Carotenoid profile of three microalgae/cyanobacteria species with peroxyl radical scavenger capacity

Patias, Luciana; Fernandes, A. S.; Petry, Fabiane C.; Mercadante, Adriana Zerlotti; Jacob‐Lopes, Eduardo; Zepka, Leila Queiroz

doi:10.1016/j.foodres.2017.06.069

Cited by 83 publications

(44 citation statements)

References 36 publications

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“…In the case of lutein, several commonly observed isomers with one cis double bond were also detected (Table 1). Both chlorophyll a and b were clearly identified, along with their known epimeric isomers (Asker et al 2018;Patias et al 2017;Rivera et al 2014). Similar to previous studies (Deli et al 2014), this strain did not produce any observable carotenoid esters.…”

Section: Figuresupporting

confidence: 88%

“…Therefore, HPLC experiments that track absorption in both regions can be used to reveal this group of compounds. Additionally, comparisons with a variety of previous studies (Borowitzka et al 2016;Patias et al 2017;Rivera et al 2014) are also a great tool to match the identities of each peak. Taken together, we could identify several molecular species and all of the data are shown in Fig.…”

Section: Figurementioning

confidence: 99%

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Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Janchot

Rauytanapanit

Honda

et al. 2019

J Eukaryotic Microbiology

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Microalgae are a diverse group of photosynthetic eukaryotic organisms that are widely distributed globally. They are prolific sources of highly valuable compounds with fascinating chemical structures. Due to their balanced nutritional compositions and health benefits, they are increasingly being used as functional food ingredients. Carotenoid‐based pigments and polyunsaturated fatty acids (PUFAs) are examples of high‐value nutrients that can be accumulated abundantly in microalgae. Here, the effects of potassium chloride‐induced stress on the productions of lipids and carotenoids in the green microalga of the Chlorococcaceae family were investigated. Under normal BG11 medium, this green microalga strain TISTR 9500 accumulated high levels of PUFA and primary carotenoid lutein. Stress tests revealed that KCl enhanced and modulated lipid and carotenoid accumulation levels. The liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis revealed that secondary carotenoids astaxanthin and canthaxanthin were robustly produced under KCl stress with the similar content of lutein. Further, this stress led to a significant increase in the total FA amount with the higher proportion of unsaturated FA than saturated FA. Thus, this green microalga could be an attractive and alternative natural biosource for canthaxanthin and astaxanthin, as well as for functional lipids.

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Section: Figuresupporting

confidence: 88%

Section: Figurementioning

confidence: 99%

Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Janchot

Rauytanapanit

Honda

et al. 2019

J Eukaryotic Microbiology

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“…Patias et al [39] have also reported a correlation between carotenoid profiles and the antioxidant potential of extracts obtained from different cyanobacteria of the genus Aphanothece, Chlorella and Scenedesmus, inferring that samples richer in highly unsaturated carotenoids are more effective as radical scavengers. Aside from this, we have noted that chlorophylls may also contribute to O 2…”

Section: •-mentioning

confidence: 94%

Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis

2020

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In this study, five cyanobacteria strains (Alkalinema aff. pantanalense LEGE15481, Cyanobium gracile LEGE12431, Nodosilinea (Leptolyngbya) antarctica LEGE13457, Cuspidothrix issatschenkoi LEGE03282 and Leptolyngbya-like sp. LEGE13412) from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE CC) of CIIMAR were explored for their biotechnological potential in the treatment of psoriasis. Different extracts were characterized for their pigment profile by HPLC-PDA. The antioxidant potential of the extracts was assessed against the superoxide anion radical (O2•-). Their anti-inflammatory and antiproliferative potential was assessed in vitro using the macrophages RAW 264.7 and the human keratinocytes HaCaT as cell-line models, respectively. Terrestrial and freshwater strains presented the highest carotenoid content (33193−63926 μg/g dry extract), with all-trans-β-carotene, zeaxanthin, echinenone and lutein derivatives being the most abundant carotenoids. Acetone was the most effective solvent for pigment extraction. The acetone extracts presented the lowest IC50 values (0.29−0.38 mg dry extract/mL) regarding O2•- scavenging, and revealed anti-inflammatory potential, with N. antarctica LEGE13457, A. pantanalense LEGE15481 and Leptolyngbya-like sp. LEGE13412 reducing the nitric oxide (NO) in RAW 264.7 cell culture medium in about 25% (p < 0.05). With the exception of A. pantanalense LEGE15481, all the extracts significantly reduced keratinocyte proliferation (p < 0.05), demonstrating a selective toxicity among the different cell lines. Overall, Leptolyngbya-like sp. LEGE13412 and N. antarctica LEGE13457 seem promising for further exploitation in the framework of psoriasis, due to their antioxidant, anti-inflammatory and antiproliferative potential.

