Carotenoid biosynthesis in extremophilic Deinococcus–Thermus bacteria

Teng, Bing; Hua, Yuejin

doi:10.1016/j.tim.2010.07.007

Cited by 176 publications

(129 citation statements)

References 60 publications

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“…Bacteroidetes have been found in the intestinal microbiota of various organisms (Flint, Bayer, Rincon, Lamed & White 2008) and participate in fermentative metabolism and degradation of oligosaccharides derived from plant material (Van der Meulen, Makras, Verbrugghe, Adriany & De Vuyst 2006). DeinococcusThermus species are known for their resistance to extreme stresses, such as radiation, oxidation, desiccation and high temperature (Tian & Hua 2010), but their functional role in the gut of C. idellus deserves further research. Verrucomicrobia species are most commonly detected in aquatic environments, and these bacteria seem to be well adapted to live with eukaryotes, as the genome of some Verrucomicrobial species contains a protein secretion system, which mediates interactions between eukaryotic and bacterial cells (Wagner & Horn 2006).…”

Section: H64(kf003201)mentioning

confidence: 99%

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Zhong

Wirth

et al. 2014

Aquac Res

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Traditional culture-based technique and 16S rDNA sequencing method were used to investigate the mucosa-associated autochthonous microbiota of grass carp (Ctenopharyngodon idellus). Twenty-one phylotypes were detected from culturable microbiota, with Aeromonas, Shewanella, Lactococcus, Serratia, Brevibacillus, Delftia, Pseudomonas, Pantoea, Enterobacter, Buttiauxella and Yersinia as their closest relatives. Genomic DNA was directly extracted from the gut mucosa of C. idellus originating from six different geographical regions, and used to generate 609 random bacterial clones from six clone libraries and 99 archaeal clones from one library, which were grouped into 67 bacterial and four archaeal phylotypes. Sequence analysis revealed that the intestinal mucosa harboured a diversified bacterial microbiota, where Proteobacteria, Firmicutes and Bacteroidetes were dominant, followed by Actinobacteria, Verrucomicrobia and DeinococcusThermus. The autochthonous bacterial communities in the gut mucosa of fish from different aquatic environments were not similar (C s < 0.80), but c-Proteobacteria was a common bacterial class. In comparison to bacterial communities, the archaeal community obtained from one library consisted of Crenarchaeota and Euryarchaeota. These results demonstrate that molecular methods facilitate culture-independent studies, and that fish gut mucosa harbours a larger bacterial diversity than previously recognized. The grass carp intestinal habitat selects for specific bacterial taxa despite pronounced differences in host environments.

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Section: H64(kf003201)mentioning

confidence: 99%

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Zhong

Wirth

et al. 2014

Aquac Res

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“…strains RP and TP show most similarity to the carotenoids deoxyflexixanthin and 1′-β-glucopyranosyl-3,4,3′, 4′-tetradehydro-1′, 2′-dihydro-β, ψ-caroten-2-one, both of which are carotenoid glycoside esters, so tentatively, the carotenoid extracts of strains RP and TP may be assigned as belonging to the family of carotenoid glycosides related to those two carotenoids. Similarly, the absorption spectra and maxima of strain YY show most similarity to the group of zeaxanthin and zeaxanthin derivates, so most probably, it belongs to zeaxanthin family [22]. Though a little work on the antioxidant property of Thermus filiformis carotenoid extract has been done [21], studies on the antioxidant properties of carotenoids belonging to this phylum are limited and analysis of antioxidant properties of strains related to Meiothermus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Again, the zeaxanthin and thermobiszeaxanthin esters have carbon number ranges of 59-82, whereas deoxyflexixanthin has a carbon number of 40 and 1′-β-glucopyranosyl-3,4,3′, 4′-tetradehydro-1′, 2′-dihydro-β, ψ-caroten-2-one has a carbon number of 46. Though all of them are glycoside esters, the molecular mass are widely different and so are the differences in activity among the three strain extracts [18,22].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant properties of the carotenoid extracts of three Deinococcus–Thermus phylum bacteria, Meiothermus sp. strains RP and TP and Thermus sp. strain YY from Paniphala hot spring, India

Mukherjee

Bose

Mukhopadhyay

2017

Nutrire

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Background: The purpose of the study was to extract carotenoids from thermophilic bacteria which show efficient antioxidant and protein oxidation inhibition properties, characterize and identify those isolates, extract the carotenoids in different solvents, quantify the carotenoids and perform concentration-dependent and solventdependent quantitative assays validated and analysed by appropriate statistical tests.

