Carob extract induces spermatogenesis in an infertile mouse model via upregulation of Prm1, Plzf, Bcl-6b, Dazl, Ngn3, Stra8, and Smc1b

Ghorbaninejad, Zeynab; Eghbali, Atiyeh; Ghorbaninejad, Mahsa; Ayyari, Mahdi; Żuchowski, Jerzy; Kowalczyk, Mariusz; Baharvand, Hossein; Shahverdi, Abdolhossein; Eftekhari‐Yazdi, Poopak; Esfandiari, Fereshteh

doi:10.1016/j.jep.2022.115760

Cited by 6 publications

(12 citation statements)

References 48 publications

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“…Fourteen peaks were tentatively identified as sugars derivatives, namely, acylated disaccharide [ 5 , 29 ]. Peaks 10 , 16 , 17 , and 18 , with [M-H] − at m / z 411.1518, corresponding to a molecular formula of C 16 H 28 O 12 , were attributed to (iso)butyryl-dihexose isomers [ 29 ]. Other isomers of the same compound were also detected as formic acid adduct (peaks 3 and 15 ).…”

Section: Resultsmentioning

confidence: 99%

“…Other isomers of the same compound were also detected as formic acid adduct (peaks 3 and 15 ). Peaks 11 , 13 , and 14 , with [M-H] − at m / z 427.1466, were attributed to the formic acid adduct of (iso)butyryl-hexose-pentose corresponding to a molecular formula of C 15 H 26 O 11 [ 29 ]. Peaks 22 and 23 , with [M-H] − at m / z 441.1615, were attributed to the formic acid adduct of acilated-hexose-pentose, corresponding to a molecular formula of C 16 H 28 O 11 [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Peaks 11 , 13 , and 14 , with [M-H] − at m / z 427.1466, were attributed to the formic acid adduct of (iso)butyryl-hexose-pentose corresponding to a molecular formula of C 15 H 26 O 11 [ 29 ]. Peaks 22 and 23 , with [M-H] − at m / z 441.1615, were attributed to the formic acid adduct of acilated-hexose-pentose, corresponding to a molecular formula of C 16 H 28 O 11 [ 29 ]. Peaks 27 and 28 , with [M-H] − at m / z 461.1296, were attributed to the formic acid adduct of acilated-hexose-pentose corresponding to a molecular formula of C 18 H 24 O 11 [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Nineteen compounds were identified as hydroxybenzoic acid derivatives, mainly galloyl glucose derivatives (gallotannins), due to the typical fragment ion at m/z 169, which was associated with the gallic acid unit [ 5 , 29 ]. Peak 2 , with [M−H] − at m/z 169.01476, was absolutely identified as gallic acid by comparing with its commercial standard.…”

Section: Resultsmentioning

confidence: 99%

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Ceratonia siliqua L. Pod Extract: From Phytochemical Characterization to Liposomal Formulation and Evaluation of Behaviour in Cells

et al. 2023

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The formulation of plant extracts in phospholipid vesicles is a promising strategy to exploit their biological properties while solving problems related to poor solubility in water, high instability, and low skin permeation and retention time. In this study, Ceratonia siliqua ripe pods were used for the preparation of a hydro-ethanolic extract, which showed antioxidant properties owing to the presence of biologically active compounds identified by liquid chromatography–mass spectrometry (e.g., hydroxybenzoic acid and flavonoid derivatives). To improve the applicability of the extract in therapy, a topical formulation based on liposomes was explored. The vesicles were characterized by small size (around 100 nm), negative charge (−13 mV), and high entrapment efficiency (>90%). Furthermore, they displayed both spherical and elongated shapes, with oligolamellar structure. Their biocompatibility was demonstrated in cells, including erythrocytes and representative skin cell lines. The antioxidant activity of the extract was proved by the scavenging of free radicals, the reduction of ferric ions, and the protection of skin cells from oxidative damage.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Ceratonia siliqua L. Pod Extract: From Phytochemical Characterization to Liposomal Formulation and Evaluation of Behaviour in Cells

et al. 2023

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“…This suggests, according to the authors, that the plant has an anti-apoptotic and inductive role in the gene expression regulating the cell cycle of spermatogenesis involving Sertoli and Leydig cells (p<0.05). Because of this, this plant holds great promise for treating azoospermia, especially those brought on by cancer and the side effects of chemotherapy (Ghorbaninejad et al 2023).…”

