Carotenoids

Ninet, L.; Renaut, J.-J.

doi:10.1016/b978-0-12-551501-6.50022-x

Cited by 20 publications

(8 citation statements)

References 18 publications

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“…The industrial procedures described previously (10,29) were aimed at the production of ␤-carotene, an attractive pigment with provitamin A activity and various positive health effects. Lycopene, the red tomato pigment, was not in much demand, but the situation has changed recently.…”

Section: Discussionmentioning

confidence: 99%

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Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Mehta

Obraztsova

Cerdá‐Olmedo

2003

Appl Environ Microbiol

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The industrial production of ␤-carotene with the zygomycete Blakeslea trispora involves the joint cultivation of mycelia of opposite sex in the presence of ␤-ionone and other chemical activators. We have obtained improved strains by mutation and heterokaryosis. We chose wild strains on the basis of their growth and carotene content in single and mated cultures. Following exposure of their spores to N-methyl-N-nitro-Nnitrosoguanidine, we obtained high-carotene mutants, which were more productive than their parents but similar to them in having ␤-carotene as the main product. Further increases in carotene content were obtained after a new round of mutagenesis in one of the mutants. The production was shifted to lycopene in cultures incubated in the presence of nicotine and in lycopene-rich mutants derived from the wild strains. The highest production levels were achieved in intersexual heterokaryons, which contained mutant nuclei of opposite sex. These contained up to 39 mg of ␤-carotene or 15 mg of lycopene per g (dry mass) under standard laboratory conditions in which the original wild strains contained about 0.3 mg of ␤-carotene per g (dry mass). ␤-Ionone did not increase the carotene content of these strains. Not all wild strains lent themselves to these improvements, either because they produced few mutants or because they did not increase their carotene production in mated cultures.

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Section: Discussionmentioning

confidence: 99%

“…Blakeslea trispora, a zygomycete mold, is an industrial source of ␤-carotene following the optimization of liquid fermentation processes (10,29). The organism is a saprophyte that performs its vegetative cycle of spores, filamentous mycelia, fruiting bodies, and again spores on chemically defined media in the laboratory.…”

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confidence: 99%

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Mehta

Obraztsova

Cerdá‐Olmedo

2003

Appl Environ Microbiol

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“…Finally, strain P47 cells contained 0.8 mg/g of cells of carotenoids, the highest among the photosynthetic bacteria. NINET and RENAUT (33) reported that the biological function of carotenoid in animal feed is to intensify the Table 2. Growth characteristics, vitamin B12 and photopigment accumulations of strain P47 in contrast to Rb.…”

Section: And Discussionmentioning

confidence: 99%

Selection of Rhodobacter sphaeroides P47 as a useful source of single cell protein.

Noparatnaraporn

Nagai

1986

J. Gen. Appl. Microbiol.

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Rhodobacter sphaeroides P47 was selected as a potent microorganism for SCP production. The selection criteria were: high growth rate, high growth yield from consumed sugar, ability to consume various sugars, high protein content with a balanced amino acid composition, and acceptable contents of vitamin B12 and carotenoids. When the cells grew aerobically on minimal medium containing glucose, fructose and xylose as sole carbon sources, they grew at the relatively high specific growth rate of 0.12-0.161/hr. Also, the growth yield from sugar, 0.52-0.55 g of cells/g of sugar, was high compared with Rb. sphaeroides S as a standard. In the sugar uptake test, strain P47 not only preferably consumed glucose, fructose, xylose and mannose, but also consumed maltose, sucrose, cellobiose and treharose. Strain S consumed only glucose, fructose, xylose and mannose. Strain P47 cells cultured on pineapple waste medium had a relatively high protein content (66.6%) with balanced amino acid composition. The intracellular contents of vitamins B12 and E and carotenoids were 7.9, 21, and 80 mg/ 100 g of dry cells, respectively. f hese values were considerably higher than those of other photosynthetic bacteria.To be suitable for single cell protein (SCP) production (1), microorganisms should: grow fast with efficient growth yield from carbon substrates; attain a high cell density with diverse substrate utilization; and contain balanced nutrients. Regarding potential microbial resources for SCP, several researchers (2-6) have attempted to cultivate photosynthetic bacteria on agroindustrial waste materials, eg. soybean whey, polluted water from soybean curd manufacture, swine excreta (2), wheat bran (3), soybean waste (5) and cassava starch (6). This should be feasible since the bacteria can grow on various carbon substrates under either phototrophic or heterotrophic conditions and their protein content is relatively high

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“…This explains the increasing interest in production of microbial carotenoids as alternative for synthetic food colourants [1]. Several algae (Dunaliella, Dictyococcus and Haematococcus), bacteria (many species of eubacteria in addition to halobacteria in archaebacteria), some filamentous fungi (belong to lower fungi and Ascomycetes), yeasts (Cryptococcus, Phaffia, Rhodosporidium, Rhodotorula, Sporidiobolus, and Sporobolomyces) are reported to produce carotenoids [1,[6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Screening of Carotenoid-Producing Strains of <i>Rhodotorula glutinis</i>

El‐Banna¹,

El-Razek²,

El-Mahdy³

2012

FNS

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An attempt was made to isolate yeast strains from different sources (flowers, trees exudates, fruits and cheese) which have a fast growth rate and high content of carotenoids. Seventy cultures of pigmented yeast were isolated. Forty six yeast isolates were selected and identified by simplified identification method which showed that all isolates belong to Rhodotorula glutinis. These isolates were screened in two steps. Seventeen promising isolates of Rhodotorula glutinis were selected after the primary screening. Then 6 yeast isolates were chosen and passed through the second screening. The most promising isolate (isolated from pin cushion flower, Scabiosa atropurpurea) was selected according to its total carotenoids content expressed as torulene (μg·g -1 and μg·L -1 ). This isolate was reclassified using a number of morphological, biochemical, and physiological characteristics which revealed that the yeast isolate agree well with those of Rhodotorula glutinis var. glutinis. Dry biomass, amount and proportion of individual carotenoids and lipid content of the selected identified yeast were determined.

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Carotenoids

Cited by 20 publications

References 18 publications

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Selection of Rhodobacter sphaeroides P47 as a useful source of single cell protein.

Isolation, Identification and Screening of Carotenoid-Producing Strains of <i>Rhodotorula glutinis</i>

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Carotenoids

Cited by 20 publications

References 18 publications

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Selection of Rhodobacter sphaeroides P47 as a useful source of single cell protein.

Isolation, Identification and Screening of Carotenoid-Producing Strains of &lt;i&gt;Rhodotorula glutinis&lt;/i&gt;

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Isolation, Identification and Screening of Carotenoid-Producing Strains of <i>Rhodotorula glutinis</i>