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“…(30). Thermus-Deinococcus bacteria are usually pigmented yellow or red because of carotenoid production (31), and HB27 is no exception. The terminal steps of the synthesis of this yellow pigment are encoded on pTT27 (30).…”

mentioning

confidence: 99%

Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain

Ohtani

Tomita

Itaya

2016

Appl Environ Microbiol

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Stepwise deletions in the only plasmid in Thermus thermophilus HB27, megaplasmid pTT27, showed that two distantly located loci were important for maintenance of the plasmid. One is a minimum replicon including one gene, repT, coding a replication initiator, and the other encodes subunits of class I ribonucleotide reductase (RNR) for deoxynucleoside triphosphate (dNTP) synthesis. Since the initiator protein, RepT, bound to direct repeats downstream from its own gene, it was speculated that a more-downstream A؉T-rich region, which was critical for replication ability, could be unwound for replication initiation. On the other hand, the class I RNR is not necessarily essential for cell growth, as evidenced by the generation of the plasmid-free strain by the loss of pTT27. However, the plasmid-free strain culture has fewer viable cells than the wild-type culture, probably due to a dNTP pool imbalance in the cell. This is because of the introduction of the class I RNR genes or the supplementation of 5=-deoxyadenosylcobalamin, which stimulated class II RNR encoded in the chromosome, resolved the decrease in the number of viable cells in the plasmid-free strain. Likewise, these treatments dramatically enhanced the efficiency of transformation by exogenous plasmids and the stability of the plasmids in the strain. Therefore, the class I RNR would enable the stable maintenance of plasmids, including pTT27, as a result of genome replication normalized by reversing the dNTP pool imbalance. The generation of this plasmid-free strain with great natural competence and its analysis in regard to exogenous plasmid maintenance will expand the availability of HB27 for thermophilic cell factories.T hermus spp. are extremely thermophilic bacteria that can grow at high temperatures from 50 to 82°C. Their thermophilic enzymes, e.g., DNA polymerase for PCR, have been used in industrial applications, and their potential as cell factories to produce enzymes from other thermophiles has been demonstrated (1). The bacteria themselves have also been extensively investigated as model organisms for systems biology including structural genomics (1, 2), and their genomic DNAs, with GϩC contents as high as about 70%, are an interesting target for synthetic biology (3). Thermus-related studies have been encouraged by the existence of an established genetic engineering system based on natural competence (4-6), with thermostabilized drug resistance markers (7-10) and other thermostable genetic tools (1,(11)(12)(13)(14)(15)(16).Complete genomic sequences of T. scotoductus SA-01, T. oshimai JL-2, Thermus sp. strain CCB_US3_UF1, T. aquaticus Y51MC23, T. thermophilus strains HB8, HB27, SG0.5JP17-16, and JL-18, and others are available online. Thermus spp. often harbor a megaplasmid larger than 100 kbp, whereas in some strains, the megaplasmid genes seem to be transferred to the chromosome (17, 18). Similar megaplasmids have been observed in other bacteria of the Thermus-Deinococcus group showing resistance to extreme stresses such as high temperature, rad...

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Carotenoid biosynthesis in extremophilic Deinococcus–Thermus bacteria

Cited by 176 publications

References 60 publications

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Antioxidant properties of the carotenoid extracts of three Deinococcus–Thermus phylum bacteria, Meiothermus sp. strains RP and TP and Thermus sp. strain YY from Paniphala hot spring, India

Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain

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