Carnosine improves diabetic retinopathy via the MAPK/ERK pathway

Abstract

Diabetic retinopathy (DR) is one of the most common causes of blindness in developed countries. Due to its asymptomatic onset and progressive disease course, DR is typically diagnosed at a late stage when treatment options are limited and therefore often results in irreversible blindness. Studies have demonstrated that carnosine may prevent and treat DR. In a previous study, the positive effect of carnosine on DR was determined and it was revealed that there may be an association between carnosine and the mitogen-activated protein kinase (MAPK)/extracellular signal related kinase (ERK) signaling pathway. To assess the interaction between carnosine and the MAPK/ERK signaling pathway, changes in PKC, ERK and p-ERK expression was assessed in diabetic rats following treatment with carnosine, PD98059 or U46619 via reverse transcription-quantitative polymerase chain reaction and western blotting. The results demonstrated that the expression of ERK and p-ERK were significantly suppressed following treatment with carnosine, but no significant effect on the expression of PKC was identified, which indicates that suppressing the activation of the MAPK/ERK signaling pathway may serve an important role in carnosine-induced DR prevention and treatment.

Study on the transcriptome for breast muscle of chickens and the function of key gene RAC2 on fibroblasts proliferation

“…Members of the Mitogen-activated Protein Kinases ( MAPKs ) superfamily are important downstream signaling molecules of PAKs [ 50 , 51 ]. MAPKs are abundant in eukaryotic cells, which can transmit extracellular signals to the nucleus and regulate various physiological and biochemical processes in cells [ 52 ]. As a member of MAPK signaling pathway, MAPK8 , also known as JNK, JNK1 and SAPK1 , participates in many biological and molecular processes, such as cell proliferation, differentiation and apoptosis [ 53 – 55 ].…”

Natural and Synthetic Agents Targeting Reactive Carbonyl Species against Metabolic Syndrome

“…An example of this is the study by Y Guo et al that demonstrated how diabetic retinopathy was reversed in rats after administration of carnosine. Moreover, glucose uptake in skeletal muscle cells was enhanced, which suggested reversal of glucolipotoxicity [ 64 ]. They also highlighted that this reversal was facilitated by the MAPK/ERK pathway, which is a pathway implicated in various pathogenetic mechanisms.…”

Karnozi̇ni̇n Sağlik Üzeri̇ne Etki̇leri̇

“…Bu çalışma ile diyabetik komplikasyonları o nleme ve tedavi etmede potansiyel çoklu koruyucu olduğ u ifade edilmiştir (44). Karnozinin diyabetik retinopatiyi (DR) o nleyebileceğ ini ve tedavi edebileceğ i du şu nu lerek diyabetik sıçanlar u zerinde yapılan çalışmada mu dahale ğrubuna 25 mcmol/L karnozin verilerek mitojen -aktive olmuş protein kinaz (MAPK)/hu cre dışı sinyale bağ lı kinaz (ERK) sinyal yolunun aktivasyonunun baskılandığ ı ğo ru lmu ştu r. Bu nedenle karnozinin DR'yi o nleme ve tedavi etmede o nemli bir rol oynayabileceğ i ifade edilmiştir (45). Diyabetik nefropati, kronik bo brek yetmezliğ ine yol açabilecek diyabetin o nemli bir komplikasyonudur.…”