Section: Resultsmentioning

confidence: 99%

Modern possibilities of phytotherapy for male infertility from the standpoint of evidence-based medicine

Bratchikov,

Tyuzikov,

Grekov

2023

RRP

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Introduction: Male infertility is an topical medical and social problem of modern reproductive medicine. Its pharmacotherapy is often empirical in nature, and the most popular method remains the use of various herbal substances (phytotherapy), the effectiveness of which remains still understudied in the framework of evidence-based medicine. Materials and Methods: The results of research, thematic, systematic and Cochrane reviews and meta-analyses were searched in Medline/PubMed medical databases over the past 5 years using the search queries "plants male infertility", "plants sperm", "phytotherapy male infertility", "phytomedicinal therapeutics male infertility", "systematic review", "meta-analysis", and "review". Results: The vast majority of herbal substances offered for the treatment of male infertility demonstrate insufficient or contradictory evidence base for their clinical effectiveness, although some of them can be very useful pharmacotherapeutic options in the combined therapy of male infertility. Conclusion: Not all plant substances with a "reproductive effect" positioned in them actually have proven reproductive effects in studies in humans, therefore, the choice of phytotherapeutic agents in the treatment of idiopathic male infertility should be currently approached extremely carefully, especially in cases when we choose phytotherapy as an option for empirical monotherapy of male reproductive disorders.

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The Effect of Carob (Ceratonia Siliqua) on Sperm Parameters of Infertile Men: Systematic Review and Meta-Analysis of Randomized Controlled Trials

Karaahmet,

Gençtürk,

Kınık

2024

Reprod. Sci.

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Carob (Ceratonia siliqua) supplements can increase sperm quality. This study aimed to summarize the available evidence about the effects of carob (Ceratonia siliqua) supplements on sperm quality and reproductive hormones in infertile men. Systematic searches of five databases were conducted from inception to October 20, with the last update on November 20, 2023. Randomized clinical trials (RCTs) that compared carob (Ceratonia siliqua) supplements with nonintervention control groups on infertile man. Risk of bias and certainty of evidence were assessed by the Cochrane risk of bias tool 2. Summary effect size measures were calculated using a random-effects model estimation and are reported as standardized mean differences and 95% confidence intervals. Reporting followed the PRISMA guidelines. The analysis included four studies involving 236 infertile men. It was found that sperm motility of infertile men improved after carob intervention (MD:11.30, 95% CI:5.97 to 16.64, Z = 4.15, p < 0.00001), and there was a significant difference compared to control groups. The effect size of carob on semen quantity in infertile men was positive, and the relationship was statistically significant (MD:5.42, 95% CI:1.58 to 9.42, Z = 2.77, p = 0.006). When hormone parameters of infertile men were analyzed, the MDA (malondialdehyde) value decreased compared to the control group (MD = -4.81, 95% CI: -10.18 to 0.56, Z = 1.76, p = 0.08), and there was a significant difference between them. Carob (Ceratonia siliqua) supplements was associated with improvement in sperm quality compared with nonintervention control groups in infertile man. However, high-quality, larger RCTs are required to draw more definitive conclusions.

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Carob extract induces spermatogenesis in an infertile mouse model via upregulation of Prm1, Plzf, Bcl-6b, Dazl, Ngn3, Stra8, and Smc1b

Cited by 6 publications

References 48 publications

Ceratonia siliqua L. Pod Extract: From Phytochemical Characterization to Liposomal Formulation and Evaluation of Behaviour in Cells

Ceratonia siliqua L. Pod Extract: From Phytochemical Characterization to Liposomal Formulation and Evaluation of Behaviour in Cells

Modern possibilities of phytotherapy for male infertility from the standpoint of evidence-based medicine

The Effect of Carob (Ceratonia Siliqua) on Sperm Parameters of Infertile Men: Systematic Review and Meta-Analysis of Randomized Controlled Trials

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