Carotenoids in a Corynebacterineae,<i>Gordonia terrae</i>AIST-1: Carotenoid Glucosyl Mycoloyl Esters

Takaichi, Shinichi; Maoka, Takashi; Akimoto, Naoshige; Carmona, Marvelisa L.; Yamaoka, Yukiho

doi:10.1271/bbb.80299

Cited by 19 publications

(9 citation statements)

References 22 publications

(31 reference statements)

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“…Strains of A. globiformis have shown the ability to produce a compound with high antioxidant capacity [26]. The actinomycete G. lacunae exhibited antibiotic activity [25], whereas G. terrae was reported as a carotenoids producer [27]. Interestingly, none of the genera identified in this study had previously been reported in agave sap or pulque (fermented agave sap).…”

Section: Metabolomicsmentioning

confidence: 65%

Mass Spectrometry-Based Metabolomics of Agave Sap (Agave salmiana) after Its Inoculation with Microorganisms Isolated from Agave Sap Concentrate Selected to Enhance Anticancer Activity

et al. 2017

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Abstract:Saponins have been correlated with the reduction of cancer cell growth and the apoptotic effect of agave sap concentrate. Empirical observations of this artisanal Mexican food have shown that fermentation occurs after agave sap is concentrated, but little is known about the microorganisms that survive after cooking, or their effects on saponins and other metabolites. The aim of this study was to evaluate the changes in metabolites found in agave (A. salmiana) sap after its fermentation with microorganisms isolated from agave sap concentrate, and demonstrate its potential use to enhance anticancer activity. Microorganisms were isolated by dilution plating and identified by 16S rRNA analysis. Isolates were used to ferment agave sap, and their corresponding butanolic extracts were compared with those that enhanced the cytotoxic activity on colon (Caco-2) and liver (Hep-G2) cancer cells. Metabolite changes were investigated by mass spectrometry-based metabolomics. Among 69 isolated microorganisms, the actinomycetes Arthrobacter globiformis and Gordonia sp. were used to analyze the metabolites, along with bioactivity changes. From the 939 ions that were mainly responsible for variation among fermented samples at 48 h, 96 h, and 192 h, four were correlated to anticancer activity. It was shown that magueyoside B, a kammogenin glycoside, was found at higher intensities in the samples fermented with Gordonia sp. that reduced Hep-G2 viability better than controls. These findings showed that microorganisms from agave sap concentrate change agave sap metabolites such as saponins. Butanolic extracts obtained after agave sap fermentation with Arthrobacter globiformis or Gordonia sp. increased the cancer cell growth inhibitory effect on colon or liver cancer cells, respectively.

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Section: Metabolomicsmentioning

confidence: 65%

Mass Spectrometry-Based Metabolomics of Agave Sap (Agave salmiana) after Its Inoculation with Microorganisms Isolated from Agave Sap Concentrate Selected to Enhance Anticancer Activity

et al. 2017

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“…An actinomycete genera Gordonia is closely related to genus Rhodococcus having same ancestral origin (Stackebrandt et al, 1997). Thus, few Gordonia species that are known to produce ϒ-carotene and its derivatives (Takaichi et al, 2008, Sowani et al, 2017 were also included for phylogeny analysis. Three proteins identified to be crucial for the carotenoid production in R. kroppenstedtii; lycopene β-cyclase (crtY), phytoene dehydrogenase related protein (crtO type) and hydroxyneurosporene synthase (crtC) were considered in this study.…”

Section: In Silico Studiesmentioning

confidence: 99%

Isolation and characterization of a rare polar carotenoid 1’-OH-4-keto-ϒ-carotene from an indigenously isolated Rhodococcus kroppenstedtii MH715196

Lilwani

Dokhale

JR³

et al. 2022

FSAB

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The catabolic diversity and biocatalytic potential in members of genus Rhodococcus makes it an ideal industrial workhouse for metabolite production. Despite their applicability, Rhodococci are least explored for carotenoids, located in their cell membrane. The goal of the present study was to identify the carotenoids of Rhodococcus kroppenstedtii MH715196. UV-Vis spectral data and molecular mass estimates showed that the principal carotenoid extracted from R. kroppenstedtii MH715196 was 1’-OH-4-keto-ϒ-carotene. Biosynthetic route of 1’-OH-4-keto-ϒ-carotene in R. kroppenstedtii MH715196 was postulated on the basis of molecular mass estimates in co-relation with putative carotenoid biosynthetic genes identified in the genome of the reference strains of R. kroppenstedtii. Three key enzymes, were then considered for phylogenetic analysis to establish the phylogenetic relationship across the Rhodococcus genus leading the way for carotenoid identification in other Rhodococcus species. The present study would be the first report on identification of a rare polar carotenoid from R. kroppenstedtii MH715196 which could be potentially explored as a food colorant in hydrophilic food matrices like jams, jellies, beverages etc.

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“…Myxol biosynthesis has been less explored than other pathways related to carotenogenesis, and most of the results reported came from studies on cyanobacteria [49][50][51]. γ-carotene glucosides are poorly described in the literature, and they are usually described as "rare" carotenoids [34,52].…”

Section: Reconstruction Of a Metabolic Map For Global Carotenogenesismentioning

confidence: 99%

Deciphering Pathways for Carotenogenesis in Haloarchaea

et al. 2020

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Bacterioruberin and its derivatives have been described as the major carotenoids produced by haloarchaea (halophilic microbes belonging to the Archaea domain). Recently, different works have revealed that some haloarchaea synthetize other carotenoids at very low concentrations, like lycopene, lycopersene, cis- and trans-phytoene, cis- and trans-phytofluene, neo-β-carotene, and neo-α-carotene. However, there is still controversy about the nature of the pathways for carotenogenesis in haloarchaea. During the last decade, the number of haloarchaeal genomes fully sequenced and assembled has increased significantly. Although some of these genomes are not fully annotated, and many others are drafts, this information provides a new approach to exploring the capability of haloarchaea to produce carotenoids. This work conducts a deeply bioinformatic analysis to establish a hypothetical metabolic map connecting all the potential pathways involved in carotenogenesis in haloarchaea. Special interest has been focused on the synthesis of bacterioruberin in members of the Haloferax genus. The main finding is that in almost all the genus analyzed, a functioning alternative mevalonic acid (MVA) pathway provides isopentenyl pyrophosphate (IPP) in haloarchaea. Then, the main branch to synthesized carotenoids proceeds up to lycopene from which β-carotene or bacterioruberin (and its precursors: monoanhydrobacterioriberin, bisanhydrobacterioruberin, dihydrobisanhydrobacteriuberin, isopentenyldehydrorhodopsin, and dihydroisopenthenyldehydrorhodopsin) can be made.

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Carotenoids in a Corynebacterineae,Gordonia terraeAIST-1: Carotenoid Glucosyl Mycoloyl Esters

Cited by 19 publications

References 22 publications

Mass Spectrometry-Based Metabolomics of Agave Sap (Agave salmiana) after Its Inoculation with Microorganisms Isolated from Agave Sap Concentrate Selected to Enhance Anticancer Activity

Mass Spectrometry-Based Metabolomics of Agave Sap (Agave salmiana) after Its Inoculation with Microorganisms Isolated from Agave Sap Concentrate Selected to Enhance Anticancer Activity

Isolation and characterization of a rare polar carotenoid 1’-OH-4-keto-ϒ-carotene from an indigenously isolated Rhodococcus kroppenstedtii MH715196

Deciphering Pathways for Carotenogenesis in Haloarchaea

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