of P. carotinifacienssupplementation on broodstock tilapia indicated a signi cant increase in spermatozoa concentration in males and increased egg production in females after consumption of the T4 diet (p<0.05). Thus, this study highlighted that the presence of astaxanthin-enriched bacterium P. carotinifaciensin the diet of broodstock Nile tilapia can lead to the accumulation of carotenoids in their muscle tissue, improvement in antioxidant status, enhancement of immune function, and potential enhancement of reproductive capabilities, even under overwintering conditions.

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Carotenoids in female and male reproduction

Cited by 8 publications

References 172 publications

Type 1 diabetes impairs the activity of rat testicular somatic and germ cells through NRF2/NLRP3 pathway-mediated oxidative stress

Type 1 diabetes impairs the activity of rat testicular somatic and germ cells through NRF2/NLRP3 pathway-mediated oxidative stress

Genetic diversity and population structure of sweet potato landraces based on phenotypic traits, carotenoid content, and SSR molecular markers

Impacts of astaxanthin-enriched Paracoccus carotinifaciens on growth, immune responses, and reproduction performance of broodstock Nile tilapia during winter season

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