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“…HPLC was performed using an Agilent 1100 HPLC system (Agilent Technologies, Palo Alto, CA, USA) equipped with a PDA detector and YMC carotenoid C 30 column (5 μm, 250 mm × 4.6 mm; Waters Corp., Milford, MA, USA). The mobile phase consisted of solvent A (92% methanol in 10 mM ammonium acetate) and solvent B (100% methyl tert-butyl ether) at varying ratios 55 . The flow rate and column temperature were maintained at 1 ml/min and 40 °C, respectively.…”

Section: Construction Of Plasmids Expressing Carotenoid Biosynthesis mentioning

confidence: 99%

Genomic Survey of Salt Acclimation-Related Genes in the Halophilic Cyanobacterium Euhalothece sp. Z-M001

Yang

Song

Cho

et al. 2020

Sci Rep

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, cheol-Ho pan 2 & Youn-il park 1* Like other halophilic cyanobacterial genomes, the de novo-assembled genome of Euhalothece sp. Z-M001 lacks genes encoding keto-carotenoid biosynthesis enzymes, despite the presence of genes encoding carotenoid-binding proteins (cBps). consistent with this, HpLc analysis of carotenoids identified β-carotene and zeaxanthin as the dominant carotenoids. CBPs coexpressed with the zeaxanthin biosynthesis gene increased the survival rates of Escherichia coli strains by preventing antibiotic-induced accumulation of reactive oxygen species (ROS). RNA-seq analysis of Euhalothece revealed that among various salt resistance-related genes, those encoding the na + transporting multiple resistance and pH adaptation (Mrp) systems, glycine betaine biosynthesis enzymes, exopolysaccharide metabolic enzymes, and CBPs were highly upregulated, suggesting their importance in hypersaline habitats. During the early phase of salt deprivation, the amounts of β-carotene and zeaxanthin showed a negative correlation with ROS content. Overall, we propose that in some halophilic cyanobacteria, β-carotene and zeaxanthin, rather than keto-carotenoids, serve as the major chromophores for CBPs, which in turn act as effective antioxidants. Cyanobacteria are photosynthetic prokaryotes that exhibit diverse protective mechanisms to cope with harsh environmental conditions. One of the protective mechanisms involves the use of carotenoids 1 and diverse carotenoid-binding proteins (CBPs) 2 , which play essential roles in protecting the photosynthetic apparatus from damage by both excess light energy and reactive oxygen species (ROS). Most cyanobacterial CBPs bind to β-carotene (β-Car) and its oxygenated derivatives, called xanthophylls (Xan), and are located in photosynthetic protein complexes such as photosystem I (PSI), PSII, and cytochrome b 6 f 3. By contrast, water soluble CBPs bind non-covalently to cyanobacterial keto-carotenoids, such as echinenone (Ech) and canthaxanthin (Can), and to glycosylated-carotenoids such as myxoxanthophyll (Myx) 4 ; CBPs then function as effective energy dissipaters by interacting with the light-harvesting antenna, phycobilisome (PBS) 5. CBPs also bind to zeaxanthin (Zea), albeit with low affinity 6. The orange carotenoid protein (OCP) and its two paralogs, helical carotenoid protein (HCP) and C-terminal domain homolog (CTDH), are the only known soluble CBPs 4. The OCP is composed of two globular domains (one each at the N-and C-terminal ends); a single carotenoid is embedded between the two domains via a hydrophobic interaction 7. Blue light absorption by a keto-carotenoid results in a conformational change in the OCP, from the orange inactive form (OCP°) to the red active form (OCP R). Binding of OCP R to PBS quenches PBS fluorescence, which is prevented by fluorescence recovery protein (FRP) 8. FRP prevents accumulation of the OCP R by interacting with the C-terminal domain of OCP 8. The biological functions of HCPs, which are grouped into nine distinct clades 7 , are largely unknow...

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Carotenoid profile of three microalgae/cyanobacteria species with peroxyl radical scavenger capacity

Cited by 83 publications

References 36 publications

Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Effects of Potassium Chloride‐Induced Stress on the Carotenoids Canthaxanthin, Astaxanthin, and Lipid Accumulations in the Green Chlorococcal Microalga Strain TISTR 9500

Carotenoids from Cyanobacteria: A Biotechnological Approach for the Topical Treatment of Psoriasis

Genomic Survey of Salt Acclimation-Related Genes in the Halophilic Cyanobacterium Euhalothece sp. Z-M001

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