Carotenoids improve bacterial tolerance towards biobutanol through membrane stabilization

Chia, Geraldine W. N.; Seviour, Thomas; Kjelleberg, Staffan; Hinks, Jamie

doi:10.1039/d0en00983k

Cited by 8 publications

(6 citation statements)

References 53 publications

(101 reference statements)

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“…The disruption of membrane integrity and the increase of membrane fluidity were also observed and assumed to be crucial factors in determining butanol toxicity. Subsequent experimental methods 223 confirmed that carotenoids could reduce butanol penetration and membrane fluidization effect by fortifying cell membranes and thus improve butanol tolerance in E. coli.…”

Section: Effects Of Small Organic Molecules On Membranesmentioning

confidence: 96%

Application of computational approaches in biomembranes: From structure to function

Guo

Bao

et al. 2023

WIREs Comput Mol Sci

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Biological membranes (biomembranes) are one of the most complicated structures that allow life to exist. Investigating their structure, dynamics, and function is crucial for advancing our knowledge of cellular mechanisms and developing novel therapeutic strategies. However, experimental investigation of many biomembrane phenomena is challenging due to their compositional and structural complexity, as well as the inherently multi‐scalar features. Computational approaches, particularly molecular dynamics (MD) simulations, have emerged as powerful tools for addressing the atomic details of biomembrane systems, driving breakthroughs in our understanding of biomembranes and their roles in cellular function. This review presents an overview of the latest advancements in related computational approaches, from force fields and model construction to MD simulations and trajectory analysis. We also discussed current hot research topics and challenges. Finally, we outline future directions, emphasizing the integration of force field development, enhanced sampling techniques, and data‐driven approaches to accelerate the growth of this field in the years to come. We aim to equip readers with an understanding of the promise and limitations of emerging computational technologies in biomembrane systems and offer valuable recommendations for future research endeavors.This article is categorized under: Structure and Mechanism > Computational Biochemistry and Biophysics Molecular and Statistical Mechanics > Molecular Dynamics and Monte‐Carlo Methods

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Section: Effects Of Small Organic Molecules On Membranesmentioning

confidence: 96%

Application of computational approaches in biomembranes: From structure to function

Guo

Bao

et al. 2023

WIREs Comput Mol Sci

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show abstract

“…They present significant biological properties, for example, acting as antioxidants and protecting cells and tissues from the harmful effects of free radicals and singlet oxygen [ 55 ]. In addition, these compounds were reported to engage in photoprotection, and membrane stabilization [ 56 , 57 , 58 ]. Certain studies report that carotenoids possibly lowered the phase transition temperature of synthetic lipids and worked as regulators for membrane fluidity since they showed an important role in the causative function in the modulation of membrane parameters [ 59 , 60 ].…”

Section: Significance Of Arthrobacter Spp In the D...mentioning

confidence: 99%

Involvement of Versatile Bacteria Belonging to the Genus Arthrobacter in Milk and Dairy Products

Sutthiwong

Lekavat

Dufossé

2023

Foods

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Milk is naturally a rich source of many essential nutrients; therefore, it is quite a suitable medium for bacterial growth and serves as a reservoir for bacterial contamination. The genus Arthrobacter is a food-related bacterial group commonly present as a contaminant in milk and dairy products as primary and secondary microflora. Arthrobacter bacteria frequently demonstrate the nutritional versatility to degrade different compounds even in extreme environments. As a result of their metabolic diversity, Arthrobacter species have long been of interest to scientists for application in various industry and biotechnology sectors. In the dairy industry, strains from the Arthrobacter genus are part of the microflora of raw milk known as an indicator of hygiene quality. Although they cause spoilage, they are also regarded as important strains responsible for producing fermented milk products, especially cheeses. Several Arthrobacter spp. have reported their significance in the development of cheese color and flavor. Furthermore, based on the data obtained from previous studies about its thermostability, and thermoacidophilic and thermoresistant properties, the genus Arthrobacter promisingly provides advantages for use as a potential producer of β-galactosidases to fulfill commercial requirements as its enzymes allow dairy products to be treated under mild conditions. In light of these beneficial aspects derived from Arthrobacter spp. including pigmentation, flavor formation, and enzyme production, this bacterial genus is potentially important for the dairy industry.

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“…The authors suggest using carotene-rich waste (e.g. tomato pomace) as a cheap carbon and carotene source, making this approach even more interesting for the sustainable production of chemicals [ 58 ].…”

Section: (Semi-) Rational Approachesmentioning

confidence: 99%

Strategies to increase tolerance and robustness of industrial microorganisms

Mohedano

Konzock

Chen

2022

Synthetic and Systems Biotechnology

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The development of a cost-competitive bioprocess requires that the cell factory converts the feedstock into the product of interest at high rates and yields. However, microbial cell factories are exposed to a variety of different stresses during the fermentation process. These stresses can be derived from feedstocks, metabolism, or industrial production processes, limiting production capacity and diminishing competitiveness. Improving stress tolerance and robustness allows for more efficient production and ultimately makes a process more economically viable. This review summarises general trends and updates the most recent developments in technologies to improve the stress tolerance of microorganisms. We first look at evolutionary, systems biology and computational methods as examples of non-rational approaches. Then we review the (semi-)rational approaches of membrane and transcription factor engineering for improving tolerance phenotypes. We further discuss challenges and perspectives associated with these different approaches.

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Carotenoids improve bacterial tolerance towards biobutanol through membrane stabilization

Abstract: The nano-aggregation of carotenoids in microbial membranes increases membrane stability upon butanol exposure by reducing the fluidization effect and membrane permeability of butanol.

Cited by 8 publications

References 53 publications

Application of computational approaches in biomembranes: From structure to function

Application of computational approaches in biomembranes: From structure to function

Involvement of Versatile Bacteria Belonging to the Genus Arthrobacter in Milk and Dairy Products

Strategies to increase tolerance and robustness of industrial microorganisms

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