Carotenoid Production by Corynebacterium: The Workhorse of Industrial Amino Acid Production as Host for Production of a Broad Spectrum of C40 and C50 Carotenoids

Henke, Nadja A.; Peters‐Wendisch, Petra; Wendisch, Volker F.

doi:10.5772/67631

Cited by 5 publications

(4 citation statements)

References 71 publications

(104 reference statements)

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“…3b). This might be due to accumulation of tricarboxylic acid cycle metabolites with subsequent conversion to other products such as carotenoids (Henke et al 2017; Tao et al 2011).…”

Section: Resultsmentioning

confidence: 99%

Cultivation technology development of Rhodothermus marinus DSM 16675

et al. 2019

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This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LBmalt) resulted in higher cell density (OD620 = 6.6) than use of MBmalt (OD620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD620 = 20) in LBmalt and eightfold (OD620 = 14) in MBmalt. In both single and sequential batches, the production of carotenoids and EPSs using LBmalt was detected in the exponential phase and stationary phase, respectively, while in MBmalt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity (QE) of 0.67 (mg/L h) in MBmalt and the polymer contained xylose. In LB, QE was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.Electronic supplementary materialThe online version of this article (10.1007/s00792-019-01129-0) contains supplementary material, which is available to authorized users.

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“…3b). This might be due to accumulation of tricarboxylic acid cycle metabolites with subsequent conversion to other products such as carotenoids (Henke et al 2017; Tao et al 2011).…”

Section: Resultsmentioning

confidence: 99%

Cultivation technology development of Rhodothermus marinus DSM 16675

et al. 2019

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“…S2 and S3), a siderophore belonging to hydroxamate group (Ronan et al 2018), and the rare C50 carotenoid decaprenoxanthin (Fig. S4 and S5), a pigment used for coloration of food, feed and beverages (Henke et al 2017).…”

Section: Genome and Secondary Metabolic Gene Clustersmentioning

confidence: 99%

“…The identi cation of these endophytic strains is currently based on 16S rRNA gene sequencing, even though this method is unable to characterize the different species in this genus. The whole genome of strain ER1/6 T was previously sequenced and annotation data was deposited at DDBJ/EMBL/GenBank under the accession number MJAK00000000 (17). This sequence was also used to annotate genes related to secondary metabolite biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Curtobacterium uspiensis sp. nov., an endophytic bacterium isolated from Citrus sinensis (sweet orange) in Brazil

Araújo

Belmonte

Dourado

et al. 2023

Preprint

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Endophytic bacteria were isolated from Citrus plants and based on the similarity of 16S rRNA gene sequence, 20 isolates were included in the genus Curtobacterium in the family Microbacteriaceae, class Actinobacteria. Amplified Fragment Length Polymorphism (AFLP) analysis indicated that these strains formed four clusters with low variability that were separated from Curtobacterium flaccumfaciens. The isolates were Gram-positive, non-motile, non-spore forming, pale-yellow to orange-pink colonies. Analysis of eleven isolates showed that the major fatty acids of these strains were anteiso-C15:0, anteiso-C17:0 and iso-C16:0 and MK-9 and MK-8 as the major isoprenoid quinone, supporting the affiliation into Curtobacterium genus. The similarity of 16S rRNA gene between these isolates ranged from 99.9 to 100%, suggesting that this endophytic population present a low variability and similarity to species with validity published names within this genus, forming a distinct group in the phylogenetic tree. The DNA–DNA relatedness values to closest species were less than 48% for ER1/6T, suggesting that this strain does not belong to already described species. Analysis of the ER1/6T genome detected genes predicted to be involved in secondary metabolites synthesis, such as siderophore Desferrioxamine-like, bacteriocin Lactococcin 972-like, and terpene C50 carotenoid-like. Based on genome sequencing, the G + C content of the strain ER1/6T was 72.2%. Therefore, the polyphasic taxonomic characterization demonstrated that the strains ER1/6T, ER1.4/2, SR4/1 and SR4/8 dominant in the citrus tissues, represent a new species of the genus Curtobacterium, for which the name Curtobacterium uspiensis sp. nov. is proposed, with strain ER1/6T = CBMAI 2131 as the type strain.

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“…Therefore, to overcome the challenges that hinder the large-scale preparations of avonoid glucosides, the engineering of microorganisms such as Escherichia coli, Saccharomyces cerevisiae, and C. glutamicum has been employed for the biosynthesis of these natural products [22,23]. C. glutamicum in particular, possesses a cell factory that is enhanced for the industrial-scale synthesis of products, hence it has been referred to as the workhorse of the biotechnology industry [24]. Thus making it a better platform for the cost-effective synthesis of avonoid glucosides on an industrial scale.…”

mentioning

confidence: 99%

Biosynthesis of Apigenin Glucosides in Engineered Corynebacterium glutamicum

Amoah,

Thapa,

et al. 2024

J. Microbiol. Biotechnol.

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Glucosylation is a well-known approach to improve the solubility, pharmacological, and biological properties of flavonoids, making flavonoid glucosides a target for large-scale biosynthesis. However, the low yield of products coupled with the requirement of expensive UDP-sugars limits the application of enzymatic systems for large-scale. C. glutamicum is a Gram-positive and generally regarded as safe (GRAS) bacteria frequently employed for the large-scale production of amino acids and bio-fuels. Due to the versatility of its cell factory system and its non-endotoxin producing properties, it has become an attractive system for the industrial-scale biosynthesis of alternate products. Here, we explored the cell factory of C. glutamicum for efficient glucosylation of flavonoids using apigenin as a model flavonoid, with the heterologous expression of a promiscuous glycosyltransferase, YdhE from Bacillus licheniformis and the endogenous overexpression of C. glutamicum genes galU1 encoding UDP-glucose pyrophosphorylase and pgm encoding phosphoglucomutase involved in the synthesis of UDP-glucose to create a C. glutamicum cell factory system capable of efficiently glucosylation apigenin with a high yield of glucosides production. Consequently, the production of various apigenin glucosides was controlled under different temperatures yielding almost 4.2 mM of APG1(apigenin-4'-O-β-glucoside) at 25°C, and 0.6 mM of APG2 (apigenin-7-O-β-glucoside), 1.7 mM of APG3 (apigenin-4',7-O-β-diglucoside) and 2.1 mM of APG4 (apigenin-4',5-O-β-diglucoside) after 40 h of incubation with the supplementation of 5 mM of apigenin and 37°C. The cost-effective developed system could be used to modify a wide range of plant secondary metabolites with increased pharmacokinetic activities on a large scale without the use of expensive UDP-sugars.

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Carotenoid Production by Corynebacterium: The Workhorse of Industrial Amino Acid Production as Host for Production of a Broad Spectrum of C40 and C50 Carotenoids

Cited by 5 publications

References 71 publications

Cultivation technology development of Rhodothermus marinus DSM 16675

Cultivation technology development of Rhodothermus marinus DSM 16675

Curtobacterium uspiensis sp. nov., an endophytic bacterium isolated from Citrus sinensis (sweet orange) in Brazil

Biosynthesis of Apigenin Glucosides in Engineered Corynebacterium glutamicum

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